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Stem cell conditioned culture media attenuated albumin-induced epithelial-mesenchymal transition in renal tubular cells.

Hu J, Zhu Q, Li PL, Wang W, Yi F, Li N - Cell. Physiol. Biochem. (2015)

Bottom Line: Albumin induced EMT as shown by significant decreases in levels of epithelial marker E-cadherin, increases in mesenchymal markers fibroblast-specific protein 1 and α-smooth muscle actin, and elevations in collagen I.Albumin also increased the levels of pro-inflammatory factor monocyte chemoattractant protein-1 (MCP)-1 by nearly 30 fold compared with control.SCM almost abolished albumin-induced increase of MCP-1.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology & Toxicology, Virginia Commonwealth University School of Medicine, Richmond, VA, USA.

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Effect of SCM on albumin-induced increase in mesenchymal marker α-SMA by Western blot analysis. Upper panel: Representative gel documents; Lower panel: summarized data. Ctrl = control cells treated with CCM, Alb = cells treated with albumin + CCM, Alb+SCM = cells treated with albumin + SCM. n=6 batches of cells, *P<0.05 vs. other groups.
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Figure 3: Effect of SCM on albumin-induced increase in mesenchymal marker α-SMA by Western blot analysis. Upper panel: Representative gel documents; Lower panel: summarized data. Ctrl = control cells treated with CCM, Alb = cells treated with albumin + CCM, Alb+SCM = cells treated with albumin + SCM. n=6 batches of cells, *P<0.05 vs. other groups.

Mentions: Cells were treated with 1) control culture media (CCM), 2) rat albumin + CCM, and 3) rat albumin + SCM. Western blot analysis showed that protein expression of epithelial cell marker E-Cadherin was much lower in albumin-treated cells than that in control cells, whereas its expression in SCM-treated cells were recovered to levels similar to that in control cells (Fig. 1). In contrast, the protein levels of mesenchymal cell markers FSP-1 (Fig. 2) and α-SMA (Fig. 3) were both much higher in albumin-treated cells than that in control cells, whereas the levels of FSP-1 and α-SMA in SCM-treated cells were similar to that in control cells. These results indicated that albumin reduced epithelial marker and stimulated the mesenchymal markers and that SCM blocked the albumin-induced changes in EMT markers.


Stem cell conditioned culture media attenuated albumin-induced epithelial-mesenchymal transition in renal tubular cells.

Hu J, Zhu Q, Li PL, Wang W, Yi F, Li N - Cell. Physiol. Biochem. (2015)

Effect of SCM on albumin-induced increase in mesenchymal marker α-SMA by Western blot analysis. Upper panel: Representative gel documents; Lower panel: summarized data. Ctrl = control cells treated with CCM, Alb = cells treated with albumin + CCM, Alb+SCM = cells treated with albumin + SCM. n=6 batches of cells, *P<0.05 vs. other groups.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401473&req=5

Figure 3: Effect of SCM on albumin-induced increase in mesenchymal marker α-SMA by Western blot analysis. Upper panel: Representative gel documents; Lower panel: summarized data. Ctrl = control cells treated with CCM, Alb = cells treated with albumin + CCM, Alb+SCM = cells treated with albumin + SCM. n=6 batches of cells, *P<0.05 vs. other groups.
Mentions: Cells were treated with 1) control culture media (CCM), 2) rat albumin + CCM, and 3) rat albumin + SCM. Western blot analysis showed that protein expression of epithelial cell marker E-Cadherin was much lower in albumin-treated cells than that in control cells, whereas its expression in SCM-treated cells were recovered to levels similar to that in control cells (Fig. 1). In contrast, the protein levels of mesenchymal cell markers FSP-1 (Fig. 2) and α-SMA (Fig. 3) were both much higher in albumin-treated cells than that in control cells, whereas the levels of FSP-1 and α-SMA in SCM-treated cells were similar to that in control cells. These results indicated that albumin reduced epithelial marker and stimulated the mesenchymal markers and that SCM blocked the albumin-induced changes in EMT markers.

Bottom Line: Albumin induced EMT as shown by significant decreases in levels of epithelial marker E-cadherin, increases in mesenchymal markers fibroblast-specific protein 1 and α-smooth muscle actin, and elevations in collagen I.Albumin also increased the levels of pro-inflammatory factor monocyte chemoattractant protein-1 (MCP)-1 by nearly 30 fold compared with control.SCM almost abolished albumin-induced increase of MCP-1.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology & Toxicology, Virginia Commonwealth University School of Medicine, Richmond, VA, USA.

Show MeSH
Related in: MedlinePlus