Stem cell conditioned culture media attenuated albumin-induced epithelial-mesenchymal transition in renal tubular cells.
Bottom Line: Albumin induced EMT as shown by significant decreases in levels of epithelial marker E-cadherin, increases in mesenchymal markers fibroblast-specific protein 1 and α-smooth muscle actin, and elevations in collagen I.Albumin also increased the levels of pro-inflammatory factor monocyte chemoattractant protein-1 (MCP)-1 by nearly 30 fold compared with control.SCM almost abolished albumin-induced increase of MCP-1.
Affiliation: Department of Pharmacology & Toxicology, Virginia Commonwealth University School of Medicine, Richmond, VA, USA.Show MeSH
Related in: MedlinePlus
Mentions: Cells were treated with 1) control culture media (CCM), 2) rat albumin + CCM, and 3) rat albumin + SCM. Western blot analysis showed that protein expression of epithelial cell marker E-Cadherin was much lower in albumin-treated cells than that in control cells, whereas its expression in SCM-treated cells were recovered to levels similar to that in control cells (Fig. 1). In contrast, the protein levels of mesenchymal cell markers FSP-1 (Fig. 2) and α-SMA (Fig. 3) were both much higher in albumin-treated cells than that in control cells, whereas the levels of FSP-1 and α-SMA in SCM-treated cells were similar to that in control cells. These results indicated that albumin reduced epithelial marker and stimulated the mesenchymal markers and that SCM blocked the albumin-induced changes in EMT markers.
Affiliation: Department of Pharmacology & Toxicology, Virginia Commonwealth University School of Medicine, Richmond, VA, USA.