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Characterisation and comparison of temporal release profiles of nitric oxide generating donors.

Bradley SA, Steinert JR - J. Neurosci. Methods (2015)

Bottom Line: We found that donors such as NOC-5 and PAPA-NONOate decayed substantially within days, whereas SNP and GSNO showed greater stability releasing consistent levels of NO over days.In all donors tested, the amount of released NO differs between frozen and unfrozen stocks.Fluorescent and amperometric approaches to measure NO concentrations yield a wide range of levels.

View Article: PubMed Central - PubMed

Affiliation: MRC Toxicology Unit, Hodgkin Building, University of Leicester, Leicester LE1 9HN, UK.

No MeSH data available.


Related in: MedlinePlus

SNAP calibration of NO microsensors. (A) Graph depicting sequential additions of increasing concentrations of SNAP in 0.1 M copper sulphate and the equivalent concentration of NO produced in nM. Arrows indicate time points of SNAP addition. (B) Graph showing the mean calibration curves constructed from twice daily SNAP calibrations over three different weeks.
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fig0005: SNAP calibration of NO microsensors. (A) Graph depicting sequential additions of increasing concentrations of SNAP in 0.1 M copper sulphate and the equivalent concentration of NO produced in nM. Arrows indicate time points of SNAP addition. (B) Graph showing the mean calibration curves constructed from twice daily SNAP calibrations over three different weeks.

Mentions: The NOPF100 microsensor was calibrated using increasing concentrations of SNAP diluted in CuSO4, as recommended in the user manual provided by WPI. The output of the microsensor correlated linearly with the concentration of NO liberated from each SNAP application and a calibration curve was constructed (Fig. 1A and B). However, as stated previously, the probes are extremely sensitive to external factors, which is also demonstrated in this study. Fig. 1B shows the average calibration curve taken twice daily for a week on three different weeks showing a significant variation over that time. Therefore we would advise calibration of NO probes daily before use and after experimentation to avoid variation and to provide accurate determination of NO release during experimentation.


Characterisation and comparison of temporal release profiles of nitric oxide generating donors.

Bradley SA, Steinert JR - J. Neurosci. Methods (2015)

SNAP calibration of NO microsensors. (A) Graph depicting sequential additions of increasing concentrations of SNAP in 0.1 M copper sulphate and the equivalent concentration of NO produced in nM. Arrows indicate time points of SNAP addition. (B) Graph showing the mean calibration curves constructed from twice daily SNAP calibrations over three different weeks.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401449&req=5

fig0005: SNAP calibration of NO microsensors. (A) Graph depicting sequential additions of increasing concentrations of SNAP in 0.1 M copper sulphate and the equivalent concentration of NO produced in nM. Arrows indicate time points of SNAP addition. (B) Graph showing the mean calibration curves constructed from twice daily SNAP calibrations over three different weeks.
Mentions: The NOPF100 microsensor was calibrated using increasing concentrations of SNAP diluted in CuSO4, as recommended in the user manual provided by WPI. The output of the microsensor correlated linearly with the concentration of NO liberated from each SNAP application and a calibration curve was constructed (Fig. 1A and B). However, as stated previously, the probes are extremely sensitive to external factors, which is also demonstrated in this study. Fig. 1B shows the average calibration curve taken twice daily for a week on three different weeks showing a significant variation over that time. Therefore we would advise calibration of NO probes daily before use and after experimentation to avoid variation and to provide accurate determination of NO release during experimentation.

Bottom Line: We found that donors such as NOC-5 and PAPA-NONOate decayed substantially within days, whereas SNP and GSNO showed greater stability releasing consistent levels of NO over days.In all donors tested, the amount of released NO differs between frozen and unfrozen stocks.Fluorescent and amperometric approaches to measure NO concentrations yield a wide range of levels.

View Article: PubMed Central - PubMed

Affiliation: MRC Toxicology Unit, Hodgkin Building, University of Leicester, Leicester LE1 9HN, UK.

No MeSH data available.


Related in: MedlinePlus