Limits...
Complete genome sequence of canine astrovirus with molecular and epidemiological characterisation of UK strains.

Caddy SL, Goodfellow I - Vet. Microbiol. (2015)

Bottom Line: These viruses can also cause infection in a range of domestic and wild animal species.Sequencing of the capsid sequences from the four CaAstV strains found significant genetic heterogeneity, with only 80% amino acid identity between strains.The full genome sequence of two UK CaAstV strains was then determined, confirming that CaAstV conforms to the classic genome organisation of other astroviruses with ORF1a and ORF1b separated by a frameshift and ORF2 encoding the capsid protein.

View Article: PubMed Central - PubMed

Affiliation: Division of Virology, Department of Pathology, University of Cambridge, Addenbrookes Hospital, Cambridge CB2 2QQ, UK; Section of Virology, Faculty of Medicine, Imperial College London, St. Mary's Campus, London W2 1PG, UK. Electronic address: slc50@cam.ac.uk.

Show MeSH

Related in: MedlinePlus

Phylogenetic tree based complete genome nucleotide sequences of astroviruses from a range of mammalian species. GenBank accession numbers are listed for all sequences analysed, with the CaAstV sequences underlined. The tree was determined using the neighbour-joining method. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (100 replicates) is shown next to the branches. Evolutionary analyses were conducted in MEGA6.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4401448&req=5

fig0025: Phylogenetic tree based complete genome nucleotide sequences of astroviruses from a range of mammalian species. GenBank accession numbers are listed for all sequences analysed, with the CaAstV sequences underlined. The tree was determined using the neighbour-joining method. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (100 replicates) is shown next to the branches. Evolutionary analyses were conducted in MEGA6.

Mentions: A phylogenetic tree was constructed by multiple alignment of the full-length genome of the two CaAstV strains isolated in this study, and a number of astrovirus reference strains isolated from different mammalian species. This was achieved using MEGA6 software (Fig. 5).


Complete genome sequence of canine astrovirus with molecular and epidemiological characterisation of UK strains.

Caddy SL, Goodfellow I - Vet. Microbiol. (2015)

Phylogenetic tree based complete genome nucleotide sequences of astroviruses from a range of mammalian species. GenBank accession numbers are listed for all sequences analysed, with the CaAstV sequences underlined. The tree was determined using the neighbour-joining method. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (100 replicates) is shown next to the branches. Evolutionary analyses were conducted in MEGA6.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401448&req=5

fig0025: Phylogenetic tree based complete genome nucleotide sequences of astroviruses from a range of mammalian species. GenBank accession numbers are listed for all sequences analysed, with the CaAstV sequences underlined. The tree was determined using the neighbour-joining method. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (100 replicates) is shown next to the branches. Evolutionary analyses were conducted in MEGA6.
Mentions: A phylogenetic tree was constructed by multiple alignment of the full-length genome of the two CaAstV strains isolated in this study, and a number of astrovirus reference strains isolated from different mammalian species. This was achieved using MEGA6 software (Fig. 5).

Bottom Line: These viruses can also cause infection in a range of domestic and wild animal species.Sequencing of the capsid sequences from the four CaAstV strains found significant genetic heterogeneity, with only 80% amino acid identity between strains.The full genome sequence of two UK CaAstV strains was then determined, confirming that CaAstV conforms to the classic genome organisation of other astroviruses with ORF1a and ORF1b separated by a frameshift and ORF2 encoding the capsid protein.

View Article: PubMed Central - PubMed

Affiliation: Division of Virology, Department of Pathology, University of Cambridge, Addenbrookes Hospital, Cambridge CB2 2QQ, UK; Section of Virology, Faculty of Medicine, Imperial College London, St. Mary's Campus, London W2 1PG, UK. Electronic address: slc50@cam.ac.uk.

Show MeSH
Related in: MedlinePlus