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Complete genome sequence of canine astrovirus with molecular and epidemiological characterisation of UK strains.

Caddy SL, Goodfellow I - Vet. Microbiol. (2015)

Bottom Line: These viruses can also cause infection in a range of domestic and wild animal species.Sequencing of the capsid sequences from the four CaAstV strains found significant genetic heterogeneity, with only 80% amino acid identity between strains.The full genome sequence of two UK CaAstV strains was then determined, confirming that CaAstV conforms to the classic genome organisation of other astroviruses with ORF1a and ORF1b separated by a frameshift and ORF2 encoding the capsid protein.

View Article: PubMed Central - PubMed

Affiliation: Division of Virology, Department of Pathology, University of Cambridge, Addenbrookes Hospital, Cambridge CB2 2QQ, UK; Section of Virology, Faculty of Medicine, Imperial College London, St. Mary's Campus, London W2 1PG, UK. Electronic address: slc50@cam.ac.uk.

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Phylogenetic tree based on the full-length amino acid sequence of the capsid protein of CaAstVs. This includes the four UK strains identified in this study (underlined) and the seven strains previously sequenced and listed in GenBank. The tree was determined using the neighbour-joining method. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (100 replicates) is shown next to the branches. Evolutionary analyses were conducted in MEGA6.
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fig0010: Phylogenetic tree based on the full-length amino acid sequence of the capsid protein of CaAstVs. This includes the four UK strains identified in this study (underlined) and the seven strains previously sequenced and listed in GenBank. The tree was determined using the neighbour-joining method. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (100 replicates) is shown next to the branches. Evolutionary analyses were conducted in MEGA6.

Mentions: Evolutionary analysis of the four CaAstV sequences from the study, alongside the seven previously reported full-length CaAstV capsid sequences are presented in Fig. 2. This analysis indicated that the UK strains do not cluster, contrary to a previous study, which analysed CaAstV strains from a single city (Zhu et al., 2011). Each UK strain is distinct from each other, and whereas one strain clusters most closely with the Chinese strains, the remainder group with strains identified in Italy over a number of years.


Complete genome sequence of canine astrovirus with molecular and epidemiological characterisation of UK strains.

Caddy SL, Goodfellow I - Vet. Microbiol. (2015)

Phylogenetic tree based on the full-length amino acid sequence of the capsid protein of CaAstVs. This includes the four UK strains identified in this study (underlined) and the seven strains previously sequenced and listed in GenBank. The tree was determined using the neighbour-joining method. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (100 replicates) is shown next to the branches. Evolutionary analyses were conducted in MEGA6.
© Copyright Policy - CC BY
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401448&req=5

fig0010: Phylogenetic tree based on the full-length amino acid sequence of the capsid protein of CaAstVs. This includes the four UK strains identified in this study (underlined) and the seven strains previously sequenced and listed in GenBank. The tree was determined using the neighbour-joining method. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (100 replicates) is shown next to the branches. Evolutionary analyses were conducted in MEGA6.
Mentions: Evolutionary analysis of the four CaAstV sequences from the study, alongside the seven previously reported full-length CaAstV capsid sequences are presented in Fig. 2. This analysis indicated that the UK strains do not cluster, contrary to a previous study, which analysed CaAstV strains from a single city (Zhu et al., 2011). Each UK strain is distinct from each other, and whereas one strain clusters most closely with the Chinese strains, the remainder group with strains identified in Italy over a number of years.

Bottom Line: These viruses can also cause infection in a range of domestic and wild animal species.Sequencing of the capsid sequences from the four CaAstV strains found significant genetic heterogeneity, with only 80% amino acid identity between strains.The full genome sequence of two UK CaAstV strains was then determined, confirming that CaAstV conforms to the classic genome organisation of other astroviruses with ORF1a and ORF1b separated by a frameshift and ORF2 encoding the capsid protein.

View Article: PubMed Central - PubMed

Affiliation: Division of Virology, Department of Pathology, University of Cambridge, Addenbrookes Hospital, Cambridge CB2 2QQ, UK; Section of Virology, Faculty of Medicine, Imperial College London, St. Mary's Campus, London W2 1PG, UK. Electronic address: slc50@cam.ac.uk.

Show MeSH
Related in: MedlinePlus