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Trps1 regulates biliary epithelial-mesenchymal transition and has roles during biliary fibrosis in liver grafts: a preliminary study.

Zhe C, Yu F, Tian J, Zheng S - PLoS ONE (2015)

Bottom Line: Mesenchymal markers were seen in biliary epithelial cells (BECs), with collagen deposited around the bile duct.Expression of epithelial marker mRNAs and proteins in HIBECs decreased with prolonged cold preservation (CP), while mesenchymal marker expression increased.Expression of E-cadherin was increased in HIBECs following Trps1 adenovirus infection and CP/reperfusion injury (CPRI), with vimentin expression levels reduced and CPRI-mediated epithelial-mesenchymal transition (EMT) inhibited.

View Article: PubMed Central - PubMed

Affiliation: Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, No. 29 Gaotanyan Road, Shapingba District, Chongqing, 400038, China.

ABSTRACT

Objective: To investigate the role(s) of Trps1 in non-anastomotic biliary stricture (NABS) following liver transplantation.

Methods: Immunohistochemical and histological techniques were used to detect Trps1, E-cadherin, CK19, vimentin, α-SMA, and collagen deposition. Human intrahepatic biliary epithelial cells (HIBECs) were infected with a Trps1 adenovirus, or transfected with Trps1 short-interfering RNAs (siRNAs). Reverse transcription polymerase chain reaction (RT-PCR) assays and western blotting were used to determine expression levels of epithelial and mesenchymal markers, and Trps1 in HIBECs.

Results: Expression of Trps1 and epithelial markers was down-regulated or absent in NABS liver samples. Mesenchymal markers were seen in biliary epithelial cells (BECs), with collagen deposited around the bile duct. Trps1 expression positively correlated with epithelial markers. Expression of epithelial marker mRNAs and proteins in HIBECs decreased with prolonged cold preservation (CP), while mesenchymal marker expression increased. A 12-h CP period led to increased Trps1 mRNA and protein levels. Expression of E-cadherin was increased in HIBECs following Trps1 adenovirus infection and CP/reperfusion injury (CPRI), with vimentin expression levels reduced and CPRI-mediated epithelial-mesenchymal transition (EMT) inhibited. Transfection of HIBECs with Trps1 siRNAs in conjunction with CPRI revealed that E-cadherin expression was decreased, vimentin expression was increased, and CPRI-mediated EMT was promoted.

Conclusion: Trps1 is involved in NABS pathogenesis following liver transplantation and negatively correlates with BEC EMT and biliary fibrosis in liver grafts. Trps1 demonstrates antagonistic effects that could reverse EMT.

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Related in: MedlinePlus

Scratch assays examining the effects of CPRI on the migratory capacity of HIBECs.A: normal HIBECs (×200 magnification); B: HIBECs subjected 24 h of CP and RI for 1 h (×200 magnification); C: 0 h after scratching normal HIBECs (×100 magnification); D: 0 h after scratching HIBECs subjected to CPRI (×100 magnification); D: 12 h after scratching normal HIBECs (×100 magnification); E: 12 h after scratching HIBECs subjected to CPRI (×100 magnification).
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pone.0123233.g001: Scratch assays examining the effects of CPRI on the migratory capacity of HIBECs.A: normal HIBECs (×200 magnification); B: HIBECs subjected 24 h of CP and RI for 1 h (×200 magnification); C: 0 h after scratching normal HIBECs (×100 magnification); D: 0 h after scratching HIBECs subjected to CPRI (×100 magnification); D: 12 h after scratching normal HIBECs (×100 magnification); E: 12 h after scratching HIBECs subjected to CPRI (×100 magnification).

Mentions: Following a 12-h CP period and 1 h of RI, normally ovoid HIBECs were transformed into fibroblast-like cells with a spindle morphology. Additionally, their cell migratory speed was significantly increased from 8.3 ± 2.2 μm/h to 32.5 ± 4.7 μm/h (p = 0.02; Fig 1).


Trps1 regulates biliary epithelial-mesenchymal transition and has roles during biliary fibrosis in liver grafts: a preliminary study.

Zhe C, Yu F, Tian J, Zheng S - PLoS ONE (2015)

Scratch assays examining the effects of CPRI on the migratory capacity of HIBECs.A: normal HIBECs (×200 magnification); B: HIBECs subjected 24 h of CP and RI for 1 h (×200 magnification); C: 0 h after scratching normal HIBECs (×100 magnification); D: 0 h after scratching HIBECs subjected to CPRI (×100 magnification); D: 12 h after scratching normal HIBECs (×100 magnification); E: 12 h after scratching HIBECs subjected to CPRI (×100 magnification).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401436&req=5

pone.0123233.g001: Scratch assays examining the effects of CPRI on the migratory capacity of HIBECs.A: normal HIBECs (×200 magnification); B: HIBECs subjected 24 h of CP and RI for 1 h (×200 magnification); C: 0 h after scratching normal HIBECs (×100 magnification); D: 0 h after scratching HIBECs subjected to CPRI (×100 magnification); D: 12 h after scratching normal HIBECs (×100 magnification); E: 12 h after scratching HIBECs subjected to CPRI (×100 magnification).
Mentions: Following a 12-h CP period and 1 h of RI, normally ovoid HIBECs were transformed into fibroblast-like cells with a spindle morphology. Additionally, their cell migratory speed was significantly increased from 8.3 ± 2.2 μm/h to 32.5 ± 4.7 μm/h (p = 0.02; Fig 1).

Bottom Line: Mesenchymal markers were seen in biliary epithelial cells (BECs), with collagen deposited around the bile duct.Expression of epithelial marker mRNAs and proteins in HIBECs decreased with prolonged cold preservation (CP), while mesenchymal marker expression increased.Expression of E-cadherin was increased in HIBECs following Trps1 adenovirus infection and CP/reperfusion injury (CPRI), with vimentin expression levels reduced and CPRI-mediated epithelial-mesenchymal transition (EMT) inhibited.

View Article: PubMed Central - PubMed

Affiliation: Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, No. 29 Gaotanyan Road, Shapingba District, Chongqing, 400038, China.

ABSTRACT

Objective: To investigate the role(s) of Trps1 in non-anastomotic biliary stricture (NABS) following liver transplantation.

Methods: Immunohistochemical and histological techniques were used to detect Trps1, E-cadherin, CK19, vimentin, α-SMA, and collagen deposition. Human intrahepatic biliary epithelial cells (HIBECs) were infected with a Trps1 adenovirus, or transfected with Trps1 short-interfering RNAs (siRNAs). Reverse transcription polymerase chain reaction (RT-PCR) assays and western blotting were used to determine expression levels of epithelial and mesenchymal markers, and Trps1 in HIBECs.

Results: Expression of Trps1 and epithelial markers was down-regulated or absent in NABS liver samples. Mesenchymal markers were seen in biliary epithelial cells (BECs), with collagen deposited around the bile duct. Trps1 expression positively correlated with epithelial markers. Expression of epithelial marker mRNAs and proteins in HIBECs decreased with prolonged cold preservation (CP), while mesenchymal marker expression increased. A 12-h CP period led to increased Trps1 mRNA and protein levels. Expression of E-cadherin was increased in HIBECs following Trps1 adenovirus infection and CP/reperfusion injury (CPRI), with vimentin expression levels reduced and CPRI-mediated epithelial-mesenchymal transition (EMT) inhibited. Transfection of HIBECs with Trps1 siRNAs in conjunction with CPRI revealed that E-cadherin expression was decreased, vimentin expression was increased, and CPRI-mediated EMT was promoted.

Conclusion: Trps1 is involved in NABS pathogenesis following liver transplantation and negatively correlates with BEC EMT and biliary fibrosis in liver grafts. Trps1 demonstrates antagonistic effects that could reverse EMT.

Show MeSH
Related in: MedlinePlus