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Characterization of the structure and expression of mouse Itpa gene and its related sequences in the mouse genome.

Behmanesh M, Sakumi K, Tsuchimoto D, Torisu K, Ohnishi-Honda Y, Rancourt DE, Nakabeppu Y - DNA Res. (2015)

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Expression of mouse Itpa gene in mouse tissue and its association with cell proliferation. A. The expression of Itpa mRNA in various types of adult mouse tissue. Total RNAs (20 μg each) extracted from various types of mouse tissue were electrophoresed, transferred onto a HybondTM-N+ nylon membrane, and subsequently probed with 32P-labeled fragment containing the entire coding region from the type A Itpa cDNA (731 bp) (top panel) and the 18S rRNA probe49 (middle panel), as previously described.27,50 The arrowhead indicates Itpa mRNA. In the bottom panel, the relative amounts of Itpa mRNA to 18S rRNA were calculated based on the radioactivity. The ratio of the relative amount of each transcript to that in the testis is shown. B. The expression of Itpa mRNA in quiescent and serum-stimulated BALB/c3T3 cells. The total RNA isolated was subjected to Northern blot analyses to determine the expression amount of Itpa mRNA and 18S rRNA. The data for 18S rRNA was originally published in Ide Y, Tsuchimoto D, Tominaga Y, Iwamoto Y, Nakabeppu Y, Genomics, 81, 47–57, 200327, © Elsevier, and the same blot was used to detect Itpa mRNA in the present study. C. The relative amounts of Itpa mRNA to 18S rRNA were calculated based on radioactivity. The ratio of the relative amount of each transcript to that in the quiescent cells is shown (closed circle). The percentages of the cells in the S phase of the cell cycle were determined by flow cytometry as previously described27,28 and then were plotted with a dotted line.
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DSV006F1: Expression of mouse Itpa gene in mouse tissue and its association with cell proliferation. A. The expression of Itpa mRNA in various types of adult mouse tissue. Total RNAs (20 μg each) extracted from various types of mouse tissue were electrophoresed, transferred onto a HybondTM-N+ nylon membrane, and subsequently probed with 32P-labeled fragment containing the entire coding region from the type A Itpa cDNA (731 bp) (top panel) and the 18S rRNA probe49 (middle panel), as previously described.27,50 The arrowhead indicates Itpa mRNA. In the bottom panel, the relative amounts of Itpa mRNA to 18S rRNA were calculated based on the radioactivity. The ratio of the relative amount of each transcript to that in the testis is shown. B. The expression of Itpa mRNA in quiescent and serum-stimulated BALB/c3T3 cells. The total RNA isolated was subjected to Northern blot analyses to determine the expression amount of Itpa mRNA and 18S rRNA. The data for 18S rRNA was originally published in Ide Y, Tsuchimoto D, Tominaga Y, Iwamoto Y, Nakabeppu Y, Genomics, 81, 47–57, 200327, © Elsevier, and the same blot was used to detect Itpa mRNA in the present study. C. The relative amounts of Itpa mRNA to 18S rRNA were calculated based on radioactivity. The ratio of the relative amount of each transcript to that in the quiescent cells is shown (closed circle). The percentages of the cells in the S phase of the cell cycle were determined by flow cytometry as previously described27,28 and then were plotted with a dotted line.

Mentions: The authors did not fully acknowledge the original publication when they reused part of a figure in their Fig. 7B in the above article. The legend for Figure 7 should read as follows. A new version of Fig. 7C is also given below as the labels on the x- and y-axes were not visible in the original published version. The authors would like to apologize for these errors.Figure 7.


Characterization of the structure and expression of mouse Itpa gene and its related sequences in the mouse genome.

Behmanesh M, Sakumi K, Tsuchimoto D, Torisu K, Ohnishi-Honda Y, Rancourt DE, Nakabeppu Y - DNA Res. (2015)

Expression of mouse Itpa gene in mouse tissue and its association with cell proliferation. A. The expression of Itpa mRNA in various types of adult mouse tissue. Total RNAs (20 μg each) extracted from various types of mouse tissue were electrophoresed, transferred onto a HybondTM-N+ nylon membrane, and subsequently probed with 32P-labeled fragment containing the entire coding region from the type A Itpa cDNA (731 bp) (top panel) and the 18S rRNA probe49 (middle panel), as previously described.27,50 The arrowhead indicates Itpa mRNA. In the bottom panel, the relative amounts of Itpa mRNA to 18S rRNA were calculated based on the radioactivity. The ratio of the relative amount of each transcript to that in the testis is shown. B. The expression of Itpa mRNA in quiescent and serum-stimulated BALB/c3T3 cells. The total RNA isolated was subjected to Northern blot analyses to determine the expression amount of Itpa mRNA and 18S rRNA. The data for 18S rRNA was originally published in Ide Y, Tsuchimoto D, Tominaga Y, Iwamoto Y, Nakabeppu Y, Genomics, 81, 47–57, 200327, © Elsevier, and the same blot was used to detect Itpa mRNA in the present study. C. The relative amounts of Itpa mRNA to 18S rRNA were calculated based on radioactivity. The ratio of the relative amount of each transcript to that in the quiescent cells is shown (closed circle). The percentages of the cells in the S phase of the cell cycle were determined by flow cytometry as previously described27,28 and then were plotted with a dotted line.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4401328&req=5

DSV006F1: Expression of mouse Itpa gene in mouse tissue and its association with cell proliferation. A. The expression of Itpa mRNA in various types of adult mouse tissue. Total RNAs (20 μg each) extracted from various types of mouse tissue were electrophoresed, transferred onto a HybondTM-N+ nylon membrane, and subsequently probed with 32P-labeled fragment containing the entire coding region from the type A Itpa cDNA (731 bp) (top panel) and the 18S rRNA probe49 (middle panel), as previously described.27,50 The arrowhead indicates Itpa mRNA. In the bottom panel, the relative amounts of Itpa mRNA to 18S rRNA were calculated based on the radioactivity. The ratio of the relative amount of each transcript to that in the testis is shown. B. The expression of Itpa mRNA in quiescent and serum-stimulated BALB/c3T3 cells. The total RNA isolated was subjected to Northern blot analyses to determine the expression amount of Itpa mRNA and 18S rRNA. The data for 18S rRNA was originally published in Ide Y, Tsuchimoto D, Tominaga Y, Iwamoto Y, Nakabeppu Y, Genomics, 81, 47–57, 200327, © Elsevier, and the same blot was used to detect Itpa mRNA in the present study. C. The relative amounts of Itpa mRNA to 18S rRNA were calculated based on radioactivity. The ratio of the relative amount of each transcript to that in the quiescent cells is shown (closed circle). The percentages of the cells in the S phase of the cell cycle were determined by flow cytometry as previously described27,28 and then were plotted with a dotted line.
Mentions: The authors did not fully acknowledge the original publication when they reused part of a figure in their Fig. 7B in the above article. The legend for Figure 7 should read as follows. A new version of Fig. 7C is also given below as the labels on the x- and y-axes were not visible in the original published version. The authors would like to apologize for these errors.Figure 7.

View Article: PubMed Central - PubMed

No MeSH data available.


Related in: MedlinePlus