Enrichment of an intraspecific genetic map of upland cotton by developing markers using parental RAD sequencing.
Bottom Line: A genetic map including 1,013 loci was constructed using these results and 506 loci previously published for this population.Here, the development of markers using parental RAD sequencing was effective, and a high-density intraspecific genetic map was constructed.This map can be used for molecular marker-assisted selection in cotton.
Affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China.Show MeSH
Mentions: A total of 14,433 contigs containing markers were used to analyse the distribution of the markers on the A2 and D5 genomes. We used all of the 14,433 contigs to BLAST with the sequences of the A2 and D5 genomes, and 14,103 (97.71%) contigs were matched (Fig. 7). Of the 14,103 contigs, 6,995 were matched on the 13 chromosomes of the A2 genome, and 7,108 were matched on the 13 chromosomes of the D5 genome. The hits were found in an essentially uniform distribution on every chromosome, except there were slightly fewer on the Ga2 and Ga5 of the A2 genome and on Gr3 and Gr12 of the D5 genome.Figure 7.
Affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China.