Enrichment of an intraspecific genetic map of upland cotton by developing markers using parental RAD sequencing.
Bottom Line: A genetic map including 1,013 loci was constructed using these results and 506 loci previously published for this population.Here, the development of markers using parental RAD sequencing was effective, and a high-density intraspecific genetic map was constructed.This map can be used for molecular marker-assisted selection in cotton.
Affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China.Show MeSH
Mentions: In all, 15,535 (6.17%) sequences of the Gh-D-J data set were significantly matched to 7,719 A. thaliana genes (Supplementary Table S1). The annotated Gh-D-J sequences were further functionally classified using a plant-specific GO slim (Fig. 5). Of the Gh-D-J sequences, 41,222 GO terms were categorized under biological process, 19,406 under cellular component, and 13,899 under molecular function. Fibre development is a very important process in cotton. Then 58 contigs were identified as being related to the MYB family, 10 contigs were found to be related to expansin biosynthesis, and 27 contigs were found to be related to the ethylene response. A total of 60 contigs were related to cell wall and cellulose biogenesis, 17 of which were related to cellulose synthase (CESA) genes (16 were required in expanding primary walls, and 1 was required in the thickening of secondary walls), 11 to the cellulose synthase protein family, and 32 to cellulose synthase-like (CSL) genes.Figure 5.
Affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China.