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Enrichment of an intraspecific genetic map of upland cotton by developing markers using parental RAD sequencing.

Wang H, Jin X, Zhang B, Shen C, Lin Z - DNA Res. (2015)

Bottom Line: A genetic map including 1,013 loci was constructed using these results and 506 loci previously published for this population.Here, the development of markers using parental RAD sequencing was effective, and a high-density intraspecific genetic map was constructed.This map can be used for molecular marker-assisted selection in cotton.

View Article: PubMed Central - PubMed

Affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China.

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Comparative analysis of the flanking sequences of markers of DH962 and Jimian5.
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DSU047F2: Comparative analysis of the flanking sequences of markers of DH962 and Jimian5.

Mentions: Among markers between DH962 and Jimian5, some were located on the same location or homologous location. We used the immediate flanking sequences (≥20 bp on both sides) of Jimian5 markers to BLAST the immediate flanking sequences (≥20 bp on both sides) of DH962 markers with an E-value cut-off of 1e−5 (Fig. 2).Figure 2.


Enrichment of an intraspecific genetic map of upland cotton by developing markers using parental RAD sequencing.

Wang H, Jin X, Zhang B, Shen C, Lin Z - DNA Res. (2015)

Comparative analysis of the flanking sequences of markers of DH962 and Jimian5.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4401325&req=5

DSU047F2: Comparative analysis of the flanking sequences of markers of DH962 and Jimian5.
Mentions: Among markers between DH962 and Jimian5, some were located on the same location or homologous location. We used the immediate flanking sequences (≥20 bp on both sides) of Jimian5 markers to BLAST the immediate flanking sequences (≥20 bp on both sides) of DH962 markers with an E-value cut-off of 1e−5 (Fig. 2).Figure 2.

Bottom Line: A genetic map including 1,013 loci was constructed using these results and 506 loci previously published for this population.Here, the development of markers using parental RAD sequencing was effective, and a high-density intraspecific genetic map was constructed.This map can be used for molecular marker-assisted selection in cotton.

View Article: PubMed Central - PubMed

Affiliation: National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, Hubei, China.

Show MeSH