Limits...
Determination of reference interval for presepsin, an early marker for sepsis.

Giavarina D, Carta M - Biochem Med (Zagreb) (2015)

Bottom Line: Overall, the reference limits for the presepsin were 55-184 pg/mL (90% confidence intervals, CI, were 45 to 58 and 161 to 214, respectively).There were no significant differences between males and females and the presepsin concentrations were not even particularly influenced by age.Reference values can help to distinguish and quickly rule out healthy subjects or patients with other pathologies.

View Article: PubMed Central - PubMed

Affiliation: Clinical Chemistry and Haematology Laboratory, San Bortolo Hospital, Vicenza, Italy.

ABSTRACT

Introduction: Presepsin, the circulating soluble form of CD14 subtype (sCD14-ST) is a new emerging early marker for sepsis. Various cutoff levels of presepsin have been proposed, to discriminate between systemic bacterial and nonbacterial infectious diseases. The aim of this work was to define the reference interval for presepsin according to the CLSI C28-A3c approved guideline.

Materials and methods: Reference individuals (N=200; 120 females) aged 18-75 years (median 39 years), free from inflammatory diseases, were selected for the study. Presepsin concentrations were measured by a commercially available chemiluminescent enzyme immunoassay (PATHFASTTM, Mitsubishi Chemical Europe GmbH, Düsseldorf, Germany). Reference limits were calculated using the non-parametric percentile method.

Results: Overall, the reference limits for the presepsin were 55-184 pg/mL (90% confidence intervals, CI, were 45 to 58 and 161 to 214, respectively). There were no significant differences between males and females and the presepsin concentrations were not even particularly influenced by age. The upper reference limit for the presepsin is much lower than every cut-off limit so far proposed, both for sepsis and also for systemic inflammatory response syndrome.

Conclusion: Specific decision levels are required to define the diagnostic and prognostic roles of presepsin in different settings of inflammatory and infectious diseases. Reference values can help to distinguish and quickly rule out healthy subjects or patients with other pathologies.

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Mentions: A total of 200 subjects were included in the study, 120 females and 80 males, aged 18-72 years and 18-75 years (median age 36 and 46 years), respectively. The reference limits for the presepsin were 55–184 pg/mL. The CI limits and the differences between female and male are summarized in table 1 and Figure 1. A relationship model between age and reference intervals is represented in figure 2. For ages 20, 30, 40, 50, 60 and 70 years the 0.025 and 0.975 centiles of estimated presepsin was 52-182, 49-165, 53-168, 63-176, 76-172, 85-143 pg/mL, respectively. A slight increase in the concentrations of presepsin is apparently associated with age. A reduction of inter-individual variability is observed after age 50, for both genders.


Determination of reference interval for presepsin, an early marker for sepsis.

Giavarina D, Carta M - Biochem Med (Zagreb) (2015)

© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4401310&req=5

Mentions: A total of 200 subjects were included in the study, 120 females and 80 males, aged 18-72 years and 18-75 years (median age 36 and 46 years), respectively. The reference limits for the presepsin were 55–184 pg/mL. The CI limits and the differences between female and male are summarized in table 1 and Figure 1. A relationship model between age and reference intervals is represented in figure 2. For ages 20, 30, 40, 50, 60 and 70 years the 0.025 and 0.975 centiles of estimated presepsin was 52-182, 49-165, 53-168, 63-176, 76-172, 85-143 pg/mL, respectively. A slight increase in the concentrations of presepsin is apparently associated with age. A reduction of inter-individual variability is observed after age 50, for both genders.

Bottom Line: Overall, the reference limits for the presepsin were 55-184 pg/mL (90% confidence intervals, CI, were 45 to 58 and 161 to 214, respectively).There were no significant differences between males and females and the presepsin concentrations were not even particularly influenced by age.Reference values can help to distinguish and quickly rule out healthy subjects or patients with other pathologies.

View Article: PubMed Central - PubMed

Affiliation: Clinical Chemistry and Haematology Laboratory, San Bortolo Hospital, Vicenza, Italy.

ABSTRACT

Introduction: Presepsin, the circulating soluble form of CD14 subtype (sCD14-ST) is a new emerging early marker for sepsis. Various cutoff levels of presepsin have been proposed, to discriminate between systemic bacterial and nonbacterial infectious diseases. The aim of this work was to define the reference interval for presepsin according to the CLSI C28-A3c approved guideline.

Materials and methods: Reference individuals (N=200; 120 females) aged 18-75 years (median 39 years), free from inflammatory diseases, were selected for the study. Presepsin concentrations were measured by a commercially available chemiluminescent enzyme immunoassay (PATHFASTTM, Mitsubishi Chemical Europe GmbH, Düsseldorf, Germany). Reference limits were calculated using the non-parametric percentile method.

Results: Overall, the reference limits for the presepsin were 55-184 pg/mL (90% confidence intervals, CI, were 45 to 58 and 161 to 214, respectively). There were no significant differences between males and females and the presepsin concentrations were not even particularly influenced by age. The upper reference limit for the presepsin is much lower than every cut-off limit so far proposed, both for sepsis and also for systemic inflammatory response syndrome.

Conclusion: Specific decision levels are required to define the diagnostic and prognostic roles of presepsin in different settings of inflammatory and infectious diseases. Reference values can help to distinguish and quickly rule out healthy subjects or patients with other pathologies.

Show MeSH
Related in: MedlinePlus