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Neurons produce FGF2 and VEGF and secrete them at least in part by shedding extracellular vesicles.

Schiera G, Proia P, Alberti C, Mineo M, Savettieri G, Di Liegro I - J. Cell. Mol. Med. (2007 Nov-Dec)

Bottom Line: We previously found that neurons are able to affect the ability of brain capillary endothelial cells to form in vitro a monolayer with properties resembling the blood-brain barrier.In the present paper, we report that neurons produce both vascular endothelial growth factor and fibroblast growth factor 2, two well-known angiogenic factors.Shedding of extracellular vesicles by neurons was also confirmed by scanner electron microscopy.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Scienze Biochimiche, Università degli Studi di Palermo, Palermo, Italy.

ABSTRACT
We previously found that neurons are able to affect the ability of brain capillary endothelial cells to form in vitro a monolayer with properties resembling the blood-brain barrier. We then looked, by immunofluorescence and western analysis, for factors, produced by neurons, with the potential to influence growth and differentiation of endothelial cells. In the present paper, we report that neurons produce both vascular endothelial growth factor and fibroblast growth factor 2, two well-known angiogenic factors. More interestingly, we gained evidence that both factors are released by neurons, at least in part, by shedding of extracellular vesicles, that contain beta1 integrin, a membrane protein already known to be part of extracellular vesicles released by tumour cells. Shedding of extracellular vesicles by neurons was also confirmed by scanner electron microscopy.

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Analysis by scanner electron microscopy of neuronal cultures. Cells produce and release extracellular vesicular structures (indicated by arrows). A detail (in the white box) of part A was observed at higher magnification (B).
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fig02: Analysis by scanner electron microscopy of neuronal cultures. Cells produce and release extracellular vesicular structures (indicated by arrows). A detail (in the white box) of part A was observed at higher magnification (B).

Mentions: The fact that neurons produce structures resembling MVs has been also confirmed by scanner electron microscopy (Fig. 2). As shown in Fig. 2B, some of these structures are very large, around 2000 nm (see below).


Neurons produce FGF2 and VEGF and secrete them at least in part by shedding extracellular vesicles.

Schiera G, Proia P, Alberti C, Mineo M, Savettieri G, Di Liegro I - J. Cell. Mol. Med. (2007 Nov-Dec)

Analysis by scanner electron microscopy of neuronal cultures. Cells produce and release extracellular vesicular structures (indicated by arrows). A detail (in the white box) of part A was observed at higher magnification (B).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4401300&req=5

fig02: Analysis by scanner electron microscopy of neuronal cultures. Cells produce and release extracellular vesicular structures (indicated by arrows). A detail (in the white box) of part A was observed at higher magnification (B).
Mentions: The fact that neurons produce structures resembling MVs has been also confirmed by scanner electron microscopy (Fig. 2). As shown in Fig. 2B, some of these structures are very large, around 2000 nm (see below).

Bottom Line: We previously found that neurons are able to affect the ability of brain capillary endothelial cells to form in vitro a monolayer with properties resembling the blood-brain barrier.In the present paper, we report that neurons produce both vascular endothelial growth factor and fibroblast growth factor 2, two well-known angiogenic factors.Shedding of extracellular vesicles by neurons was also confirmed by scanner electron microscopy.

View Article: PubMed Central - PubMed

Affiliation: Dipartimento di Scienze Biochimiche, Università degli Studi di Palermo, Palermo, Italy.

ABSTRACT
We previously found that neurons are able to affect the ability of brain capillary endothelial cells to form in vitro a monolayer with properties resembling the blood-brain barrier. We then looked, by immunofluorescence and western analysis, for factors, produced by neurons, with the potential to influence growth and differentiation of endothelial cells. In the present paper, we report that neurons produce both vascular endothelial growth factor and fibroblast growth factor 2, two well-known angiogenic factors. More interestingly, we gained evidence that both factors are released by neurons, at least in part, by shedding of extracellular vesicles, that contain beta1 integrin, a membrane protein already known to be part of extracellular vesicles released by tumour cells. Shedding of extracellular vesicles by neurons was also confirmed by scanner electron microscopy.

Show MeSH
Related in: MedlinePlus