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Electron microscope tomography: further demonstration of nanocontacts between caveolae and smooth muscle sarcoplasmic reticulum.

Gherghiceanu M, Popescu LM - J. Cell. Mol. Med. (2007 Nov-Dec)

Bottom Line: A spatial relationship between caveolae and sarcoplasmic reticulum (SR) in smooth muscle cells (SMC) was previously reported in computer-assisted three-dimensional reconstruction from transmission electron microscope serial sections.Cellular tomography using transmission electron microscopy tomography (EMT) is the only available technology to reliably chart the inside of a cell and is therefore an essential technology in the study of organellar nanospatial relationships.Using EMT we further demonstrate here that caveolae and peripheral SR in visceral SMC build constantly spatial units, presumably responsible for a vectorial control of free Ca2+ cytoplasmic concentrations in definite nanospaces.

View Article: PubMed Central - PubMed

Affiliation: Victor Babes National Institute of Pathology, Bucharest, Romania.

ABSTRACT
A spatial relationship between caveolae and sarcoplasmic reticulum (SR) in smooth muscle cells (SMC) was previously reported in computer-assisted three-dimensional reconstruction from transmission electron microscope serial sections. The knowledge of the three-dimensional organization of the cortical space of SMC is essential to understand caveolae function at the cellular level. Cellular tomography using transmission electron microscopy tomography (EMT) is the only available technology to reliably chart the inside of a cell and is therefore an essential technology in the study of organellar nanospatial relationships. Using EMT we further demonstrate here that caveolae and peripheral SR in visceral SMC build constantly spatial units, presumably responsible for a vectorial control of free Ca2+ cytoplasmic concentrations in definite nanospaces.

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Related in: MedlinePlus

The 3D reconstructed volume of smooth muscle cells (SMC) obtained by electron tomography and digitally colored in red is rotated with 90°. A, C illustrate the thickness of section which is 500 nm (scale bar = 0.5 μm). In the front (B) and back (D) views could be seen tissue features: SMC with a crowded cytoplasm and mitochondria (m) and a bunch of collagen fibers.
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fig01: The 3D reconstructed volume of smooth muscle cells (SMC) obtained by electron tomography and digitally colored in red is rotated with 90°. A, C illustrate the thickness of section which is 500 nm (scale bar = 0.5 μm). In the front (B) and back (D) views could be seen tissue features: SMC with a crowded cytoplasm and mitochondria (m) and a bunch of collagen fibers.

Mentions: EMT emphasized tremendously complex contractile system of SMC (Fig. 1 and on-line supplement) which appear as ‘background noise’ in a classical picture from transmission electron microscopy (Fig. 2). Focusing on caveolar domains (Fig. 3), EMT showed that each caveolae have at least one contact point with SR. Our previously results, particularly the 3D reconstructions from serial ultrathin sections highlight the idea that caveolae and SR form a unique feature, cortical continuum compartment in SMC. Cellular electron tomography of the cortical space of SMC augments the idea of a structural unit formed by caveolae and SR which could be regarded as a ‘super-Ca2+ release/storage unit’.


Electron microscope tomography: further demonstration of nanocontacts between caveolae and smooth muscle sarcoplasmic reticulum.

Gherghiceanu M, Popescu LM - J. Cell. Mol. Med. (2007 Nov-Dec)

The 3D reconstructed volume of smooth muscle cells (SMC) obtained by electron tomography and digitally colored in red is rotated with 90°. A, C illustrate the thickness of section which is 500 nm (scale bar = 0.5 μm). In the front (B) and back (D) views could be seen tissue features: SMC with a crowded cytoplasm and mitochondria (m) and a bunch of collagen fibers.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4401299&req=5

fig01: The 3D reconstructed volume of smooth muscle cells (SMC) obtained by electron tomography and digitally colored in red is rotated with 90°. A, C illustrate the thickness of section which is 500 nm (scale bar = 0.5 μm). In the front (B) and back (D) views could be seen tissue features: SMC with a crowded cytoplasm and mitochondria (m) and a bunch of collagen fibers.
Mentions: EMT emphasized tremendously complex contractile system of SMC (Fig. 1 and on-line supplement) which appear as ‘background noise’ in a classical picture from transmission electron microscopy (Fig. 2). Focusing on caveolar domains (Fig. 3), EMT showed that each caveolae have at least one contact point with SR. Our previously results, particularly the 3D reconstructions from serial ultrathin sections highlight the idea that caveolae and SR form a unique feature, cortical continuum compartment in SMC. Cellular electron tomography of the cortical space of SMC augments the idea of a structural unit formed by caveolae and SR which could be regarded as a ‘super-Ca2+ release/storage unit’.

Bottom Line: A spatial relationship between caveolae and sarcoplasmic reticulum (SR) in smooth muscle cells (SMC) was previously reported in computer-assisted three-dimensional reconstruction from transmission electron microscope serial sections.Cellular tomography using transmission electron microscopy tomography (EMT) is the only available technology to reliably chart the inside of a cell and is therefore an essential technology in the study of organellar nanospatial relationships.Using EMT we further demonstrate here that caveolae and peripheral SR in visceral SMC build constantly spatial units, presumably responsible for a vectorial control of free Ca2+ cytoplasmic concentrations in definite nanospaces.

View Article: PubMed Central - PubMed

Affiliation: Victor Babes National Institute of Pathology, Bucharest, Romania.

ABSTRACT
A spatial relationship between caveolae and sarcoplasmic reticulum (SR) in smooth muscle cells (SMC) was previously reported in computer-assisted three-dimensional reconstruction from transmission electron microscope serial sections. The knowledge of the three-dimensional organization of the cortical space of SMC is essential to understand caveolae function at the cellular level. Cellular tomography using transmission electron microscopy tomography (EMT) is the only available technology to reliably chart the inside of a cell and is therefore an essential technology in the study of organellar nanospatial relationships. Using EMT we further demonstrate here that caveolae and peripheral SR in visceral SMC build constantly spatial units, presumably responsible for a vectorial control of free Ca2+ cytoplasmic concentrations in definite nanospaces.

Show MeSH
Related in: MedlinePlus