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Differential cytokine activity and morphology during wound healing in the neonatal and adult rat skin.

Wagner W, Wehrmann M - J. Cell. Mol. Med. (2007 Nov-Dec)

Bottom Line: Cytokines are known to play a key role in this process.Expression typically peaked between 12 hrs and 3 days post-wounding, and was not detectable any more at days 10 and 30.The neonate specimen showed more rapid re-epithelialization, far less inflammation and scarring, and larger restitution of original tissue architecture than their adult counterparts, resembling a prenatal healing pattern.

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology, University of Tübingen, Tübingen, Germany. w.wagner@med.uni-tuebingen.de

ABSTRACT
Wound-healing mechanisms change during transition from prenatal to postnatal stage. Cytokines are known to play a key role in this process. The current study investigated the differential cytokine activity and healing morphology during healing of incisional skin wounds in rats of the ages neonatal (p0), 3 days old (p3) and adult, after six different healing times (2 hrs to 30 days). All seven tested cytokines (Transforming Growth Factor (TGF) alpha, TGFbeta1, -beta2 and -beta3, IGF 1, Platelet Derived Growth Factor A (PDGF A), basic Fibroblast Growth Factor (bFGF) exhibited higher expression in the adult wounds than at the ages p0 and p3. Expression typically peaked between 12 hrs and 3 days post-wounding, and was not detectable any more at days 10 and 30. The neonate specimen showed more rapid re-epithelialization, far less inflammation and scarring, and larger restitution of original tissue architecture than their adult counterparts, resembling a prenatal healing pattern. The results may encourage the use of neonatal rat skin as a wound-healing model for further studies, instead of the more complicated prenatal animal models. Secondly, the data may recommend inhibition of PDGF A, basic FGF or TGF-beta1 as therapeutic targets in efforts to optimize wound healing in the adult organism.

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Temporal distribution of cytokine expression in the wound site. Semi-quantitative evaluation by age group. Lines: TGF-α: —x—; TGF-β1: …□…; TGF-β2: ––O;––; TGF-β3: ––*––; PDGF-A: ––+––; IGF-1: —-▵—-; bFGF: – . –·–▿–·–.
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fig04: Temporal distribution of cytokine expression in the wound site. Semi-quantitative evaluation by age group. Lines: TGF-α: —x—; TGF-β1: …□…; TGF-β2: ––O;––; TGF-β3: ––*––; PDGF-A: ––+––; IGF-1: —-▵—-; bFGF: – . –·–▿–·–.

Mentions: As an overall result, expression of all seven investigated cytokines was distinctly higher in the area of the lesion in the adult animals than at the ages p0 and p3 (Fig. 2 shows expression of TGF-α as example). P0 and p3 did not differ much in their paucity of cytokine expression. The only significant expressions in p0 and p3 were the following: low (to medium) expression of TGF-α in p0 and p3 at 12 hrs, 24 hrs and 3 days p.w., low expression of IGF 1 in p0 at 3 days p.w., low expression of TGF-β2 in p3 at 3 and 10 days p.w., and low expression of IGF 1 in p3 at 12 hrs and 10 days p.w.. However, expression was higher in all corresponding adult specimen (Figs. 3 and 4).


Differential cytokine activity and morphology during wound healing in the neonatal and adult rat skin.

Wagner W, Wehrmann M - J. Cell. Mol. Med. (2007 Nov-Dec)

Temporal distribution of cytokine expression in the wound site. Semi-quantitative evaluation by age group. Lines: TGF-α: —x—; TGF-β1: …□…; TGF-β2: ––O;––; TGF-β3: ––*––; PDGF-A: ––+––; IGF-1: —-▵—-; bFGF: – . –·–▿–·–.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4401296&req=5

fig04: Temporal distribution of cytokine expression in the wound site. Semi-quantitative evaluation by age group. Lines: TGF-α: —x—; TGF-β1: …□…; TGF-β2: ––O;––; TGF-β3: ––*––; PDGF-A: ––+––; IGF-1: —-▵—-; bFGF: – . –·–▿–·–.
Mentions: As an overall result, expression of all seven investigated cytokines was distinctly higher in the area of the lesion in the adult animals than at the ages p0 and p3 (Fig. 2 shows expression of TGF-α as example). P0 and p3 did not differ much in their paucity of cytokine expression. The only significant expressions in p0 and p3 were the following: low (to medium) expression of TGF-α in p0 and p3 at 12 hrs, 24 hrs and 3 days p.w., low expression of IGF 1 in p0 at 3 days p.w., low expression of TGF-β2 in p3 at 3 and 10 days p.w., and low expression of IGF 1 in p3 at 12 hrs and 10 days p.w.. However, expression was higher in all corresponding adult specimen (Figs. 3 and 4).

Bottom Line: Cytokines are known to play a key role in this process.Expression typically peaked between 12 hrs and 3 days post-wounding, and was not detectable any more at days 10 and 30.The neonate specimen showed more rapid re-epithelialization, far less inflammation and scarring, and larger restitution of original tissue architecture than their adult counterparts, resembling a prenatal healing pattern.

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology, University of Tübingen, Tübingen, Germany. w.wagner@med.uni-tuebingen.de

ABSTRACT
Wound-healing mechanisms change during transition from prenatal to postnatal stage. Cytokines are known to play a key role in this process. The current study investigated the differential cytokine activity and healing morphology during healing of incisional skin wounds in rats of the ages neonatal (p0), 3 days old (p3) and adult, after six different healing times (2 hrs to 30 days). All seven tested cytokines (Transforming Growth Factor (TGF) alpha, TGFbeta1, -beta2 and -beta3, IGF 1, Platelet Derived Growth Factor A (PDGF A), basic Fibroblast Growth Factor (bFGF) exhibited higher expression in the adult wounds than at the ages p0 and p3. Expression typically peaked between 12 hrs and 3 days post-wounding, and was not detectable any more at days 10 and 30. The neonate specimen showed more rapid re-epithelialization, far less inflammation and scarring, and larger restitution of original tissue architecture than their adult counterparts, resembling a prenatal healing pattern. The results may encourage the use of neonatal rat skin as a wound-healing model for further studies, instead of the more complicated prenatal animal models. Secondly, the data may recommend inhibition of PDGF A, basic FGF or TGF-beta1 as therapeutic targets in efforts to optimize wound healing in the adult organism.

Show MeSH
Related in: MedlinePlus