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Differential cytokine activity and morphology during wound healing in the neonatal and adult rat skin.

Wagner W, Wehrmann M - J. Cell. Mol. Med. (2007 Nov-Dec)

Bottom Line: Cytokines are known to play a key role in this process.Expression typically peaked between 12 hrs and 3 days post-wounding, and was not detectable any more at days 10 and 30.The neonate specimen showed more rapid re-epithelialization, far less inflammation and scarring, and larger restitution of original tissue architecture than their adult counterparts, resembling a prenatal healing pattern.

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology, University of Tübingen, Tübingen, Germany. w.wagner@med.uni-tuebingen.de

ABSTRACT
Wound-healing mechanisms change during transition from prenatal to postnatal stage. Cytokines are known to play a key role in this process. The current study investigated the differential cytokine activity and healing morphology during healing of incisional skin wounds in rats of the ages neonatal (p0), 3 days old (p3) and adult, after six different healing times (2 hrs to 30 days). All seven tested cytokines (Transforming Growth Factor (TGF) alpha, TGFbeta1, -beta2 and -beta3, IGF 1, Platelet Derived Growth Factor A (PDGF A), basic Fibroblast Growth Factor (bFGF) exhibited higher expression in the adult wounds than at the ages p0 and p3. Expression typically peaked between 12 hrs and 3 days post-wounding, and was not detectable any more at days 10 and 30. The neonate specimen showed more rapid re-epithelialization, far less inflammation and scarring, and larger restitution of original tissue architecture than their adult counterparts, resembling a prenatal healing pattern. The results may encourage the use of neonatal rat skin as a wound-healing model for further studies, instead of the more complicated prenatal animal models. Secondly, the data may recommend inhibition of PDGF A, basic FGF or TGF-beta1 as therapeutic targets in efforts to optimize wound healing in the adult organism.

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Incisional wound area after six different healing times (2 hrs, 12 hrs, 24 hrs, 1 day, 3 days, 10 days, 30 days) in neonatal, 3 day old and adult rat skin. Haematoxylin-Eosin-stain. Bar in left top = 200 μm (p0 and p3 at 2 hrs, 12 hrs, 24 hrs and 3 days) or 500 μm (p0 and p3 at 10 days and 30 days, all adult sections).
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fig01: Incisional wound area after six different healing times (2 hrs, 12 hrs, 24 hrs, 1 day, 3 days, 10 days, 30 days) in neonatal, 3 day old and adult rat skin. Haematoxylin-Eosin-stain. Bar in left top = 200 μm (p0 and p3 at 2 hrs, 12 hrs, 24 hrs and 3 days) or 500 μm (p0 and p3 at 10 days and 30 days, all adult sections).

Mentions: Two-hr post-wounding (p.w.), incisional gaps filled with blood and fibrin were seen in all age groups (Fig. 1). The wounds gaped wider in the p0- and p3-animals than in the adults. There was only little cellular activity perceivable at 2 hrs (immigration of sparse granulocytes in p3 and adult). Beginning immigration of lymphocytes to the wound site was observed 24 hrs p.w. in the p0- and p3-specimen, however, the granulocytes still being predominant. In comparison, presence of granulocytes was lower in the adult stage at that time point, with incomplete infiltration of the fibrin-clots. In general, an inflammatory reaction characterized by immigration of granulocytes, makrophagen und few lymphocytes was observed both at ages p0 and adult, however, that inflammation lasted distinctively shorter at p0 than at adult (ca. 3 days versus ca. 30 days).


Differential cytokine activity and morphology during wound healing in the neonatal and adult rat skin.

Wagner W, Wehrmann M - J. Cell. Mol. Med. (2007 Nov-Dec)

Incisional wound area after six different healing times (2 hrs, 12 hrs, 24 hrs, 1 day, 3 days, 10 days, 30 days) in neonatal, 3 day old and adult rat skin. Haematoxylin-Eosin-stain. Bar in left top = 200 μm (p0 and p3 at 2 hrs, 12 hrs, 24 hrs and 3 days) or 500 μm (p0 and p3 at 10 days and 30 days, all adult sections).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4401296&req=5

fig01: Incisional wound area after six different healing times (2 hrs, 12 hrs, 24 hrs, 1 day, 3 days, 10 days, 30 days) in neonatal, 3 day old and adult rat skin. Haematoxylin-Eosin-stain. Bar in left top = 200 μm (p0 and p3 at 2 hrs, 12 hrs, 24 hrs and 3 days) or 500 μm (p0 and p3 at 10 days and 30 days, all adult sections).
Mentions: Two-hr post-wounding (p.w.), incisional gaps filled with blood and fibrin were seen in all age groups (Fig. 1). The wounds gaped wider in the p0- and p3-animals than in the adults. There was only little cellular activity perceivable at 2 hrs (immigration of sparse granulocytes in p3 and adult). Beginning immigration of lymphocytes to the wound site was observed 24 hrs p.w. in the p0- and p3-specimen, however, the granulocytes still being predominant. In comparison, presence of granulocytes was lower in the adult stage at that time point, with incomplete infiltration of the fibrin-clots. In general, an inflammatory reaction characterized by immigration of granulocytes, makrophagen und few lymphocytes was observed both at ages p0 and adult, however, that inflammation lasted distinctively shorter at p0 than at adult (ca. 3 days versus ca. 30 days).

Bottom Line: Cytokines are known to play a key role in this process.Expression typically peaked between 12 hrs and 3 days post-wounding, and was not detectable any more at days 10 and 30.The neonate specimen showed more rapid re-epithelialization, far less inflammation and scarring, and larger restitution of original tissue architecture than their adult counterparts, resembling a prenatal healing pattern.

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology, University of Tübingen, Tübingen, Germany. w.wagner@med.uni-tuebingen.de

ABSTRACT
Wound-healing mechanisms change during transition from prenatal to postnatal stage. Cytokines are known to play a key role in this process. The current study investigated the differential cytokine activity and healing morphology during healing of incisional skin wounds in rats of the ages neonatal (p0), 3 days old (p3) and adult, after six different healing times (2 hrs to 30 days). All seven tested cytokines (Transforming Growth Factor (TGF) alpha, TGFbeta1, -beta2 and -beta3, IGF 1, Platelet Derived Growth Factor A (PDGF A), basic Fibroblast Growth Factor (bFGF) exhibited higher expression in the adult wounds than at the ages p0 and p3. Expression typically peaked between 12 hrs and 3 days post-wounding, and was not detectable any more at days 10 and 30. The neonate specimen showed more rapid re-epithelialization, far less inflammation and scarring, and larger restitution of original tissue architecture than their adult counterparts, resembling a prenatal healing pattern. The results may encourage the use of neonatal rat skin as a wound-healing model for further studies, instead of the more complicated prenatal animal models. Secondly, the data may recommend inhibition of PDGF A, basic FGF or TGF-beta1 as therapeutic targets in efforts to optimize wound healing in the adult organism.

Show MeSH
Related in: MedlinePlus