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Myocardial interstitial Cajal-like cells (ICLC) and their nanostructural relationships with intercalated discs: shed vesicles as intermediates.

Mandache E, Popescu LM, Gherghiceanu M - J. Cell. Mol. Med. (2007 Sep-Oct)

Bottom Line: Typically, ICLC have several very long, moniliform, cytoplasmic processes which establish closed contacts with nerve fibres, as well as each other.In the last case, electron-dense repetitive nanostructures ('pillars') (35-40 nm high and 100-150 nm wide, similar to adhesion molecules) fasten the ICLC to the myocytes.All these features suggest a juxtacrine and/or paracrine intercellular mutual modulation of ICLC and cardiomyocytes in the microenvironment of ID, possibly monitoring the cardiac functions, particularly the electrical activity.

View Article: PubMed Central - PubMed

Affiliation: Victor Babeçs National Institute of Pathology, Bucharest, Romania.

ABSTRACT
Intercalated discs (ID) are complex junctional units that connect cardiac myocytes mechanically and electrochemically. However, there is limited information concerning the cardiomyocyte interaction with interstitial non-muscle cells. Our previous studies showed that myocardial interstitial Cajal-like cells (ICLC) are located in between cardiomyocytes, blood capillaries and nerve fibres. Typically, ICLC have several very long, moniliform, cytoplasmic processes which establish closed contacts with nerve fibres, as well as each other. We report here ultrastructural evidence concerning the relationships of ICLC processes with ID. The ICLC cytoplasmic prolongations (tens micrometers length) preferentially pass by or stop nearby the ID. Transmission electron microscopy emphasized three distinct connecting features between the tips of ICLC extensions and myocytes at the 'mouth' of ID: free or budding shed vesicles, exocytotic multi-vesicular bodies and direct contacts. In the last case, electron-dense repetitive nanostructures ('pillars') (35-40 nm high and 100-150 nm wide, similar to adhesion molecules) fasten the ICLC to the myocytes. All these features suggest a juxtacrine and/or paracrine intercellular mutual modulation of ICLC and cardiomyocytes in the microenvironment of ID, possibly monitoring the cardiac functions, particularly the electrical activity.

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Related in: MedlinePlus

ICLC cytoplasmic extension in apposition with a myocyte in the intercalated discs (ID) area. The inset shows in an oblique-sectioned segment of plasma membrane (pm), four electron-dense attachment structures connecting the basal lamina (bl) of cardiomyocyte (M1) with the ICLC cytoplasmic process. Scale bar = 0.2 μm.
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fig08: ICLC cytoplasmic extension in apposition with a myocyte in the intercalated discs (ID) area. The inset shows in an oblique-sectioned segment of plasma membrane (pm), four electron-dense attachment structures connecting the basal lamina (bl) of cardiomyocyte (M1) with the ICLC cytoplasmic process. Scale bar = 0.2 μm.

Mentions: Moreover, the ultrastructural analysis showed electron-dense structures connecting the ICLC cytoplasmic processes by cardiomyocytes on the ID areas (Figs. 7 and 8). These anchoring structures (‘pillars’) have 35–40 nm high and 100–150 nm wide and show a repetitive pattern.


Myocardial interstitial Cajal-like cells (ICLC) and their nanostructural relationships with intercalated discs: shed vesicles as intermediates.

Mandache E, Popescu LM, Gherghiceanu M - J. Cell. Mol. Med. (2007 Sep-Oct)

ICLC cytoplasmic extension in apposition with a myocyte in the intercalated discs (ID) area. The inset shows in an oblique-sectioned segment of plasma membrane (pm), four electron-dense attachment structures connecting the basal lamina (bl) of cardiomyocyte (M1) with the ICLC cytoplasmic process. Scale bar = 0.2 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4401282&req=5

fig08: ICLC cytoplasmic extension in apposition with a myocyte in the intercalated discs (ID) area. The inset shows in an oblique-sectioned segment of plasma membrane (pm), four electron-dense attachment structures connecting the basal lamina (bl) of cardiomyocyte (M1) with the ICLC cytoplasmic process. Scale bar = 0.2 μm.
Mentions: Moreover, the ultrastructural analysis showed electron-dense structures connecting the ICLC cytoplasmic processes by cardiomyocytes on the ID areas (Figs. 7 and 8). These anchoring structures (‘pillars’) have 35–40 nm high and 100–150 nm wide and show a repetitive pattern.

Bottom Line: Typically, ICLC have several very long, moniliform, cytoplasmic processes which establish closed contacts with nerve fibres, as well as each other.In the last case, electron-dense repetitive nanostructures ('pillars') (35-40 nm high and 100-150 nm wide, similar to adhesion molecules) fasten the ICLC to the myocytes.All these features suggest a juxtacrine and/or paracrine intercellular mutual modulation of ICLC and cardiomyocytes in the microenvironment of ID, possibly monitoring the cardiac functions, particularly the electrical activity.

View Article: PubMed Central - PubMed

Affiliation: Victor Babeçs National Institute of Pathology, Bucharest, Romania.

ABSTRACT
Intercalated discs (ID) are complex junctional units that connect cardiac myocytes mechanically and electrochemically. However, there is limited information concerning the cardiomyocyte interaction with interstitial non-muscle cells. Our previous studies showed that myocardial interstitial Cajal-like cells (ICLC) are located in between cardiomyocytes, blood capillaries and nerve fibres. Typically, ICLC have several very long, moniliform, cytoplasmic processes which establish closed contacts with nerve fibres, as well as each other. We report here ultrastructural evidence concerning the relationships of ICLC processes with ID. The ICLC cytoplasmic prolongations (tens micrometers length) preferentially pass by or stop nearby the ID. Transmission electron microscopy emphasized three distinct connecting features between the tips of ICLC extensions and myocytes at the 'mouth' of ID: free or budding shed vesicles, exocytotic multi-vesicular bodies and direct contacts. In the last case, electron-dense repetitive nanostructures ('pillars') (35-40 nm high and 100-150 nm wide, similar to adhesion molecules) fasten the ICLC to the myocytes. All these features suggest a juxtacrine and/or paracrine intercellular mutual modulation of ICLC and cardiomyocytes in the microenvironment of ID, possibly monitoring the cardiac functions, particularly the electrical activity.

Show MeSH
Related in: MedlinePlus