Limits...
Myocardial interstitial Cajal-like cells (ICLC) and their nanostructural relationships with intercalated discs: shed vesicles as intermediates.

Mandache E, Popescu LM, Gherghiceanu M - J. Cell. Mol. Med. (2007 Sep-Oct)

Bottom Line: Typically, ICLC have several very long, moniliform, cytoplasmic processes which establish closed contacts with nerve fibres, as well as each other.In the last case, electron-dense repetitive nanostructures ('pillars') (35-40 nm high and 100-150 nm wide, similar to adhesion molecules) fasten the ICLC to the myocytes.All these features suggest a juxtacrine and/or paracrine intercellular mutual modulation of ICLC and cardiomyocytes in the microenvironment of ID, possibly monitoring the cardiac functions, particularly the electrical activity.

View Article: PubMed Central - PubMed

Affiliation: Victor Babeçs National Institute of Pathology, Bucharest, Romania.

ABSTRACT
Intercalated discs (ID) are complex junctional units that connect cardiac myocytes mechanically and electrochemically. However, there is limited information concerning the cardiomyocyte interaction with interstitial non-muscle cells. Our previous studies showed that myocardial interstitial Cajal-like cells (ICLC) are located in between cardiomyocytes, blood capillaries and nerve fibres. Typically, ICLC have several very long, moniliform, cytoplasmic processes which establish closed contacts with nerve fibres, as well as each other. We report here ultrastructural evidence concerning the relationships of ICLC processes with ID. The ICLC cytoplasmic prolongations (tens micrometers length) preferentially pass by or stop nearby the ID. Transmission electron microscopy emphasized three distinct connecting features between the tips of ICLC extensions and myocytes at the 'mouth' of ID: free or budding shed vesicles, exocytotic multi-vesicular bodies and direct contacts. In the last case, electron-dense repetitive nanostructures ('pillars') (35-40 nm high and 100-150 nm wide, similar to adhesion molecules) fasten the ICLC to the myocytes. All these features suggest a juxtacrine and/or paracrine intercellular mutual modulation of ICLC and cardiomyocytes in the microenvironment of ID, possibly monitoring the cardiac functions, particularly the electrical activity.

Show MeSH

Related in: MedlinePlus

An ICLC process ending in a myocytic pocket of myocardocyte M1 at the level of the intercalated disc (ID). Small electron-dense particles (50 nm or less) can be seen in the basal lamina thickness of the myocardocytes (arrows) or beneath plasma membrane in the cytoplasm of the myocardocyte M1 (arrowheads). Scale bar = 0.2 μm.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4401282&req=5

fig03: An ICLC process ending in a myocytic pocket of myocardocyte M1 at the level of the intercalated disc (ID). Small electron-dense particles (50 nm or less) can be seen in the basal lamina thickness of the myocardocytes (arrows) or beneath plasma membrane in the cytoplasm of the myocardocyte M1 (arrowheads). Scale bar = 0.2 μm.

Mentions: The transmission electron microscopy (TEM) investigation showed a strong affinity of ICLC for the neighbouring area of ID. The tips of ICLC cytoplasmic processes have been observed in proximity of about 55% of ID (Figs. 1–8). Most of the ICLC processes end up in the extracellular myocyte pockets associated with the ‘mouth'of ID (Figs. 1 and 3).


Myocardial interstitial Cajal-like cells (ICLC) and their nanostructural relationships with intercalated discs: shed vesicles as intermediates.

Mandache E, Popescu LM, Gherghiceanu M - J. Cell. Mol. Med. (2007 Sep-Oct)

An ICLC process ending in a myocytic pocket of myocardocyte M1 at the level of the intercalated disc (ID). Small electron-dense particles (50 nm or less) can be seen in the basal lamina thickness of the myocardocytes (arrows) or beneath plasma membrane in the cytoplasm of the myocardocyte M1 (arrowheads). Scale bar = 0.2 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4401282&req=5

fig03: An ICLC process ending in a myocytic pocket of myocardocyte M1 at the level of the intercalated disc (ID). Small electron-dense particles (50 nm or less) can be seen in the basal lamina thickness of the myocardocytes (arrows) or beneath plasma membrane in the cytoplasm of the myocardocyte M1 (arrowheads). Scale bar = 0.2 μm.
Mentions: The transmission electron microscopy (TEM) investigation showed a strong affinity of ICLC for the neighbouring area of ID. The tips of ICLC cytoplasmic processes have been observed in proximity of about 55% of ID (Figs. 1–8). Most of the ICLC processes end up in the extracellular myocyte pockets associated with the ‘mouth'of ID (Figs. 1 and 3).

Bottom Line: Typically, ICLC have several very long, moniliform, cytoplasmic processes which establish closed contacts with nerve fibres, as well as each other.In the last case, electron-dense repetitive nanostructures ('pillars') (35-40 nm high and 100-150 nm wide, similar to adhesion molecules) fasten the ICLC to the myocytes.All these features suggest a juxtacrine and/or paracrine intercellular mutual modulation of ICLC and cardiomyocytes in the microenvironment of ID, possibly monitoring the cardiac functions, particularly the electrical activity.

View Article: PubMed Central - PubMed

Affiliation: Victor Babeçs National Institute of Pathology, Bucharest, Romania.

ABSTRACT
Intercalated discs (ID) are complex junctional units that connect cardiac myocytes mechanically and electrochemically. However, there is limited information concerning the cardiomyocyte interaction with interstitial non-muscle cells. Our previous studies showed that myocardial interstitial Cajal-like cells (ICLC) are located in between cardiomyocytes, blood capillaries and nerve fibres. Typically, ICLC have several very long, moniliform, cytoplasmic processes which establish closed contacts with nerve fibres, as well as each other. We report here ultrastructural evidence concerning the relationships of ICLC processes with ID. The ICLC cytoplasmic prolongations (tens micrometers length) preferentially pass by or stop nearby the ID. Transmission electron microscopy emphasized three distinct connecting features between the tips of ICLC extensions and myocytes at the 'mouth' of ID: free or budding shed vesicles, exocytotic multi-vesicular bodies and direct contacts. In the last case, electron-dense repetitive nanostructures ('pillars') (35-40 nm high and 100-150 nm wide, similar to adhesion molecules) fasten the ICLC to the myocytes. All these features suggest a juxtacrine and/or paracrine intercellular mutual modulation of ICLC and cardiomyocytes in the microenvironment of ID, possibly monitoring the cardiac functions, particularly the electrical activity.

Show MeSH
Related in: MedlinePlus