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Matrix metalloproteinase-2, caveolins, focal adhesion kinase and c-Kit in cells of the mouse myocardium.

Cho WJ, Chow AK, Schulz R, Daniel EE - J. Cell. Mol. Med. (2007 Sep-Oct)

Bottom Line: In Cav-1(-/-) LV Cav-1 and MMP-2 were absent or reduced everywhere.To conclude, MMP-2 is closely associated with caveolins at FB and CEC as well as at CM.Also, MMP-2 is closely associated with FAK at FB and c-Kit at ICLC.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada.

ABSTRACT
Matrix metalloproteinase-2 (MMP-2) may play roles at intracellular and extracellular sites of the heart in ischaemia/reperfusion injury. Caveolins (Cav-1, -2 and -3) are lipid raft proteins which play roles in cell sig-nalling. This study examined, using immunohistochemistry and two photon confocal microscopy, if MMP-2 and caveolins co-localize at the plasma membrane of cardiac cells: cardiomyocytes (CM), fibroblasts (FB) and capillary endothelial cells (CEC) in the left ventricle (LV) of the Cav-1(+/+) and Cav-1(-/-) mouse heart. In Cav-1(+/+) mouse LV MMP-2 and Cav-1 co-localized at CM plasma membranes, and at multiple locations in FB and CEC. MMP-2 co-localized with Cav-2 only at CEC. MMP-2 co-localized with Cav-3 at CM plasma membranes and Z-lines, and partially at FB and CEC. In Cav-1(-/-) LV Cav-1 and MMP-2 were absent or reduced everywhere. Cav-2 appeared at CEC despite the absence of Cav-1. Cav-3 appeared at CM plasma membranes and Z-lines, FB and CEC. Also, FAK in FB and c-Kit in interstitial Cajal-like cells (ICLC) were completely absent. By transmission electron microscopy in Cav-1(+/+), regular size caveolae (Cav) were at CEC, irregular size Cav were at CM and a few were at FB. In Cav-1(-/-) there were few Cav at CM and FB and some at CEC. To conclude, MMP-2 is closely associated with caveolins at FB and CEC as well as at CM. Also, MMP-2 is closely associated with FAK at FB and c-Kit at ICLC. Thus, Cav-1 expression is not necessary for Cav-2 expression. Cav-3 or Cav-3 with Cav-2 has the capability to make Cav.

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Electron micrographs of CM, FB and CEC in Cav-1+/+ and Cav-1−/−mouse LVM. In Cav-1+/+ mouse A–C shows that CM, FB and CEC are closely located. Some caveolae (Cav, arrows) reveal at CM sar-colemma and a number of (Cav, arrows) reveal at CEC membrane. Well-developed sarcoplasmic retic-ulums (SR, closed thin arrows) are continuously arranged between myofilaments in CM and a few endoplasmic reticulums (ER, open thin arrows) reveal near by Cav at CEC membrane. Sacs of T-tubules (arrowheads) reveal adjacent to Cav and SR of CM and large mito-chondria (m) locate adjacent to sar-colemma. FB does not have Cav but have well-developed rough ER. In Cav-1−/− mouse D–F shows that CM, FB and CEC are closely located as Cav-1+/+ mouse. A few Cav (arrow) reveal at the sarcolemma and some flat-shaped SR (closed thin arrows) along the sarcolemma are discontinuously located in CM. Sacs of T-tubules (arrowheads) reveal adjacent to SR. CEC have a few Cav. FB does not have Cav, but a few have it. FB-p, process of fibroblast.
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fig08: Electron micrographs of CM, FB and CEC in Cav-1+/+ and Cav-1−/−mouse LVM. In Cav-1+/+ mouse A–C shows that CM, FB and CEC are closely located. Some caveolae (Cav, arrows) reveal at CM sar-colemma and a number of (Cav, arrows) reveal at CEC membrane. Well-developed sarcoplasmic retic-ulums (SR, closed thin arrows) are continuously arranged between myofilaments in CM and a few endoplasmic reticulums (ER, open thin arrows) reveal near by Cav at CEC membrane. Sacs of T-tubules (arrowheads) reveal adjacent to Cav and SR of CM and large mito-chondria (m) locate adjacent to sar-colemma. FB does not have Cav but have well-developed rough ER. In Cav-1−/− mouse D–F shows that CM, FB and CEC are closely located as Cav-1+/+ mouse. A few Cav (arrow) reveal at the sarcolemma and some flat-shaped SR (closed thin arrows) along the sarcolemma are discontinuously located in CM. Sacs of T-tubules (arrowheads) reveal adjacent to SR. CEC have a few Cav. FB does not have Cav, but a few have it. FB-p, process of fibroblast.

Mentions: In longitudinal sections of CM of Cav-1+/+ mouse LVM CM revealed some opened Cav, flask-shaped invaginations at the sarcolemma. An abundance of large mitochondria interrupted myofilaments without clearly defined limits. Well-developed sarcoplasmic reticulum (SR) was arranged continuously along sarcolemma and between masses of myofilaments. Spherical lipid droplets were often located between the ends of the mitochondria. Glycogen particles in the form of dense granules were crowded into the interstices among the mitochondria and near by Z-lines (Fig. 8A and B). In longitudinal sections of CM of Cav-1−/− mouse LVM CM revealed an abundance of large mitochondria and mass of myofilaments, as in control cells. However, a few opened Cav were present at the sarcolemma and some SR along sarcolem-ma was discontinuously located (Fig. 8D and E).


Matrix metalloproteinase-2, caveolins, focal adhesion kinase and c-Kit in cells of the mouse myocardium.

Cho WJ, Chow AK, Schulz R, Daniel EE - J. Cell. Mol. Med. (2007 Sep-Oct)

Electron micrographs of CM, FB and CEC in Cav-1+/+ and Cav-1−/−mouse LVM. In Cav-1+/+ mouse A–C shows that CM, FB and CEC are closely located. Some caveolae (Cav, arrows) reveal at CM sar-colemma and a number of (Cav, arrows) reveal at CEC membrane. Well-developed sarcoplasmic retic-ulums (SR, closed thin arrows) are continuously arranged between myofilaments in CM and a few endoplasmic reticulums (ER, open thin arrows) reveal near by Cav at CEC membrane. Sacs of T-tubules (arrowheads) reveal adjacent to Cav and SR of CM and large mito-chondria (m) locate adjacent to sar-colemma. FB does not have Cav but have well-developed rough ER. In Cav-1−/− mouse D–F shows that CM, FB and CEC are closely located as Cav-1+/+ mouse. A few Cav (arrow) reveal at the sarcolemma and some flat-shaped SR (closed thin arrows) along the sarcolemma are discontinuously located in CM. Sacs of T-tubules (arrowheads) reveal adjacent to SR. CEC have a few Cav. FB does not have Cav, but a few have it. FB-p, process of fibroblast.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4401273&req=5

fig08: Electron micrographs of CM, FB and CEC in Cav-1+/+ and Cav-1−/−mouse LVM. In Cav-1+/+ mouse A–C shows that CM, FB and CEC are closely located. Some caveolae (Cav, arrows) reveal at CM sar-colemma and a number of (Cav, arrows) reveal at CEC membrane. Well-developed sarcoplasmic retic-ulums (SR, closed thin arrows) are continuously arranged between myofilaments in CM and a few endoplasmic reticulums (ER, open thin arrows) reveal near by Cav at CEC membrane. Sacs of T-tubules (arrowheads) reveal adjacent to Cav and SR of CM and large mito-chondria (m) locate adjacent to sar-colemma. FB does not have Cav but have well-developed rough ER. In Cav-1−/− mouse D–F shows that CM, FB and CEC are closely located as Cav-1+/+ mouse. A few Cav (arrow) reveal at the sarcolemma and some flat-shaped SR (closed thin arrows) along the sarcolemma are discontinuously located in CM. Sacs of T-tubules (arrowheads) reveal adjacent to SR. CEC have a few Cav. FB does not have Cav, but a few have it. FB-p, process of fibroblast.
Mentions: In longitudinal sections of CM of Cav-1+/+ mouse LVM CM revealed some opened Cav, flask-shaped invaginations at the sarcolemma. An abundance of large mitochondria interrupted myofilaments without clearly defined limits. Well-developed sarcoplasmic reticulum (SR) was arranged continuously along sarcolemma and between masses of myofilaments. Spherical lipid droplets were often located between the ends of the mitochondria. Glycogen particles in the form of dense granules were crowded into the interstices among the mitochondria and near by Z-lines (Fig. 8A and B). In longitudinal sections of CM of Cav-1−/− mouse LVM CM revealed an abundance of large mitochondria and mass of myofilaments, as in control cells. However, a few opened Cav were present at the sarcolemma and some SR along sarcolem-ma was discontinuously located (Fig. 8D and E).

Bottom Line: In Cav-1(-/-) LV Cav-1 and MMP-2 were absent or reduced everywhere.To conclude, MMP-2 is closely associated with caveolins at FB and CEC as well as at CM.Also, MMP-2 is closely associated with FAK at FB and c-Kit at ICLC.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada.

ABSTRACT
Matrix metalloproteinase-2 (MMP-2) may play roles at intracellular and extracellular sites of the heart in ischaemia/reperfusion injury. Caveolins (Cav-1, -2 and -3) are lipid raft proteins which play roles in cell sig-nalling. This study examined, using immunohistochemistry and two photon confocal microscopy, if MMP-2 and caveolins co-localize at the plasma membrane of cardiac cells: cardiomyocytes (CM), fibroblasts (FB) and capillary endothelial cells (CEC) in the left ventricle (LV) of the Cav-1(+/+) and Cav-1(-/-) mouse heart. In Cav-1(+/+) mouse LV MMP-2 and Cav-1 co-localized at CM plasma membranes, and at multiple locations in FB and CEC. MMP-2 co-localized with Cav-2 only at CEC. MMP-2 co-localized with Cav-3 at CM plasma membranes and Z-lines, and partially at FB and CEC. In Cav-1(-/-) LV Cav-1 and MMP-2 were absent or reduced everywhere. Cav-2 appeared at CEC despite the absence of Cav-1. Cav-3 appeared at CM plasma membranes and Z-lines, FB and CEC. Also, FAK in FB and c-Kit in interstitial Cajal-like cells (ICLC) were completely absent. By transmission electron microscopy in Cav-1(+/+), regular size caveolae (Cav) were at CEC, irregular size Cav were at CM and a few were at FB. In Cav-1(-/-) there were few Cav at CM and FB and some at CEC. To conclude, MMP-2 is closely associated with caveolins at FB and CEC as well as at CM. Also, MMP-2 is closely associated with FAK at FB and c-Kit at ICLC. Thus, Cav-1 expression is not necessary for Cav-2 expression. Cav-3 or Cav-3 with Cav-2 has the capability to make Cav.

Show MeSH
Related in: MedlinePlus