Limits...
Matrix metalloproteinase-2, caveolins, focal adhesion kinase and c-Kit in cells of the mouse myocardium.

Cho WJ, Chow AK, Schulz R, Daniel EE - J. Cell. Mol. Med. (2007 Sep-Oct)

Bottom Line: In Cav-1(-/-) LV Cav-1 and MMP-2 were absent or reduced everywhere.To conclude, MMP-2 is closely associated with caveolins at FB and CEC as well as at CM.Also, MMP-2 is closely associated with FAK at FB and c-Kit at ICLC.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada.

ABSTRACT
Matrix metalloproteinase-2 (MMP-2) may play roles at intracellular and extracellular sites of the heart in ischaemia/reperfusion injury. Caveolins (Cav-1, -2 and -3) are lipid raft proteins which play roles in cell sig-nalling. This study examined, using immunohistochemistry and two photon confocal microscopy, if MMP-2 and caveolins co-localize at the plasma membrane of cardiac cells: cardiomyocytes (CM), fibroblasts (FB) and capillary endothelial cells (CEC) in the left ventricle (LV) of the Cav-1(+/+) and Cav-1(-/-) mouse heart. In Cav-1(+/+) mouse LV MMP-2 and Cav-1 co-localized at CM plasma membranes, and at multiple locations in FB and CEC. MMP-2 co-localized with Cav-2 only at CEC. MMP-2 co-localized with Cav-3 at CM plasma membranes and Z-lines, and partially at FB and CEC. In Cav-1(-/-) LV Cav-1 and MMP-2 were absent or reduced everywhere. Cav-2 appeared at CEC despite the absence of Cav-1. Cav-3 appeared at CM plasma membranes and Z-lines, FB and CEC. Also, FAK in FB and c-Kit in interstitial Cajal-like cells (ICLC) were completely absent. By transmission electron microscopy in Cav-1(+/+), regular size caveolae (Cav) were at CEC, irregular size Cav were at CM and a few were at FB. In Cav-1(-/-) there were few Cav at CM and FB and some at CEC. To conclude, MMP-2 is closely associated with caveolins at FB and CEC as well as at CM. Also, MMP-2 is closely associated with FAK at FB and c-Kit at ICLC. Thus, Cav-1 expression is not necessary for Cav-2 expression. Cav-3 or Cav-3 with Cav-2 has the capability to make Cav.

Show MeSH

Related in: MedlinePlus

Localization of FAK and vWF and co-localization of DDR-2 and c-Kit in Cav-1+/+ and Cav-1−/− mouse LVM. In Cav-1+/+ mouse FAK (red, open arrows) appears at FB and vWF (green, closed arrows) does at CEC, but these are not co-localized (A–C). Interestingly some DDR-2-positive FB (green, small open arrow) are c-Kit-positive (red, small closed arrows) and these are partially co-localized (yellow, large open arrows) (E–G). In Cav-1−/− mouse FAK is completely absent but vWF (green, arrows) appears at CEC (D). c-Kit, also, is completely absent but DDR-2 (green, arrows) appears at FB (H). CM is cardiomyocyte and IS is interstitial space. Scale bar is 10 μm for all images.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4401273&req=5

fig07: Localization of FAK and vWF and co-localization of DDR-2 and c-Kit in Cav-1+/+ and Cav-1−/− mouse LVM. In Cav-1+/+ mouse FAK (red, open arrows) appears at FB and vWF (green, closed arrows) does at CEC, but these are not co-localized (A–C). Interestingly some DDR-2-positive FB (green, small open arrow) are c-Kit-positive (red, small closed arrows) and these are partially co-localized (yellow, large open arrows) (E–G). In Cav-1−/− mouse FAK is completely absent but vWF (green, arrows) appears at CEC (D). c-Kit, also, is completely absent but DDR-2 (green, arrows) appears at FB (H). CM is cardiomyocyte and IS is interstitial space. Scale bar is 10 μm for all images.

Mentions: An experiment to determine if FAK and vWF were ever present in the same cell was essential to determine that these markers discriminated between FB and CEC. FAK was found to be present specifically at FB and non-specifically at nuclei of all cell types of Cav-1+/+ mouse LVM (Fig. 7A–C). Moreover, vWF was specifically present at CEC and endothelial cell of both Cav-1+/+ and Cav-1−/− mouse LVM (Fig. 7D).


Matrix metalloproteinase-2, caveolins, focal adhesion kinase and c-Kit in cells of the mouse myocardium.

Cho WJ, Chow AK, Schulz R, Daniel EE - J. Cell. Mol. Med. (2007 Sep-Oct)

Localization of FAK and vWF and co-localization of DDR-2 and c-Kit in Cav-1+/+ and Cav-1−/− mouse LVM. In Cav-1+/+ mouse FAK (red, open arrows) appears at FB and vWF (green, closed arrows) does at CEC, but these are not co-localized (A–C). Interestingly some DDR-2-positive FB (green, small open arrow) are c-Kit-positive (red, small closed arrows) and these are partially co-localized (yellow, large open arrows) (E–G). In Cav-1−/− mouse FAK is completely absent but vWF (green, arrows) appears at CEC (D). c-Kit, also, is completely absent but DDR-2 (green, arrows) appears at FB (H). CM is cardiomyocyte and IS is interstitial space. Scale bar is 10 μm for all images.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4401273&req=5

fig07: Localization of FAK and vWF and co-localization of DDR-2 and c-Kit in Cav-1+/+ and Cav-1−/− mouse LVM. In Cav-1+/+ mouse FAK (red, open arrows) appears at FB and vWF (green, closed arrows) does at CEC, but these are not co-localized (A–C). Interestingly some DDR-2-positive FB (green, small open arrow) are c-Kit-positive (red, small closed arrows) and these are partially co-localized (yellow, large open arrows) (E–G). In Cav-1−/− mouse FAK is completely absent but vWF (green, arrows) appears at CEC (D). c-Kit, also, is completely absent but DDR-2 (green, arrows) appears at FB (H). CM is cardiomyocyte and IS is interstitial space. Scale bar is 10 μm for all images.
Mentions: An experiment to determine if FAK and vWF were ever present in the same cell was essential to determine that these markers discriminated between FB and CEC. FAK was found to be present specifically at FB and non-specifically at nuclei of all cell types of Cav-1+/+ mouse LVM (Fig. 7A–C). Moreover, vWF was specifically present at CEC and endothelial cell of both Cav-1+/+ and Cav-1−/− mouse LVM (Fig. 7D).

Bottom Line: In Cav-1(-/-) LV Cav-1 and MMP-2 were absent or reduced everywhere.To conclude, MMP-2 is closely associated with caveolins at FB and CEC as well as at CM.Also, MMP-2 is closely associated with FAK at FB and c-Kit at ICLC.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada.

ABSTRACT
Matrix metalloproteinase-2 (MMP-2) may play roles at intracellular and extracellular sites of the heart in ischaemia/reperfusion injury. Caveolins (Cav-1, -2 and -3) are lipid raft proteins which play roles in cell sig-nalling. This study examined, using immunohistochemistry and two photon confocal microscopy, if MMP-2 and caveolins co-localize at the plasma membrane of cardiac cells: cardiomyocytes (CM), fibroblasts (FB) and capillary endothelial cells (CEC) in the left ventricle (LV) of the Cav-1(+/+) and Cav-1(-/-) mouse heart. In Cav-1(+/+) mouse LV MMP-2 and Cav-1 co-localized at CM plasma membranes, and at multiple locations in FB and CEC. MMP-2 co-localized with Cav-2 only at CEC. MMP-2 co-localized with Cav-3 at CM plasma membranes and Z-lines, and partially at FB and CEC. In Cav-1(-/-) LV Cav-1 and MMP-2 were absent or reduced everywhere. Cav-2 appeared at CEC despite the absence of Cav-1. Cav-3 appeared at CM plasma membranes and Z-lines, FB and CEC. Also, FAK in FB and c-Kit in interstitial Cajal-like cells (ICLC) were completely absent. By transmission electron microscopy in Cav-1(+/+), regular size caveolae (Cav) were at CEC, irregular size Cav were at CM and a few were at FB. In Cav-1(-/-) there were few Cav at CM and FB and some at CEC. To conclude, MMP-2 is closely associated with caveolins at FB and CEC as well as at CM. Also, MMP-2 is closely associated with FAK at FB and c-Kit at ICLC. Thus, Cav-1 expression is not necessary for Cav-2 expression. Cav-3 or Cav-3 with Cav-2 has the capability to make Cav.

Show MeSH
Related in: MedlinePlus