Matrix metalloproteinase-2, caveolins, focal adhesion kinase and c-Kit in cells of the mouse myocardium.
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In Cav-1(-/-) LV Cav-1 and MMP-2 were absent or reduced everywhere.To conclude, MMP-2 is closely associated with caveolins at FB and CEC as well as at CM.Also, MMP-2 is closely associated with FAK at FB and c-Kit at ICLC.
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PubMed Central - PubMed
Affiliation: Department of Pharmacology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada.
ABSTRACT
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Matrix metalloproteinase-2 (MMP-2) may play roles at intracellular and extracellular sites of the heart in ischaemia/reperfusion injury. Caveolins (Cav-1, -2 and -3) are lipid raft proteins which play roles in cell sig-nalling. This study examined, using immunohistochemistry and two photon confocal microscopy, if MMP-2 and caveolins co-localize at the plasma membrane of cardiac cells: cardiomyocytes (CM), fibroblasts (FB) and capillary endothelial cells (CEC) in the left ventricle (LV) of the Cav-1(+/+) and Cav-1(-/-) mouse heart. In Cav-1(+/+) mouse LV MMP-2 and Cav-1 co-localized at CM plasma membranes, and at multiple locations in FB and CEC. MMP-2 co-localized with Cav-2 only at CEC. MMP-2 co-localized with Cav-3 at CM plasma membranes and Z-lines, and partially at FB and CEC. In Cav-1(-/-) LV Cav-1 and MMP-2 were absent or reduced everywhere. Cav-2 appeared at CEC despite the absence of Cav-1. Cav-3 appeared at CM plasma membranes and Z-lines, FB and CEC. Also, FAK in FB and c-Kit in interstitial Cajal-like cells (ICLC) were completely absent. By transmission electron microscopy in Cav-1(+/+), regular size caveolae (Cav) were at CEC, irregular size Cav were at CM and a few were at FB. In Cav-1(-/-) there were few Cav at CM and FB and some at CEC. To conclude, MMP-2 is closely associated with caveolins at FB and CEC as well as at CM. Also, MMP-2 is closely associated with FAK at FB and c-Kit at ICLC. Thus, Cav-1 expression is not necessary for Cav-2 expression. Cav-3 or Cav-3 with Cav-2 has the capability to make Cav. Related in: MedlinePlus |
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fig05: Co-localization of Cav-2 and FAK / c-Kit in Cav-1+/+ and Cav-1−/− mouse LVM. In Cav-1+/+ Cav-2 (green) (closed arrow) appears only at CEC (nucleus: small asterisks) and FAK (red) (open arrow) only at FB (nucleus: large asterisks). An anastomotic structure appears (open arrowhead) (A–C). Cav-2 (green) doesn't co-localize with c-Kit (red). Arrow indicates for FB, large asterisk for FB nucleus and small asterisk for CEC nucleus (E–G). In Cav-1−/− FAK and c-Kit are missing with Cav-1 (D and H). CM is cardiomyocyte and IS is interstitial space. Scale bar is 10 mm for all images. Mentions: 3D reconstructed images in Figure 5A–C revealed that FAK-FB was positioned close to CEC with Cav-2, but there was no co-localization between two proteins in Cav-1+/+ mouse LVM. Figure 5d revealed the presence only of Cav-2 in the non-nuclear components of cells in Cav-1−/− mouse LVM. |
View Article: PubMed Central - PubMed
Affiliation: Department of Pharmacology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada.