Limits...
Matrix metalloproteinase-2, caveolins, focal adhesion kinase and c-Kit in cells of the mouse myocardium.

Cho WJ, Chow AK, Schulz R, Daniel EE - J. Cell. Mol. Med. (2007 Sep-Oct)

Bottom Line: In Cav-1(-/-) LV Cav-1 and MMP-2 were absent or reduced everywhere.To conclude, MMP-2 is closely associated with caveolins at FB and CEC as well as at CM.Also, MMP-2 is closely associated with FAK at FB and c-Kit at ICLC.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada.

ABSTRACT
Matrix metalloproteinase-2 (MMP-2) may play roles at intracellular and extracellular sites of the heart in ischaemia/reperfusion injury. Caveolins (Cav-1, -2 and -3) are lipid raft proteins which play roles in cell sig-nalling. This study examined, using immunohistochemistry and two photon confocal microscopy, if MMP-2 and caveolins co-localize at the plasma membrane of cardiac cells: cardiomyocytes (CM), fibroblasts (FB) and capillary endothelial cells (CEC) in the left ventricle (LV) of the Cav-1(+/+) and Cav-1(-/-) mouse heart. In Cav-1(+/+) mouse LV MMP-2 and Cav-1 co-localized at CM plasma membranes, and at multiple locations in FB and CEC. MMP-2 co-localized with Cav-2 only at CEC. MMP-2 co-localized with Cav-3 at CM plasma membranes and Z-lines, and partially at FB and CEC. In Cav-1(-/-) LV Cav-1 and MMP-2 were absent or reduced everywhere. Cav-2 appeared at CEC despite the absence of Cav-1. Cav-3 appeared at CM plasma membranes and Z-lines, FB and CEC. Also, FAK in FB and c-Kit in interstitial Cajal-like cells (ICLC) were completely absent. By transmission electron microscopy in Cav-1(+/+), regular size caveolae (Cav) were at CEC, irregular size Cav were at CM and a few were at FB. In Cav-1(-/-) there were few Cav at CM and FB and some at CEC. To conclude, MMP-2 is closely associated with caveolins at FB and CEC as well as at CM. Also, MMP-2 is closely associated with FAK at FB and c-Kit at ICLC. Thus, Cav-1 expression is not necessary for Cav-2 expression. Cav-3 or Cav-3 with Cav-2 has the capability to make Cav.

Show MeSH

Related in: MedlinePlus

Co-localization of MMP-2 and FAK / DDR-2 / vWF / c-Kit in Cav-1+/+ and Cav-1−/− mouse LVM. In Cav-1+/+ co-localization (arrows) of MMP-2 (green) and FAK (red) (A–C), MMP-2 (green) and DDR-2 (red) (E–G), and MMP-2 (red) and vWF (green) (I–K) shows that MMP-2 localizes at FB (nucleus:large asterisks) as well as at CEC (nucleus:small asterisks). MMP-2 (green) also co-localizes (arrows) with c-Kit (red) at ICLC near by CM (M-N). In Cav-1−/−, surprisingly, FAK (red) and c-Kit (red) are completely absent at FB and ICLC, respectively, except FAK in nuclei of all cells (Dand P).vWF, however, is still present at CEC (I). CM is car-diomyocyte and IS is interstitial space. Scale bar is 10 mm for all images.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4401273&req=5

fig02: Co-localization of MMP-2 and FAK / DDR-2 / vWF / c-Kit in Cav-1+/+ and Cav-1−/− mouse LVM. In Cav-1+/+ co-localization (arrows) of MMP-2 (green) and FAK (red) (A–C), MMP-2 (green) and DDR-2 (red) (E–G), and MMP-2 (red) and vWF (green) (I–K) shows that MMP-2 localizes at FB (nucleus:large asterisks) as well as at CEC (nucleus:small asterisks). MMP-2 (green) also co-localizes (arrows) with c-Kit (red) at ICLC near by CM (M-N). In Cav-1−/−, surprisingly, FAK (red) and c-Kit (red) are completely absent at FB and ICLC, respectively, except FAK in nuclei of all cells (Dand P).vWF, however, is still present at CEC (I). CM is car-diomyocyte and IS is interstitial space. Scale bar is 10 mm for all images.

Mentions: FAK immunoreactivity appeared at FB which ran alongside CM and CEC. In Figure 2A–C, one FAK-positive FB (FAK-FB) was connected to another FAK-FB by an elongated thin tail. MMP-2 appeared in the FAK-positive FB and co-localized with FAK in Cav-1+/+ mouse LVM (Fig. 2A–C). On the other hand, in Cav-1−/− mouse LVM, FAK was absent at FB (Fig. 2D).


Matrix metalloproteinase-2, caveolins, focal adhesion kinase and c-Kit in cells of the mouse myocardium.

Cho WJ, Chow AK, Schulz R, Daniel EE - J. Cell. Mol. Med. (2007 Sep-Oct)

Co-localization of MMP-2 and FAK / DDR-2 / vWF / c-Kit in Cav-1+/+ and Cav-1−/− mouse LVM. In Cav-1+/+ co-localization (arrows) of MMP-2 (green) and FAK (red) (A–C), MMP-2 (green) and DDR-2 (red) (E–G), and MMP-2 (red) and vWF (green) (I–K) shows that MMP-2 localizes at FB (nucleus:large asterisks) as well as at CEC (nucleus:small asterisks). MMP-2 (green) also co-localizes (arrows) with c-Kit (red) at ICLC near by CM (M-N). In Cav-1−/−, surprisingly, FAK (red) and c-Kit (red) are completely absent at FB and ICLC, respectively, except FAK in nuclei of all cells (Dand P).vWF, however, is still present at CEC (I). CM is car-diomyocyte and IS is interstitial space. Scale bar is 10 mm for all images.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4401273&req=5

fig02: Co-localization of MMP-2 and FAK / DDR-2 / vWF / c-Kit in Cav-1+/+ and Cav-1−/− mouse LVM. In Cav-1+/+ co-localization (arrows) of MMP-2 (green) and FAK (red) (A–C), MMP-2 (green) and DDR-2 (red) (E–G), and MMP-2 (red) and vWF (green) (I–K) shows that MMP-2 localizes at FB (nucleus:large asterisks) as well as at CEC (nucleus:small asterisks). MMP-2 (green) also co-localizes (arrows) with c-Kit (red) at ICLC near by CM (M-N). In Cav-1−/−, surprisingly, FAK (red) and c-Kit (red) are completely absent at FB and ICLC, respectively, except FAK in nuclei of all cells (Dand P).vWF, however, is still present at CEC (I). CM is car-diomyocyte and IS is interstitial space. Scale bar is 10 mm for all images.
Mentions: FAK immunoreactivity appeared at FB which ran alongside CM and CEC. In Figure 2A–C, one FAK-positive FB (FAK-FB) was connected to another FAK-FB by an elongated thin tail. MMP-2 appeared in the FAK-positive FB and co-localized with FAK in Cav-1+/+ mouse LVM (Fig. 2A–C). On the other hand, in Cav-1−/− mouse LVM, FAK was absent at FB (Fig. 2D).

Bottom Line: In Cav-1(-/-) LV Cav-1 and MMP-2 were absent or reduced everywhere.To conclude, MMP-2 is closely associated with caveolins at FB and CEC as well as at CM.Also, MMP-2 is closely associated with FAK at FB and c-Kit at ICLC.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada.

ABSTRACT
Matrix metalloproteinase-2 (MMP-2) may play roles at intracellular and extracellular sites of the heart in ischaemia/reperfusion injury. Caveolins (Cav-1, -2 and -3) are lipid raft proteins which play roles in cell sig-nalling. This study examined, using immunohistochemistry and two photon confocal microscopy, if MMP-2 and caveolins co-localize at the plasma membrane of cardiac cells: cardiomyocytes (CM), fibroblasts (FB) and capillary endothelial cells (CEC) in the left ventricle (LV) of the Cav-1(+/+) and Cav-1(-/-) mouse heart. In Cav-1(+/+) mouse LV MMP-2 and Cav-1 co-localized at CM plasma membranes, and at multiple locations in FB and CEC. MMP-2 co-localized with Cav-2 only at CEC. MMP-2 co-localized with Cav-3 at CM plasma membranes and Z-lines, and partially at FB and CEC. In Cav-1(-/-) LV Cav-1 and MMP-2 were absent or reduced everywhere. Cav-2 appeared at CEC despite the absence of Cav-1. Cav-3 appeared at CM plasma membranes and Z-lines, FB and CEC. Also, FAK in FB and c-Kit in interstitial Cajal-like cells (ICLC) were completely absent. By transmission electron microscopy in Cav-1(+/+), regular size caveolae (Cav) were at CEC, irregular size Cav were at CM and a few were at FB. In Cav-1(-/-) there were few Cav at CM and FB and some at CEC. To conclude, MMP-2 is closely associated with caveolins at FB and CEC as well as at CM. Also, MMP-2 is closely associated with FAK at FB and c-Kit at ICLC. Thus, Cav-1 expression is not necessary for Cav-2 expression. Cav-3 or Cav-3 with Cav-2 has the capability to make Cav.

Show MeSH
Related in: MedlinePlus