Limits...
Matrix metalloproteinase-2, caveolins, focal adhesion kinase and c-Kit in cells of the mouse myocardium.

Cho WJ, Chow AK, Schulz R, Daniel EE - J. Cell. Mol. Med. (2007 Sep-Oct)

Bottom Line: In Cav-1(-/-) LV Cav-1 and MMP-2 were absent or reduced everywhere.To conclude, MMP-2 is closely associated with caveolins at FB and CEC as well as at CM.Also, MMP-2 is closely associated with FAK at FB and c-Kit at ICLC.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada.

ABSTRACT
Matrix metalloproteinase-2 (MMP-2) may play roles at intracellular and extracellular sites of the heart in ischaemia/reperfusion injury. Caveolins (Cav-1, -2 and -3) are lipid raft proteins which play roles in cell sig-nalling. This study examined, using immunohistochemistry and two photon confocal microscopy, if MMP-2 and caveolins co-localize at the plasma membrane of cardiac cells: cardiomyocytes (CM), fibroblasts (FB) and capillary endothelial cells (CEC) in the left ventricle (LV) of the Cav-1(+/+) and Cav-1(-/-) mouse heart. In Cav-1(+/+) mouse LV MMP-2 and Cav-1 co-localized at CM plasma membranes, and at multiple locations in FB and CEC. MMP-2 co-localized with Cav-2 only at CEC. MMP-2 co-localized with Cav-3 at CM plasma membranes and Z-lines, and partially at FB and CEC. In Cav-1(-/-) LV Cav-1 and MMP-2 were absent or reduced everywhere. Cav-2 appeared at CEC despite the absence of Cav-1. Cav-3 appeared at CM plasma membranes and Z-lines, FB and CEC. Also, FAK in FB and c-Kit in interstitial Cajal-like cells (ICLC) were completely absent. By transmission electron microscopy in Cav-1(+/+), regular size caveolae (Cav) were at CEC, irregular size Cav were at CM and a few were at FB. In Cav-1(-/-) there were few Cav at CM and FB and some at CEC. To conclude, MMP-2 is closely associated with caveolins at FB and CEC as well as at CM. Also, MMP-2 is closely associated with FAK at FB and c-Kit at ICLC. Thus, Cav-1 expression is not necessary for Cav-2 expression. Cav-3 or Cav-3 with Cav-2 has the capability to make Cav.

Show MeSH

Related in: MedlinePlus

Co-localization of MMP-2 and Caveolins in Cav-1+/+ and Cav-1−/− mouse LVM. In Cav-1+/+ MMP-2 (red) appeared to have good co-localization with Cav-1 (green) at CM plasma membranes (large open arrows), CEC (closed arrows) and FB (small open arrows), and partial co-localization occurs at Z-lines (open arrowheads) (A–C). MMP-2 (red), however, appeared to have good co-localization with Cav-2 (green) only at CEC (closed arrows). FB with MMP-2 appear (open arrows) (E–G). MMP-2 (red) appeared to have good co-localization with Cav-3 (green) at CM plasma membranes (open arrows) and Z-lines (arrowhead), and partial co-localization at FB (closed arrows) (I–K). In Cav-1−/− Cav-1 is absent and MMP-2 is markedly reduced at CM plasma membrane (arrow), CEC and FB (D). IS refers to interstitial space. Interestingly, Cav-2 (green) appears regardless of Cav-1 only at CEC (H) and Cav-3 (green) appears at CM plasma membranes (large arrows), Z-lines (arrowhead), and FB (small arrows) like in Cav-1+/+(I). CM is cardiomyocyte and IS is interstitial space. Scale bar is 10 mm for A–H and I, and 20 mm for I–K.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4401273&req=5

fig01: Co-localization of MMP-2 and Caveolins in Cav-1+/+ and Cav-1−/− mouse LVM. In Cav-1+/+ MMP-2 (red) appeared to have good co-localization with Cav-1 (green) at CM plasma membranes (large open arrows), CEC (closed arrows) and FB (small open arrows), and partial co-localization occurs at Z-lines (open arrowheads) (A–C). MMP-2 (red), however, appeared to have good co-localization with Cav-2 (green) only at CEC (closed arrows). FB with MMP-2 appear (open arrows) (E–G). MMP-2 (red) appeared to have good co-localization with Cav-3 (green) at CM plasma membranes (open arrows) and Z-lines (arrowhead), and partial co-localization at FB (closed arrows) (I–K). In Cav-1−/− Cav-1 is absent and MMP-2 is markedly reduced at CM plasma membrane (arrow), CEC and FB (D). IS refers to interstitial space. Interestingly, Cav-2 (green) appears regardless of Cav-1 only at CEC (H) and Cav-3 (green) appears at CM plasma membranes (large arrows), Z-lines (arrowhead), and FB (small arrows) like in Cav-1+/+(I). CM is cardiomyocyte and IS is interstitial space. Scale bar is 10 mm for A–H and I, and 20 mm for I–K.

Mentions: MMP-2 co-localized well with Cav-1 at the CM plasma membrane, FB and CEC, and partially at the Z-lines in Cav-1+/+ mouse LVM (Fig. 1A–C). In Cav-1−/− mouse LVM, MMP-2 was significantly reduced or absent in all these cells (Fig. 1D).


Matrix metalloproteinase-2, caveolins, focal adhesion kinase and c-Kit in cells of the mouse myocardium.

Cho WJ, Chow AK, Schulz R, Daniel EE - J. Cell. Mol. Med. (2007 Sep-Oct)

Co-localization of MMP-2 and Caveolins in Cav-1+/+ and Cav-1−/− mouse LVM. In Cav-1+/+ MMP-2 (red) appeared to have good co-localization with Cav-1 (green) at CM plasma membranes (large open arrows), CEC (closed arrows) and FB (small open arrows), and partial co-localization occurs at Z-lines (open arrowheads) (A–C). MMP-2 (red), however, appeared to have good co-localization with Cav-2 (green) only at CEC (closed arrows). FB with MMP-2 appear (open arrows) (E–G). MMP-2 (red) appeared to have good co-localization with Cav-3 (green) at CM plasma membranes (open arrows) and Z-lines (arrowhead), and partial co-localization at FB (closed arrows) (I–K). In Cav-1−/− Cav-1 is absent and MMP-2 is markedly reduced at CM plasma membrane (arrow), CEC and FB (D). IS refers to interstitial space. Interestingly, Cav-2 (green) appears regardless of Cav-1 only at CEC (H) and Cav-3 (green) appears at CM plasma membranes (large arrows), Z-lines (arrowhead), and FB (small arrows) like in Cav-1+/+(I). CM is cardiomyocyte and IS is interstitial space. Scale bar is 10 mm for A–H and I, and 20 mm for I–K.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4401273&req=5

fig01: Co-localization of MMP-2 and Caveolins in Cav-1+/+ and Cav-1−/− mouse LVM. In Cav-1+/+ MMP-2 (red) appeared to have good co-localization with Cav-1 (green) at CM plasma membranes (large open arrows), CEC (closed arrows) and FB (small open arrows), and partial co-localization occurs at Z-lines (open arrowheads) (A–C). MMP-2 (red), however, appeared to have good co-localization with Cav-2 (green) only at CEC (closed arrows). FB with MMP-2 appear (open arrows) (E–G). MMP-2 (red) appeared to have good co-localization with Cav-3 (green) at CM plasma membranes (open arrows) and Z-lines (arrowhead), and partial co-localization at FB (closed arrows) (I–K). In Cav-1−/− Cav-1 is absent and MMP-2 is markedly reduced at CM plasma membrane (arrow), CEC and FB (D). IS refers to interstitial space. Interestingly, Cav-2 (green) appears regardless of Cav-1 only at CEC (H) and Cav-3 (green) appears at CM plasma membranes (large arrows), Z-lines (arrowhead), and FB (small arrows) like in Cav-1+/+(I). CM is cardiomyocyte and IS is interstitial space. Scale bar is 10 mm for A–H and I, and 20 mm for I–K.
Mentions: MMP-2 co-localized well with Cav-1 at the CM plasma membrane, FB and CEC, and partially at the Z-lines in Cav-1+/+ mouse LVM (Fig. 1A–C). In Cav-1−/− mouse LVM, MMP-2 was significantly reduced or absent in all these cells (Fig. 1D).

Bottom Line: In Cav-1(-/-) LV Cav-1 and MMP-2 were absent or reduced everywhere.To conclude, MMP-2 is closely associated with caveolins at FB and CEC as well as at CM.Also, MMP-2 is closely associated with FAK at FB and c-Kit at ICLC.

View Article: PubMed Central - PubMed

Affiliation: Department of Pharmacology, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada.

ABSTRACT
Matrix metalloproteinase-2 (MMP-2) may play roles at intracellular and extracellular sites of the heart in ischaemia/reperfusion injury. Caveolins (Cav-1, -2 and -3) are lipid raft proteins which play roles in cell sig-nalling. This study examined, using immunohistochemistry and two photon confocal microscopy, if MMP-2 and caveolins co-localize at the plasma membrane of cardiac cells: cardiomyocytes (CM), fibroblasts (FB) and capillary endothelial cells (CEC) in the left ventricle (LV) of the Cav-1(+/+) and Cav-1(-/-) mouse heart. In Cav-1(+/+) mouse LV MMP-2 and Cav-1 co-localized at CM plasma membranes, and at multiple locations in FB and CEC. MMP-2 co-localized with Cav-2 only at CEC. MMP-2 co-localized with Cav-3 at CM plasma membranes and Z-lines, and partially at FB and CEC. In Cav-1(-/-) LV Cav-1 and MMP-2 were absent or reduced everywhere. Cav-2 appeared at CEC despite the absence of Cav-1. Cav-3 appeared at CM plasma membranes and Z-lines, FB and CEC. Also, FAK in FB and c-Kit in interstitial Cajal-like cells (ICLC) were completely absent. By transmission electron microscopy in Cav-1(+/+), regular size caveolae (Cav) were at CEC, irregular size Cav were at CM and a few were at FB. In Cav-1(-/-) there were few Cav at CM and FB and some at CEC. To conclude, MMP-2 is closely associated with caveolins at FB and CEC as well as at CM. Also, MMP-2 is closely associated with FAK at FB and c-Kit at ICLC. Thus, Cav-1 expression is not necessary for Cav-2 expression. Cav-3 or Cav-3 with Cav-2 has the capability to make Cav.

Show MeSH
Related in: MedlinePlus