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Prognostic value of serum angiogenic activity in colorectal cancer patients.

Gonzalez FJ, Quesada AR, Sevilla I, Baca JJ, Medina MA, Amores J, Diaz JM, Rius-Diaz F, Marques E, Alba E - J. Cell. Mol. Med. (2007 Jan-Feb)

Bottom Line: Data were compared with clinical outcome of the patients.Although serum from CRC patients significantly increased the PA of HUVEC, compared to culture control (HUVEC in medium + 10% foetal bovine serum (FBS); P < 0.001); our results indicate that serum PA in CRC patients was similar to that of SC or healthy individuals.Patients with lower PA values after surgery showed a worse outcome that those with higher PA values.

View Article: PubMed Central - PubMed

Affiliation: Medical Oncology Service, Hospital Universitario Virgen de la Victoria, Malaga, Spain. fjgonzalez02@yahoo.es

ABSTRACT
Angiogenesis, resulting from an imbalance between angiogenic activator factors and inhibitors, is required for tumour growth and metastasis. The determination of the circulating concentration of all angiogenic factors (activators and inhibitors) is not feasible at present. We have evaluated diagnostic and prognostic values of the measurement of serum angiogenic activity in colorectal carcinoma (CRC) patients. Serum proliferative activity (PA) on human umbilical vein endothelial cells (HUVEC) in vitro, and serum vascular endothelial growth factor (VEGF) levels were determined by ELISA in 53 patients with primary CRC, 16 subjects with non-neoplastic gastrointestinal disease (SC) and 34 healthy individuals. Data were compared with clinical outcome of the patients. Although serum from CRC patients significantly increased the PA of HUVEC, compared to culture control (HUVEC in medium + 10% foetal bovine serum (FBS); P < 0.001); our results indicate that serum PA in CRC patients was similar to that of SC or healthy individuals. There was no correlation between serum PA and circulating VEGF concentrations. Surgery produced a decrease of PA at 8 hrs after tumour resection in CRC patients compared to pre-surgery values (186 +/- 47 versus 213 +/- 41, P < 0.001). However, an increase in serum VEGF values was observed after surgery (280 [176-450] versus 251 [160-357] pg/ml, P = 0.004). Patients with lower PA values after surgery showed a worse outcome that those with higher PA values. Therefore, this study does not support a diagnostic value for serum angiogenic activity measured by proliferative activity on HUVEC but suggests it could have a prognostic value in CRC patients.

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Kaplan-Meier overall survival curves of 45 patients, following PA values at 8 hrs after surgery less than or equal to cut-off level (n = 23) and greater than cut-off level (n = 22). Patients with PA less than or equal to cut-off level had poor prognostic.
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fig03: Kaplan-Meier overall survival curves of 45 patients, following PA values at 8 hrs after surgery less than or equal to cut-off level (n = 23) and greater than cut-off level (n = 22). Patients with PA less than or equal to cut-off level had poor prognostic.

Mentions: We used a univariate survival analysis considering the patients divided into two groups: PA values less than or equal to median value of PA at 8 hrs after surgery (191, cut-off value), and greater than cut-off value in order to study the differences in overall survival between these two groups. In these groups of patients, considering all patients and only uncensored data, we had a global set of patients and the ranges were (23.14) and (36.77) for uncensored data. After a median global follow-up of 42 months (4–68 months) log-rank test showed a significant difference (P = 0.040) in overall survival rates between both groups (Fig. 3). The percentage of events and values of survival function are shown in Table 3.


Prognostic value of serum angiogenic activity in colorectal cancer patients.

Gonzalez FJ, Quesada AR, Sevilla I, Baca JJ, Medina MA, Amores J, Diaz JM, Rius-Diaz F, Marques E, Alba E - J. Cell. Mol. Med. (2007 Jan-Feb)

Kaplan-Meier overall survival curves of 45 patients, following PA values at 8 hrs after surgery less than or equal to cut-off level (n = 23) and greater than cut-off level (n = 22). Patients with PA less than or equal to cut-off level had poor prognostic.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4401225&req=5

fig03: Kaplan-Meier overall survival curves of 45 patients, following PA values at 8 hrs after surgery less than or equal to cut-off level (n = 23) and greater than cut-off level (n = 22). Patients with PA less than or equal to cut-off level had poor prognostic.
Mentions: We used a univariate survival analysis considering the patients divided into two groups: PA values less than or equal to median value of PA at 8 hrs after surgery (191, cut-off value), and greater than cut-off value in order to study the differences in overall survival between these two groups. In these groups of patients, considering all patients and only uncensored data, we had a global set of patients and the ranges were (23.14) and (36.77) for uncensored data. After a median global follow-up of 42 months (4–68 months) log-rank test showed a significant difference (P = 0.040) in overall survival rates between both groups (Fig. 3). The percentage of events and values of survival function are shown in Table 3.

Bottom Line: Data were compared with clinical outcome of the patients.Although serum from CRC patients significantly increased the PA of HUVEC, compared to culture control (HUVEC in medium + 10% foetal bovine serum (FBS); P < 0.001); our results indicate that serum PA in CRC patients was similar to that of SC or healthy individuals.Patients with lower PA values after surgery showed a worse outcome that those with higher PA values.

View Article: PubMed Central - PubMed

Affiliation: Medical Oncology Service, Hospital Universitario Virgen de la Victoria, Malaga, Spain. fjgonzalez02@yahoo.es

ABSTRACT
Angiogenesis, resulting from an imbalance between angiogenic activator factors and inhibitors, is required for tumour growth and metastasis. The determination of the circulating concentration of all angiogenic factors (activators and inhibitors) is not feasible at present. We have evaluated diagnostic and prognostic values of the measurement of serum angiogenic activity in colorectal carcinoma (CRC) patients. Serum proliferative activity (PA) on human umbilical vein endothelial cells (HUVEC) in vitro, and serum vascular endothelial growth factor (VEGF) levels were determined by ELISA in 53 patients with primary CRC, 16 subjects with non-neoplastic gastrointestinal disease (SC) and 34 healthy individuals. Data were compared with clinical outcome of the patients. Although serum from CRC patients significantly increased the PA of HUVEC, compared to culture control (HUVEC in medium + 10% foetal bovine serum (FBS); P < 0.001); our results indicate that serum PA in CRC patients was similar to that of SC or healthy individuals. There was no correlation between serum PA and circulating VEGF concentrations. Surgery produced a decrease of PA at 8 hrs after tumour resection in CRC patients compared to pre-surgery values (186 +/- 47 versus 213 +/- 41, P < 0.001). However, an increase in serum VEGF values was observed after surgery (280 [176-450] versus 251 [160-357] pg/ml, P = 0.004). Patients with lower PA values after surgery showed a worse outcome that those with higher PA values. Therefore, this study does not support a diagnostic value for serum angiogenic activity measured by proliferative activity on HUVEC but suggests it could have a prognostic value in CRC patients.

Show MeSH
Related in: MedlinePlus