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Prognostic value of serum angiogenic activity in colorectal cancer patients.

Gonzalez FJ, Quesada AR, Sevilla I, Baca JJ, Medina MA, Amores J, Diaz JM, Rius-Diaz F, Marques E, Alba E - J. Cell. Mol. Med. (2007 Jan-Feb)

Bottom Line: Data were compared with clinical outcome of the patients.Although serum from CRC patients significantly increased the PA of HUVEC, compared to culture control (HUVEC in medium + 10% foetal bovine serum (FBS); P < 0.001); our results indicate that serum PA in CRC patients was similar to that of SC or healthy individuals.Patients with lower PA values after surgery showed a worse outcome that those with higher PA values.

View Article: PubMed Central - PubMed

Affiliation: Medical Oncology Service, Hospital Universitario Virgen de la Victoria, Malaga, Spain. fjgonzalez02@yahoo.es

ABSTRACT
Angiogenesis, resulting from an imbalance between angiogenic activator factors and inhibitors, is required for tumour growth and metastasis. The determination of the circulating concentration of all angiogenic factors (activators and inhibitors) is not feasible at present. We have evaluated diagnostic and prognostic values of the measurement of serum angiogenic activity in colorectal carcinoma (CRC) patients. Serum proliferative activity (PA) on human umbilical vein endothelial cells (HUVEC) in vitro, and serum vascular endothelial growth factor (VEGF) levels were determined by ELISA in 53 patients with primary CRC, 16 subjects with non-neoplastic gastrointestinal disease (SC) and 34 healthy individuals. Data were compared with clinical outcome of the patients. Although serum from CRC patients significantly increased the PA of HUVEC, compared to culture control (HUVEC in medium + 10% foetal bovine serum (FBS); P < 0.001); our results indicate that serum PA in CRC patients was similar to that of SC or healthy individuals. There was no correlation between serum PA and circulating VEGF concentrations. Surgery produced a decrease of PA at 8 hrs after tumour resection in CRC patients compared to pre-surgery values (186 +/- 47 versus 213 +/- 41, P < 0.001). However, an increase in serum VEGF values was observed after surgery (280 [176-450] versus 251 [160-357] pg/ml, P = 0.004). Patients with lower PA values after surgery showed a worse outcome that those with higher PA values. Therefore, this study does not support a diagnostic value for serum angiogenic activity measured by proliferative activity on HUVEC but suggests it could have a prognostic value in CRC patients.

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Serum proliferative activity in HUVE cells at 8 hr after surgery tumour removal was lower in CRC patients who died (Dead) during the time of the study compared with patients who were living (Alive) at last follow-up evaluation.
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fig02: Serum proliferative activity in HUVE cells at 8 hr after surgery tumour removal was lower in CRC patients who died (Dead) during the time of the study compared with patients who were living (Alive) at last follow-up evaluation.

Mentions: To study the association between PA values and clinical outcome, 4 patients who died within 30 days after surgery were excluded. Thirty-four of the 49 patients included in the statistical analysis of prognostic values were alive at the time of the last follow-up evaluation, including 1 patient with local recurrence and 2 with distant metastases. Fifteen patients had died of carcinomas. No significant differences were observed in pre-surgery (PA) values between patients who had survived and those who died (n = 34, 213 ± 42 versus n = 15, 208 ± 45). Serum PA was only determined at 8 hrs after surgery in 45 of the 49 patients. Samples taken 8 hrs after surgery from patients who had survived had PA values significantly higher than those from patients who died (n = 31, 196 ± 37 versus n = 14, 157 ± 57; P = 0.009; Fig. 2).


Prognostic value of serum angiogenic activity in colorectal cancer patients.

Gonzalez FJ, Quesada AR, Sevilla I, Baca JJ, Medina MA, Amores J, Diaz JM, Rius-Diaz F, Marques E, Alba E - J. Cell. Mol. Med. (2007 Jan-Feb)

Serum proliferative activity in HUVE cells at 8 hr after surgery tumour removal was lower in CRC patients who died (Dead) during the time of the study compared with patients who were living (Alive) at last follow-up evaluation.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4401225&req=5

fig02: Serum proliferative activity in HUVE cells at 8 hr after surgery tumour removal was lower in CRC patients who died (Dead) during the time of the study compared with patients who were living (Alive) at last follow-up evaluation.
Mentions: To study the association between PA values and clinical outcome, 4 patients who died within 30 days after surgery were excluded. Thirty-four of the 49 patients included in the statistical analysis of prognostic values were alive at the time of the last follow-up evaluation, including 1 patient with local recurrence and 2 with distant metastases. Fifteen patients had died of carcinomas. No significant differences were observed in pre-surgery (PA) values between patients who had survived and those who died (n = 34, 213 ± 42 versus n = 15, 208 ± 45). Serum PA was only determined at 8 hrs after surgery in 45 of the 49 patients. Samples taken 8 hrs after surgery from patients who had survived had PA values significantly higher than those from patients who died (n = 31, 196 ± 37 versus n = 14, 157 ± 57; P = 0.009; Fig. 2).

Bottom Line: Data were compared with clinical outcome of the patients.Although serum from CRC patients significantly increased the PA of HUVEC, compared to culture control (HUVEC in medium + 10% foetal bovine serum (FBS); P < 0.001); our results indicate that serum PA in CRC patients was similar to that of SC or healthy individuals.Patients with lower PA values after surgery showed a worse outcome that those with higher PA values.

View Article: PubMed Central - PubMed

Affiliation: Medical Oncology Service, Hospital Universitario Virgen de la Victoria, Malaga, Spain. fjgonzalez02@yahoo.es

ABSTRACT
Angiogenesis, resulting from an imbalance between angiogenic activator factors and inhibitors, is required for tumour growth and metastasis. The determination of the circulating concentration of all angiogenic factors (activators and inhibitors) is not feasible at present. We have evaluated diagnostic and prognostic values of the measurement of serum angiogenic activity in colorectal carcinoma (CRC) patients. Serum proliferative activity (PA) on human umbilical vein endothelial cells (HUVEC) in vitro, and serum vascular endothelial growth factor (VEGF) levels were determined by ELISA in 53 patients with primary CRC, 16 subjects with non-neoplastic gastrointestinal disease (SC) and 34 healthy individuals. Data were compared with clinical outcome of the patients. Although serum from CRC patients significantly increased the PA of HUVEC, compared to culture control (HUVEC in medium + 10% foetal bovine serum (FBS); P < 0.001); our results indicate that serum PA in CRC patients was similar to that of SC or healthy individuals. There was no correlation between serum PA and circulating VEGF concentrations. Surgery produced a decrease of PA at 8 hrs after tumour resection in CRC patients compared to pre-surgery values (186 +/- 47 versus 213 +/- 41, P < 0.001). However, an increase in serum VEGF values was observed after surgery (280 [176-450] versus 251 [160-357] pg/ml, P = 0.004). Patients with lower PA values after surgery showed a worse outcome that those with higher PA values. Therefore, this study does not support a diagnostic value for serum angiogenic activity measured by proliferative activity on HUVEC but suggests it could have a prognostic value in CRC patients.

Show MeSH
Related in: MedlinePlus