Limits...
Prognostic value of serum angiogenic activity in colorectal cancer patients.

Gonzalez FJ, Quesada AR, Sevilla I, Baca JJ, Medina MA, Amores J, Diaz JM, Rius-Diaz F, Marques E, Alba E - J. Cell. Mol. Med. (2007 Jan-Feb)

Bottom Line: Data were compared with clinical outcome of the patients.Although serum from CRC patients significantly increased the PA of HUVEC, compared to culture control (HUVEC in medium + 10% foetal bovine serum (FBS); P < 0.001); our results indicate that serum PA in CRC patients was similar to that of SC or healthy individuals.Patients with lower PA values after surgery showed a worse outcome that those with higher PA values.

View Article: PubMed Central - PubMed

Affiliation: Medical Oncology Service, Hospital Universitario Virgen de la Victoria, Malaga, Spain. fjgonzalez02@yahoo.es

ABSTRACT
Angiogenesis, resulting from an imbalance between angiogenic activator factors and inhibitors, is required for tumour growth and metastasis. The determination of the circulating concentration of all angiogenic factors (activators and inhibitors) is not feasible at present. We have evaluated diagnostic and prognostic values of the measurement of serum angiogenic activity in colorectal carcinoma (CRC) patients. Serum proliferative activity (PA) on human umbilical vein endothelial cells (HUVEC) in vitro, and serum vascular endothelial growth factor (VEGF) levels were determined by ELISA in 53 patients with primary CRC, 16 subjects with non-neoplastic gastrointestinal disease (SC) and 34 healthy individuals. Data were compared with clinical outcome of the patients. Although serum from CRC patients significantly increased the PA of HUVEC, compared to culture control (HUVEC in medium + 10% foetal bovine serum (FBS); P < 0.001); our results indicate that serum PA in CRC patients was similar to that of SC or healthy individuals. There was no correlation between serum PA and circulating VEGF concentrations. Surgery produced a decrease of PA at 8 hrs after tumour resection in CRC patients compared to pre-surgery values (186 +/- 47 versus 213 +/- 41, P < 0.001). However, an increase in serum VEGF values was observed after surgery (280 [176-450] versus 251 [160-357] pg/ml, P = 0.004). Patients with lower PA values after surgery showed a worse outcome that those with higher PA values. Therefore, this study does not support a diagnostic value for serum angiogenic activity measured by proliferative activity on HUVEC but suggests it could have a prognostic value in CRC patients.

Show MeSH

Related in: MedlinePlus

HUVE cell proliferative activity (PA) induced by serum from colorectal cancer patients (CRC, n = 53), gastrointestinal non-malignant diseases patient (SC, n = 16) and healthy individuals (healthy, n = 34). Values represent relative percentages of proliferation compared to control serum (100%). PA in CRC and SC patients and in healthy individuals were increased compared to control values (P < 0.001).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4401225&req=5

fig01: HUVE cell proliferative activity (PA) induced by serum from colorectal cancer patients (CRC, n = 53), gastrointestinal non-malignant diseases patient (SC, n = 16) and healthy individuals (healthy, n = 34). Values represent relative percentages of proliferation compared to control serum (100%). PA in CRC and SC patients and in healthy individuals were increased compared to control values (P < 0.001).

Mentions: Although the addition of CRC, SC or healthy individuals serum to HUVEC cultures showed an increase of PA compared to the standard control which was set to 100% (P < 0.001); there was no statistically significant differences in the PA of sera obtained pre-surgery from CRC, SC or from healthys individuals on HUVE cells (Table 1). When CRC and SC patients were matched to groups of high and low PA of sera on HUVE cells, taking the 95th percentile value of healthy individuals as a cut-off, nine out of 53 (16.98%) CRC and 3 out of 16 (18.75%) SC patients showed high proliferative values (Fig. 1). Only sera from one CRC patient (1.81%) and one SC patient (6.25%) showed PA values lower than the lowest value observed in the healthy group.


Prognostic value of serum angiogenic activity in colorectal cancer patients.

Gonzalez FJ, Quesada AR, Sevilla I, Baca JJ, Medina MA, Amores J, Diaz JM, Rius-Diaz F, Marques E, Alba E - J. Cell. Mol. Med. (2007 Jan-Feb)

HUVE cell proliferative activity (PA) induced by serum from colorectal cancer patients (CRC, n = 53), gastrointestinal non-malignant diseases patient (SC, n = 16) and healthy individuals (healthy, n = 34). Values represent relative percentages of proliferation compared to control serum (100%). PA in CRC and SC patients and in healthy individuals were increased compared to control values (P < 0.001).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4401225&req=5

fig01: HUVE cell proliferative activity (PA) induced by serum from colorectal cancer patients (CRC, n = 53), gastrointestinal non-malignant diseases patient (SC, n = 16) and healthy individuals (healthy, n = 34). Values represent relative percentages of proliferation compared to control serum (100%). PA in CRC and SC patients and in healthy individuals were increased compared to control values (P < 0.001).
Mentions: Although the addition of CRC, SC or healthy individuals serum to HUVEC cultures showed an increase of PA compared to the standard control which was set to 100% (P < 0.001); there was no statistically significant differences in the PA of sera obtained pre-surgery from CRC, SC or from healthys individuals on HUVE cells (Table 1). When CRC and SC patients were matched to groups of high and low PA of sera on HUVE cells, taking the 95th percentile value of healthy individuals as a cut-off, nine out of 53 (16.98%) CRC and 3 out of 16 (18.75%) SC patients showed high proliferative values (Fig. 1). Only sera from one CRC patient (1.81%) and one SC patient (6.25%) showed PA values lower than the lowest value observed in the healthy group.

Bottom Line: Data were compared with clinical outcome of the patients.Although serum from CRC patients significantly increased the PA of HUVEC, compared to culture control (HUVEC in medium + 10% foetal bovine serum (FBS); P < 0.001); our results indicate that serum PA in CRC patients was similar to that of SC or healthy individuals.Patients with lower PA values after surgery showed a worse outcome that those with higher PA values.

View Article: PubMed Central - PubMed

Affiliation: Medical Oncology Service, Hospital Universitario Virgen de la Victoria, Malaga, Spain. fjgonzalez02@yahoo.es

ABSTRACT
Angiogenesis, resulting from an imbalance between angiogenic activator factors and inhibitors, is required for tumour growth and metastasis. The determination of the circulating concentration of all angiogenic factors (activators and inhibitors) is not feasible at present. We have evaluated diagnostic and prognostic values of the measurement of serum angiogenic activity in colorectal carcinoma (CRC) patients. Serum proliferative activity (PA) on human umbilical vein endothelial cells (HUVEC) in vitro, and serum vascular endothelial growth factor (VEGF) levels were determined by ELISA in 53 patients with primary CRC, 16 subjects with non-neoplastic gastrointestinal disease (SC) and 34 healthy individuals. Data were compared with clinical outcome of the patients. Although serum from CRC patients significantly increased the PA of HUVEC, compared to culture control (HUVEC in medium + 10% foetal bovine serum (FBS); P < 0.001); our results indicate that serum PA in CRC patients was similar to that of SC or healthy individuals. There was no correlation between serum PA and circulating VEGF concentrations. Surgery produced a decrease of PA at 8 hrs after tumour resection in CRC patients compared to pre-surgery values (186 +/- 47 versus 213 +/- 41, P < 0.001). However, an increase in serum VEGF values was observed after surgery (280 [176-450] versus 251 [160-357] pg/ml, P = 0.004). Patients with lower PA values after surgery showed a worse outcome that those with higher PA values. Therefore, this study does not support a diagnostic value for serum angiogenic activity measured by proliferative activity on HUVEC but suggests it could have a prognostic value in CRC patients.

Show MeSH
Related in: MedlinePlus