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Expression of RhoA by inflammatory macrophages and T cells in rat experimental autoimmune neuritis.

Zhang Z, Fauser U, Schluesener HJ - J. Cell. Mol. Med. (2007 Jan-Feb)

Bottom Line: A significant accumulation of RhoA+ cells was observed on Day 12, with a maximum around Day 15, correlating to the clinical severity of EAN.In conclusion, this is the first demonstration of the presence of RhoA in the dorsal/ventral roots of EAN.The time courses and cellular sources of RhoA together with the functions of RhoA indicate that RhoA may function to facilitate macrophage and T-cell infiltration in EAN and therefore could be a potential therapeutic target.

View Article: PubMed Central - PubMed

Affiliation: Institute of Brain Research, University of Tuebingen, Tuebingen, Germany. zhangzhiren@yahoo.com

ABSTRACT
RhoA is one of the best-studied members of Rho GTPases. Experimental autoimmune neuritis (EAN), which is characterized by infiltration of T cells and macrophages into the peripheral nervous system, is an autoantigen-specific T-cell-mediated animal model of human Guillain-Barré Syndrome. In this study, RhoA expression has been investigated in the dorsal/ventral roots of EAN rats by immunohistochemistry. A significant accumulation of RhoA+ cells was observed on Day 12, with a maximum around Day 15, correlating to the clinical severity of EAN. In dorsal/ventral roots of EAN, RhoA+ cells were seen in perivascular areas but also in the parenchyma. Furthermore, double-labelling experiments showed that the major cellular sources of RhoA were reactive macrophages and T cells. In conclusion, this is the first demonstration of the presence of RhoA in the dorsal/ventral roots of EAN. The time courses and cellular sources of RhoA together with the functions of RhoA indicate that RhoA may function to facilitate macrophage and T-cell infiltration in EAN and therefore could be a potential therapeutic target.

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Related in: MedlinePlus

Immunohistochemical labelling of RhoA in spinal cord dorsal and ventral roots of normal and EAN rats. (A) Immunostaining of spinal cord dorsal and ventral roots without the primary antibody as a negative control. (B) RhoA expression was absent in dorsal/ventral roots of normal adult rats. (C) Accumulation of RhoA+ cells was seen in dorsal/ventral roots Day 15 after immunization in EAN. RhoA+ cells accumulated near blood vessels (D), but were also seen in the parenchyma (E). (F–I) RhoA double-labelling experiments. Fifteen days after immunization, most RhoA+ cells (brown) co-expressed reactive microglia/macrophages marker ED1 (F, blue), EMAPII (G, blue) or P2X4R (H, blue). The infiltration of W3/13+ T-lympho-cytes (blue) could be detected and cells co-expressed RhoA (brown) (I). Original magnification: A–I x400.
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fig02: Immunohistochemical labelling of RhoA in spinal cord dorsal and ventral roots of normal and EAN rats. (A) Immunostaining of spinal cord dorsal and ventral roots without the primary antibody as a negative control. (B) RhoA expression was absent in dorsal/ventral roots of normal adult rats. (C) Accumulation of RhoA+ cells was seen in dorsal/ventral roots Day 15 after immunization in EAN. RhoA+ cells accumulated near blood vessels (D), but were also seen in the parenchyma (E). (F–I) RhoA double-labelling experiments. Fifteen days after immunization, most RhoA+ cells (brown) co-expressed reactive microglia/macrophages marker ED1 (F, blue), EMAPII (G, blue) or P2X4R (H, blue). The infiltration of W3/13+ T-lympho-cytes (blue) could be detected and cells co-expressed RhoA (brown) (I). Original magnification: A–I x400.

Mentions: RhoA expression in dorsal and ventral roots of normal rats and EAN rats was studied performed with immunohistochemistry. No immunoreactivity (IR) was detected for the negative control without primary antibody (Fig. 2A). In normal rats, IR of RhoA was occasionally observed in the dorsal and ventral roots (0.8 ± 0.2 per HPF, Fig. 2B).


Expression of RhoA by inflammatory macrophages and T cells in rat experimental autoimmune neuritis.

Zhang Z, Fauser U, Schluesener HJ - J. Cell. Mol. Med. (2007 Jan-Feb)

Immunohistochemical labelling of RhoA in spinal cord dorsal and ventral roots of normal and EAN rats. (A) Immunostaining of spinal cord dorsal and ventral roots without the primary antibody as a negative control. (B) RhoA expression was absent in dorsal/ventral roots of normal adult rats. (C) Accumulation of RhoA+ cells was seen in dorsal/ventral roots Day 15 after immunization in EAN. RhoA+ cells accumulated near blood vessels (D), but were also seen in the parenchyma (E). (F–I) RhoA double-labelling experiments. Fifteen days after immunization, most RhoA+ cells (brown) co-expressed reactive microglia/macrophages marker ED1 (F, blue), EMAPII (G, blue) or P2X4R (H, blue). The infiltration of W3/13+ T-lympho-cytes (blue) could be detected and cells co-expressed RhoA (brown) (I). Original magnification: A–I x400.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4401224&req=5

fig02: Immunohistochemical labelling of RhoA in spinal cord dorsal and ventral roots of normal and EAN rats. (A) Immunostaining of spinal cord dorsal and ventral roots without the primary antibody as a negative control. (B) RhoA expression was absent in dorsal/ventral roots of normal adult rats. (C) Accumulation of RhoA+ cells was seen in dorsal/ventral roots Day 15 after immunization in EAN. RhoA+ cells accumulated near blood vessels (D), but were also seen in the parenchyma (E). (F–I) RhoA double-labelling experiments. Fifteen days after immunization, most RhoA+ cells (brown) co-expressed reactive microglia/macrophages marker ED1 (F, blue), EMAPII (G, blue) or P2X4R (H, blue). The infiltration of W3/13+ T-lympho-cytes (blue) could be detected and cells co-expressed RhoA (brown) (I). Original magnification: A–I x400.
Mentions: RhoA expression in dorsal and ventral roots of normal rats and EAN rats was studied performed with immunohistochemistry. No immunoreactivity (IR) was detected for the negative control without primary antibody (Fig. 2A). In normal rats, IR of RhoA was occasionally observed in the dorsal and ventral roots (0.8 ± 0.2 per HPF, Fig. 2B).

Bottom Line: A significant accumulation of RhoA+ cells was observed on Day 12, with a maximum around Day 15, correlating to the clinical severity of EAN.In conclusion, this is the first demonstration of the presence of RhoA in the dorsal/ventral roots of EAN.The time courses and cellular sources of RhoA together with the functions of RhoA indicate that RhoA may function to facilitate macrophage and T-cell infiltration in EAN and therefore could be a potential therapeutic target.

View Article: PubMed Central - PubMed

Affiliation: Institute of Brain Research, University of Tuebingen, Tuebingen, Germany. zhangzhiren@yahoo.com

ABSTRACT
RhoA is one of the best-studied members of Rho GTPases. Experimental autoimmune neuritis (EAN), which is characterized by infiltration of T cells and macrophages into the peripheral nervous system, is an autoantigen-specific T-cell-mediated animal model of human Guillain-Barré Syndrome. In this study, RhoA expression has been investigated in the dorsal/ventral roots of EAN rats by immunohistochemistry. A significant accumulation of RhoA+ cells was observed on Day 12, with a maximum around Day 15, correlating to the clinical severity of EAN. In dorsal/ventral roots of EAN, RhoA+ cells were seen in perivascular areas but also in the parenchyma. Furthermore, double-labelling experiments showed that the major cellular sources of RhoA were reactive macrophages and T cells. In conclusion, this is the first demonstration of the presence of RhoA in the dorsal/ventral roots of EAN. The time courses and cellular sources of RhoA together with the functions of RhoA indicate that RhoA may function to facilitate macrophage and T-cell infiltration in EAN and therefore could be a potential therapeutic target.

Show MeSH
Related in: MedlinePlus