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Autonomously vascularized cellular constructs in tissue engineering: opening a new perspective for biomedical science.

Polykandriotis E, Arkudas A, Horch RE, Stürzl M, Kneser U - J. Cell. Mol. Med. (2007 Jan-Feb)

Bottom Line: The cell itself is situated at the cross-roads leading to different orders of scale, from molecule to organism and different levels of function, from biochemistry to macrophysiology.Extensive in vitro investigations have dissected a vast amount of cellular phenomena and the role of a number of bioactive substances has been elucidated in the past.Further, recombinant DNA technologies allow modulation of the expression profiles of virtually all kinds of cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic and Hand Surgery, University of Erlangen Medical Center, Erlangen, Germany.

ABSTRACT
In tissue engineering cell cultures play a crucial role besides the matrix materials for the end of substituting lost tissue functions. The cell itself is situated at the cross-roads leading to different orders of scale, from molecule to organism and different levels of function, from biochemistry to macrophysiology. Extensive in vitro investigations have dissected a vast amount of cellular phenomena and the role of a number of bioactive substances has been elucidated in the past. Further, recombinant DNA technologies allow modulation of the expression profiles of virtually all kinds of cells. However, issues of vascularization in vivo limit transferability of these observations and restrict upscaling into clinical applications. Novel in vivo models of vascularization have evolved inspired from reconstructive microsurgical concepts and they encompass axial neovascularization by means of vascular induction. This work represents a brief description of latest developments and potential applications of neovascularization and angiogenesis in tissue engineering.

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Related in: MedlinePlus

Cell survival study with CFDA marker.Transplantation of differentiated primary rat osteoblasts into the prevascularized matrices significantly enhanced initial cell survival (40 × magnification).
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fig05: Cell survival study with CFDA marker.Transplantation of differentiated primary rat osteoblasts into the prevascularized matrices significantly enhanced initial cell survival (40 × magnification).

Mentions: To accomplish the leap from a vascularized solid matrix to a bioartificial unit of bone tissue the matrices were secondarily loaded with primary rat osteoblasts expanded in cell culture and kept in a differentiation medium for 14 days [24]. To elucidate the fate of the cells in vivo, they were marked with a fluorescent dye prior to seeding. Matrices loaded with the same osteoblasts and implanted subcutaneously served as controls. Pre-vascularization by means of a vascular carrier significantly enhanced survival of the cells and increased transplantation efficiency [25] (Fig. 5). Differentiated osteoblasts were able to initiate osteogenesis within the construct. However, full ossification of the matrix could not be accomplished. Other modes of osteoinduction are currently under study.


Autonomously vascularized cellular constructs in tissue engineering: opening a new perspective for biomedical science.

Polykandriotis E, Arkudas A, Horch RE, Stürzl M, Kneser U - J. Cell. Mol. Med. (2007 Jan-Feb)

Cell survival study with CFDA marker.Transplantation of differentiated primary rat osteoblasts into the prevascularized matrices significantly enhanced initial cell survival (40 × magnification).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4401217&req=5

fig05: Cell survival study with CFDA marker.Transplantation of differentiated primary rat osteoblasts into the prevascularized matrices significantly enhanced initial cell survival (40 × magnification).
Mentions: To accomplish the leap from a vascularized solid matrix to a bioartificial unit of bone tissue the matrices were secondarily loaded with primary rat osteoblasts expanded in cell culture and kept in a differentiation medium for 14 days [24]. To elucidate the fate of the cells in vivo, they were marked with a fluorescent dye prior to seeding. Matrices loaded with the same osteoblasts and implanted subcutaneously served as controls. Pre-vascularization by means of a vascular carrier significantly enhanced survival of the cells and increased transplantation efficiency [25] (Fig. 5). Differentiated osteoblasts were able to initiate osteogenesis within the construct. However, full ossification of the matrix could not be accomplished. Other modes of osteoinduction are currently under study.

Bottom Line: The cell itself is situated at the cross-roads leading to different orders of scale, from molecule to organism and different levels of function, from biochemistry to macrophysiology.Extensive in vitro investigations have dissected a vast amount of cellular phenomena and the role of a number of bioactive substances has been elucidated in the past.Further, recombinant DNA technologies allow modulation of the expression profiles of virtually all kinds of cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic and Hand Surgery, University of Erlangen Medical Center, Erlangen, Germany.

ABSTRACT
In tissue engineering cell cultures play a crucial role besides the matrix materials for the end of substituting lost tissue functions. The cell itself is situated at the cross-roads leading to different orders of scale, from molecule to organism and different levels of function, from biochemistry to macrophysiology. Extensive in vitro investigations have dissected a vast amount of cellular phenomena and the role of a number of bioactive substances has been elucidated in the past. Further, recombinant DNA technologies allow modulation of the expression profiles of virtually all kinds of cells. However, issues of vascularization in vivo limit transferability of these observations and restrict upscaling into clinical applications. Novel in vivo models of vascularization have evolved inspired from reconstructive microsurgical concepts and they encompass axial neovascularization by means of vascular induction. This work represents a brief description of latest developments and potential applications of neovascularization and angiogenesis in tissue engineering.

Show MeSH
Related in: MedlinePlus