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Autonomously vascularized cellular constructs in tissue engineering: opening a new perspective for biomedical science.

Polykandriotis E, Arkudas A, Horch RE, Stürzl M, Kneser U - J. Cell. Mol. Med. (2007 Jan-Feb)

Bottom Line: The cell itself is situated at the cross-roads leading to different orders of scale, from molecule to organism and different levels of function, from biochemistry to macrophysiology.Extensive in vitro investigations have dissected a vast amount of cellular phenomena and the role of a number of bioactive substances has been elucidated in the past.Further, recombinant DNA technologies allow modulation of the expression profiles of virtually all kinds of cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic and Hand Surgery, University of Erlangen Medical Center, Erlangen, Germany.

ABSTRACT
In tissue engineering cell cultures play a crucial role besides the matrix materials for the end of substituting lost tissue functions. The cell itself is situated at the cross-roads leading to different orders of scale, from molecule to organism and different levels of function, from biochemistry to macrophysiology. Extensive in vitro investigations have dissected a vast amount of cellular phenomena and the role of a number of bioactive substances has been elucidated in the past. Further, recombinant DNA technologies allow modulation of the expression profiles of virtually all kinds of cells. However, issues of vascularization in vivo limit transferability of these observations and restrict upscaling into clinical applications. Novel in vivo models of vascularization have evolved inspired from reconstructive microsurgical concepts and they encompass axial neovascularization by means of vascular induction. This work represents a brief description of latest developments and potential applications of neovascularization and angiogenesis in tissue engineering.

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Related in: MedlinePlus

Custom-made design of a biogenic processed bovine cancellous bone matrix. A scanning electron image with the discoid matrix displaying a circular notch for optimal accommodation of the AV loop is shown here. The matrix was rendered conductive prior to sputtering by means of minute copper wires. Eight canals for secondary injection of fibrin immobilized osteoblasts were included in the design.
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fig04: Custom-made design of a biogenic processed bovine cancellous bone matrix. A scanning electron image with the discoid matrix displaying a circular notch for optimal accommodation of the AV loop is shown here. The matrix was rendered conductive prior to sputtering by means of minute copper wires. Eight canals for secondary injection of fibrin immobilized osteoblasts were included in the design.

Mentions: A biogenic matrix from bovine spongiosa with porosity between 65% and 80% and a pore size of 400–1000μm rendered acellular and non-antigenic by a standardized procedure was used. The matrix was formed to a design for optimal accommodation of the vascular axis. Different designs were tested for different configurations of the vessels [18, 23]. A central canal with an elliptical cross-section was used for bundle arrangement whereas for loop constructs a design with a circumferencial notch at the periphery of the matrix was applied (Fig. 4). The overall shape was that of a disc 9 mm in diameter and 5 mm thick. Full vascularization of the scaffold could be verified between 6 and 8 weeks post-implantation. The fibrovascular tissue in the matrix displayed a significantly lower ratio of inflammatory elements as opposed to matrices implanted subcutaneously [10]. The ratio of thrombosis in the loop did not significantly differ from ones placed into soft matrices. Under these circumstances the method was shown to be suitable for vascularized bone-like assemblies with primary stability serving as bone substitutes.


Autonomously vascularized cellular constructs in tissue engineering: opening a new perspective for biomedical science.

Polykandriotis E, Arkudas A, Horch RE, Stürzl M, Kneser U - J. Cell. Mol. Med. (2007 Jan-Feb)

Custom-made design of a biogenic processed bovine cancellous bone matrix. A scanning electron image with the discoid matrix displaying a circular notch for optimal accommodation of the AV loop is shown here. The matrix was rendered conductive prior to sputtering by means of minute copper wires. Eight canals for secondary injection of fibrin immobilized osteoblasts were included in the design.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4401217&req=5

fig04: Custom-made design of a biogenic processed bovine cancellous bone matrix. A scanning electron image with the discoid matrix displaying a circular notch for optimal accommodation of the AV loop is shown here. The matrix was rendered conductive prior to sputtering by means of minute copper wires. Eight canals for secondary injection of fibrin immobilized osteoblasts were included in the design.
Mentions: A biogenic matrix from bovine spongiosa with porosity between 65% and 80% and a pore size of 400–1000μm rendered acellular and non-antigenic by a standardized procedure was used. The matrix was formed to a design for optimal accommodation of the vascular axis. Different designs were tested for different configurations of the vessels [18, 23]. A central canal with an elliptical cross-section was used for bundle arrangement whereas for loop constructs a design with a circumferencial notch at the periphery of the matrix was applied (Fig. 4). The overall shape was that of a disc 9 mm in diameter and 5 mm thick. Full vascularization of the scaffold could be verified between 6 and 8 weeks post-implantation. The fibrovascular tissue in the matrix displayed a significantly lower ratio of inflammatory elements as opposed to matrices implanted subcutaneously [10]. The ratio of thrombosis in the loop did not significantly differ from ones placed into soft matrices. Under these circumstances the method was shown to be suitable for vascularized bone-like assemblies with primary stability serving as bone substitutes.

Bottom Line: The cell itself is situated at the cross-roads leading to different orders of scale, from molecule to organism and different levels of function, from biochemistry to macrophysiology.Extensive in vitro investigations have dissected a vast amount of cellular phenomena and the role of a number of bioactive substances has been elucidated in the past.Further, recombinant DNA technologies allow modulation of the expression profiles of virtually all kinds of cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic and Hand Surgery, University of Erlangen Medical Center, Erlangen, Germany.

ABSTRACT
In tissue engineering cell cultures play a crucial role besides the matrix materials for the end of substituting lost tissue functions. The cell itself is situated at the cross-roads leading to different orders of scale, from molecule to organism and different levels of function, from biochemistry to macrophysiology. Extensive in vitro investigations have dissected a vast amount of cellular phenomena and the role of a number of bioactive substances has been elucidated in the past. Further, recombinant DNA technologies allow modulation of the expression profiles of virtually all kinds of cells. However, issues of vascularization in vivo limit transferability of these observations and restrict upscaling into clinical applications. Novel in vivo models of vascularization have evolved inspired from reconstructive microsurgical concepts and they encompass axial neovascularization by means of vascular induction. This work represents a brief description of latest developments and potential applications of neovascularization and angiogenesis in tissue engineering.

Show MeSH
Related in: MedlinePlus