Limits...
Autonomously vascularized cellular constructs in tissue engineering: opening a new perspective for biomedical science.

Polykandriotis E, Arkudas A, Horch RE, Stürzl M, Kneser U - J. Cell. Mol. Med. (2007 Jan-Feb)

Bottom Line: The cell itself is situated at the cross-roads leading to different orders of scale, from molecule to organism and different levels of function, from biochemistry to macrophysiology.Extensive in vitro investigations have dissected a vast amount of cellular phenomena and the role of a number of bioactive substances has been elucidated in the past.Further, recombinant DNA technologies allow modulation of the expression profiles of virtually all kinds of cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic and Hand Surgery, University of Erlangen Medical Center, Erlangen, Germany.

ABSTRACT
In tissue engineering cell cultures play a crucial role besides the matrix materials for the end of substituting lost tissue functions. The cell itself is situated at the cross-roads leading to different orders of scale, from molecule to organism and different levels of function, from biochemistry to macrophysiology. Extensive in vitro investigations have dissected a vast amount of cellular phenomena and the role of a number of bioactive substances has been elucidated in the past. Further, recombinant DNA technologies allow modulation of the expression profiles of virtually all kinds of cells. However, issues of vascularization in vivo limit transferability of these observations and restrict upscaling into clinical applications. Novel in vivo models of vascularization have evolved inspired from reconstructive microsurgical concepts and they encompass axial neovascularization by means of vascular induction. This work represents a brief description of latest developments and potential applications of neovascularization and angiogenesis in tissue engineering.

Show MeSH

Related in: MedlinePlus

Vascularization into the fibrin clot after 2 weeks. Prior to explantation the caudal circulatory system was perfused with an India ink gel for black coloration of the capillary network. Four poly-ethylene stabs serve for stabilization of the AV loop in the isolation chamber.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4401217&req=5

fig03: Vascularization into the fibrin clot after 2 weeks. Prior to explantation the caudal circulatory system was perfused with an India ink gel for black coloration of the capillary network. Four poly-ethylene stabs serve for stabilization of the AV loop in the isolation chamber.

Mentions: The principle was applied on a fibrin gel matrix. Kinetics of angiogenesis were greatly influenced by differences in concentrations of fibrinogen in the clot. In a fibrinogen concentration of 33 mg/ml the onset of neovascularization was delayed until day 12–14 after construction of the loop [22]. In lower concentrations (20 mg/ml) a more rapid vascular growth was accompanied by a marked fibrinolysis with disintegration of the matrix within the first 10 days. Resorption of the fibrin clot was affected by the newly formed capillaries, since in control groups without insertion of a vascular carrier into the isolation chamber, the clots remained intact throughout the length of the experiment. In this investigation we confirmed the capacity of the AV loop to generate a mature vascular network within a fibrin gel (Fig. 3). The process was defined by a gradual substitution of the clot by fibrovascular tissue. Fibrin matrices with a loose structure and low density potentiated angiogenesis but failed to maintain their structural integrity.


Autonomously vascularized cellular constructs in tissue engineering: opening a new perspective for biomedical science.

Polykandriotis E, Arkudas A, Horch RE, Stürzl M, Kneser U - J. Cell. Mol. Med. (2007 Jan-Feb)

Vascularization into the fibrin clot after 2 weeks. Prior to explantation the caudal circulatory system was perfused with an India ink gel for black coloration of the capillary network. Four poly-ethylene stabs serve for stabilization of the AV loop in the isolation chamber.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4401217&req=5

fig03: Vascularization into the fibrin clot after 2 weeks. Prior to explantation the caudal circulatory system was perfused with an India ink gel for black coloration of the capillary network. Four poly-ethylene stabs serve for stabilization of the AV loop in the isolation chamber.
Mentions: The principle was applied on a fibrin gel matrix. Kinetics of angiogenesis were greatly influenced by differences in concentrations of fibrinogen in the clot. In a fibrinogen concentration of 33 mg/ml the onset of neovascularization was delayed until day 12–14 after construction of the loop [22]. In lower concentrations (20 mg/ml) a more rapid vascular growth was accompanied by a marked fibrinolysis with disintegration of the matrix within the first 10 days. Resorption of the fibrin clot was affected by the newly formed capillaries, since in control groups without insertion of a vascular carrier into the isolation chamber, the clots remained intact throughout the length of the experiment. In this investigation we confirmed the capacity of the AV loop to generate a mature vascular network within a fibrin gel (Fig. 3). The process was defined by a gradual substitution of the clot by fibrovascular tissue. Fibrin matrices with a loose structure and low density potentiated angiogenesis but failed to maintain their structural integrity.

Bottom Line: The cell itself is situated at the cross-roads leading to different orders of scale, from molecule to organism and different levels of function, from biochemistry to macrophysiology.Extensive in vitro investigations have dissected a vast amount of cellular phenomena and the role of a number of bioactive substances has been elucidated in the past.Further, recombinant DNA technologies allow modulation of the expression profiles of virtually all kinds of cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic and Hand Surgery, University of Erlangen Medical Center, Erlangen, Germany.

ABSTRACT
In tissue engineering cell cultures play a crucial role besides the matrix materials for the end of substituting lost tissue functions. The cell itself is situated at the cross-roads leading to different orders of scale, from molecule to organism and different levels of function, from biochemistry to macrophysiology. Extensive in vitro investigations have dissected a vast amount of cellular phenomena and the role of a number of bioactive substances has been elucidated in the past. Further, recombinant DNA technologies allow modulation of the expression profiles of virtually all kinds of cells. However, issues of vascularization in vivo limit transferability of these observations and restrict upscaling into clinical applications. Novel in vivo models of vascularization have evolved inspired from reconstructive microsurgical concepts and they encompass axial neovascularization by means of vascular induction. This work represents a brief description of latest developments and potential applications of neovascularization and angiogenesis in tissue engineering.

Show MeSH
Related in: MedlinePlus