Limits...
Autonomously vascularized cellular constructs in tissue engineering: opening a new perspective for biomedical science.

Polykandriotis E, Arkudas A, Horch RE, Stürzl M, Kneser U - J. Cell. Mol. Med. (2007 Jan-Feb)

Bottom Line: The cell itself is situated at the cross-roads leading to different orders of scale, from molecule to organism and different levels of function, from biochemistry to macrophysiology.Extensive in vitro investigations have dissected a vast amount of cellular phenomena and the role of a number of bioactive substances has been elucidated in the past.Further, recombinant DNA technologies allow modulation of the expression profiles of virtually all kinds of cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic and Hand Surgery, University of Erlangen Medical Center, Erlangen, Germany.

ABSTRACT
In tissue engineering cell cultures play a crucial role besides the matrix materials for the end of substituting lost tissue functions. The cell itself is situated at the cross-roads leading to different orders of scale, from molecule to organism and different levels of function, from biochemistry to macrophysiology. Extensive in vitro investigations have dissected a vast amount of cellular phenomena and the role of a number of bioactive substances has been elucidated in the past. Further, recombinant DNA technologies allow modulation of the expression profiles of virtually all kinds of cells. However, issues of vascularization in vivo limit transferability of these observations and restrict upscaling into clinical applications. Novel in vivo models of vascularization have evolved inspired from reconstructive microsurgical concepts and they encompass axial neovascularization by means of vascular induction. This work represents a brief description of latest developments and potential applications of neovascularization and angiogenesis in tissue engineering.

Show MeSH

Related in: MedlinePlus

Schematic representation of the isolation chamber. It is comprised by a base plate (B) (diameter: 15 mm), under a cylindrical shell (height 6 mm × diameter 12 mm) (C) and an upper lid (L) (height: 2 mm × diameter: 14 mm). At the sides there are perforations for fixation of the chamber on the fascia of the medial musculature of the medial thigh.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4401217&req=5

fig02: Schematic representation of the isolation chamber. It is comprised by a base plate (B) (diameter: 15 mm), under a cylindrical shell (height 6 mm × diameter 12 mm) (C) and an upper lid (L) (height: 2 mm × diameter: 14 mm). At the sides there are perforations for fixation of the chamber on the fascia of the medial musculature of the medial thigh.

Mentions: An isolation chamber separates the fibrovascular construct from the organism with exception of the arteriovenous pedicle entering and exiting the chamber from an aperture at the proximal pole. The design was developed in rat cadaver studies and during long-term experiments [19]. The chamber is made of medical grade Teflon; a biologically inert material amenable to sterilization. It is comprised by a base plate (diameter: 15 mm), under a cylindrical shell (height 6 mm diameter × 12 mm) and an upper cup (height: 2 mm × diameter: 14 mm) (Fig. 2). The basal plate has two peripheral perforations for stabilization on the fascia of the medial musculature of the thigh. The cup has a spherical form so as to minimize the risk of skin perforations in long-term investigations. A different design with ancoring stabs was applied for gel matrices to avoid dislocation of the loop outside the chamber.


Autonomously vascularized cellular constructs in tissue engineering: opening a new perspective for biomedical science.

Polykandriotis E, Arkudas A, Horch RE, Stürzl M, Kneser U - J. Cell. Mol. Med. (2007 Jan-Feb)

Schematic representation of the isolation chamber. It is comprised by a base plate (B) (diameter: 15 mm), under a cylindrical shell (height 6 mm × diameter 12 mm) (C) and an upper lid (L) (height: 2 mm × diameter: 14 mm). At the sides there are perforations for fixation of the chamber on the fascia of the medial musculature of the medial thigh.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4401217&req=5

fig02: Schematic representation of the isolation chamber. It is comprised by a base plate (B) (diameter: 15 mm), under a cylindrical shell (height 6 mm × diameter 12 mm) (C) and an upper lid (L) (height: 2 mm × diameter: 14 mm). At the sides there are perforations for fixation of the chamber on the fascia of the medial musculature of the medial thigh.
Mentions: An isolation chamber separates the fibrovascular construct from the organism with exception of the arteriovenous pedicle entering and exiting the chamber from an aperture at the proximal pole. The design was developed in rat cadaver studies and during long-term experiments [19]. The chamber is made of medical grade Teflon; a biologically inert material amenable to sterilization. It is comprised by a base plate (diameter: 15 mm), under a cylindrical shell (height 6 mm diameter × 12 mm) and an upper cup (height: 2 mm × diameter: 14 mm) (Fig. 2). The basal plate has two peripheral perforations for stabilization on the fascia of the medial musculature of the thigh. The cup has a spherical form so as to minimize the risk of skin perforations in long-term investigations. A different design with ancoring stabs was applied for gel matrices to avoid dislocation of the loop outside the chamber.

Bottom Line: The cell itself is situated at the cross-roads leading to different orders of scale, from molecule to organism and different levels of function, from biochemistry to macrophysiology.Extensive in vitro investigations have dissected a vast amount of cellular phenomena and the role of a number of bioactive substances has been elucidated in the past.Further, recombinant DNA technologies allow modulation of the expression profiles of virtually all kinds of cells.

View Article: PubMed Central - PubMed

Affiliation: Department of Plastic and Hand Surgery, University of Erlangen Medical Center, Erlangen, Germany.

ABSTRACT
In tissue engineering cell cultures play a crucial role besides the matrix materials for the end of substituting lost tissue functions. The cell itself is situated at the cross-roads leading to different orders of scale, from molecule to organism and different levels of function, from biochemistry to macrophysiology. Extensive in vitro investigations have dissected a vast amount of cellular phenomena and the role of a number of bioactive substances has been elucidated in the past. Further, recombinant DNA technologies allow modulation of the expression profiles of virtually all kinds of cells. However, issues of vascularization in vivo limit transferability of these observations and restrict upscaling into clinical applications. Novel in vivo models of vascularization have evolved inspired from reconstructive microsurgical concepts and they encompass axial neovascularization by means of vascular induction. This work represents a brief description of latest developments and potential applications of neovascularization and angiogenesis in tissue engineering.

Show MeSH
Related in: MedlinePlus