Limits...
Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is associated to a down-regulation in the expression of endogenous endostatin.

Mangieri D, Nico B, Benagiano V, De Giorgis M, Vacca A, Ribatti D - J. Cell. Mol. Med. (2008)

Bottom Line: We have attempted a fine characterization of the angiogenic response induced by multiple myeloma endothelial cells (MMEC) by using the chick embryo chorioallantoic membrane (CAM) assay and by reverse transcriptase-polymerase chain reaction (RT-PCR).Results showed that in the CAM assay MMEC induced an angiogenic response comparable to that of a well-known angiogenic cytokine, namely fibroblast growth factor-2 (FGF-2), while RT-PCR demonstrated that the expression of endostatin mRNA detected in MM treated CAM was significantly lower respect to control CAM.These data suggest that angiogenic switch in MM may involve loss of an endogenous angiogenesis inhibitor, such as endostatin.

View Article: PubMed Central - PubMed

Affiliation: Department of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy.

ABSTRACT
We have attempted a fine characterization of the angiogenic response induced by multiple myeloma endothelial cells (MMEC) by using the chick embryo chorioallantoic membrane (CAM) assay and by reverse transcriptase-polymerase chain reaction (RT-PCR). Results showed that in the CAM assay MMEC induced an angiogenic response comparable to that of a well-known angiogenic cytokine, namely fibroblast growth factor-2 (FGF-2), while RT-PCR demonstrated that the expression of endostatin mRNA detected in MM treated CAM was significantly lower respect to control CAM. These data suggest that angiogenic switch in MM may involve loss of an endogenous angiogenesis inhibitor, such as endostatin.

Show MeSH

Related in: MedlinePlus

Immunoblotting analysis and quantification after Western blot analysis of endostatin expression in CAM treated with EC obtained from 3 MM and 3 MGUS patients. The error band represents the standard deviation of three experiments.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4401142&req=5

fig03: Immunoblotting analysis and quantification after Western blot analysis of endostatin expression in CAM treated with EC obtained from 3 MM and 3 MGUS patients. The error band represents the standard deviation of three experiments.

Mentions: Western blot analyses were also performed to study whether the expression of endostatin changes in MMEC and MGUSEC treated CAM respect to control. As shown in Fig. 3, according to RT-PCR quantification, endostatin was significantly lower also at the protein level in both MGUSEC and MMEC treated CAM respect to control CAM (MGUS OD: 97.14±10 and MM OD: 52.3±13.6 versus CTRL OD: 158.71±5.52; P<0.05).


Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is associated to a down-regulation in the expression of endogenous endostatin.

Mangieri D, Nico B, Benagiano V, De Giorgis M, Vacca A, Ribatti D - J. Cell. Mol. Med. (2008)

Immunoblotting analysis and quantification after Western blot analysis of endostatin expression in CAM treated with EC obtained from 3 MM and 3 MGUS patients. The error band represents the standard deviation of three experiments.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4401142&req=5

fig03: Immunoblotting analysis and quantification after Western blot analysis of endostatin expression in CAM treated with EC obtained from 3 MM and 3 MGUS patients. The error band represents the standard deviation of three experiments.
Mentions: Western blot analyses were also performed to study whether the expression of endostatin changes in MMEC and MGUSEC treated CAM respect to control. As shown in Fig. 3, according to RT-PCR quantification, endostatin was significantly lower also at the protein level in both MGUSEC and MMEC treated CAM respect to control CAM (MGUS OD: 97.14±10 and MM OD: 52.3±13.6 versus CTRL OD: 158.71±5.52; P<0.05).

Bottom Line: We have attempted a fine characterization of the angiogenic response induced by multiple myeloma endothelial cells (MMEC) by using the chick embryo chorioallantoic membrane (CAM) assay and by reverse transcriptase-polymerase chain reaction (RT-PCR).Results showed that in the CAM assay MMEC induced an angiogenic response comparable to that of a well-known angiogenic cytokine, namely fibroblast growth factor-2 (FGF-2), while RT-PCR demonstrated that the expression of endostatin mRNA detected in MM treated CAM was significantly lower respect to control CAM.These data suggest that angiogenic switch in MM may involve loss of an endogenous angiogenesis inhibitor, such as endostatin.

View Article: PubMed Central - PubMed

Affiliation: Department of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy.

ABSTRACT
We have attempted a fine characterization of the angiogenic response induced by multiple myeloma endothelial cells (MMEC) by using the chick embryo chorioallantoic membrane (CAM) assay and by reverse transcriptase-polymerase chain reaction (RT-PCR). Results showed that in the CAM assay MMEC induced an angiogenic response comparable to that of a well-known angiogenic cytokine, namely fibroblast growth factor-2 (FGF-2), while RT-PCR demonstrated that the expression of endostatin mRNA detected in MM treated CAM was significantly lower respect to control CAM. These data suggest that angiogenic switch in MM may involve loss of an endogenous angiogenesis inhibitor, such as endostatin.

Show MeSH
Related in: MedlinePlus