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Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is associated to a down-regulation in the expression of endogenous endostatin.

Mangieri D, Nico B, Benagiano V, De Giorgis M, Vacca A, Ribatti D - J. Cell. Mol. Med. (2008)

Bottom Line: We have attempted a fine characterization of the angiogenic response induced by multiple myeloma endothelial cells (MMEC) by using the chick embryo chorioallantoic membrane (CAM) assay and by reverse transcriptase-polymerase chain reaction (RT-PCR).Results showed that in the CAM assay MMEC induced an angiogenic response comparable to that of a well-known angiogenic cytokine, namely fibroblast growth factor-2 (FGF-2), while RT-PCR demonstrated that the expression of endostatin mRNA detected in MM treated CAM was significantly lower respect to control CAM.These data suggest that angiogenic switch in MM may involve loss of an endogenous angiogenesis inhibitor, such as endostatin.

View Article: PubMed Central - PubMed

Affiliation: Department of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy.

ABSTRACT
We have attempted a fine characterization of the angiogenic response induced by multiple myeloma endothelial cells (MMEC) by using the chick embryo chorioallantoic membrane (CAM) assay and by reverse transcriptase-polymerase chain reaction (RT-PCR). Results showed that in the CAM assay MMEC induced an angiogenic response comparable to that of a well-known angiogenic cytokine, namely fibroblast growth factor-2 (FGF-2), while RT-PCR demonstrated that the expression of endostatin mRNA detected in MM treated CAM was significantly lower respect to control CAM. These data suggest that angiogenic switch in MM may involve loss of an endogenous angiogenesis inhibitor, such as endostatin.

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Expression levels of mRNA coding for VEGF, FGF-2, Ang-1, HIF1α and endostatin evaluated by semi-quantitative RT-PCR. Transcript levels from the CAM assay are referred to EC obtained from 6 MM and 6 MMGUS patients and the error band represents the standard deviation of six experiments.
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fig02: Expression levels of mRNA coding for VEGF, FGF-2, Ang-1, HIF1α and endostatin evaluated by semi-quantitative RT-PCR. Transcript levels from the CAM assay are referred to EC obtained from 6 MM and 6 MMGUS patients and the error band represents the standard deviation of six experiments.

Mentions: As shown in Fig. 2, treatment of CAM with MMEC and MGUSEC did not produce significant difference in the expression of pro-angiogenic factors mRNA in comparison with control CAM treated with medium alone. Moreover, no statistical difference was found in VEGF, FGF-2, Ang-1, HIF-1α and endostatin mRNA content between MM and MGUS treated CAM (P<0.05). In contrast, the expression of endostatin mRNA detected in both MGUS and MM treated CAM was significantly lower respect to control CAM (MGUS OD:120.1±13 and MM OD:48.3±19.6 versus CTRL OD:205.84 ± 19.6; P<0.05).


Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is associated to a down-regulation in the expression of endogenous endostatin.

Mangieri D, Nico B, Benagiano V, De Giorgis M, Vacca A, Ribatti D - J. Cell. Mol. Med. (2008)

Expression levels of mRNA coding for VEGF, FGF-2, Ang-1, HIF1α and endostatin evaluated by semi-quantitative RT-PCR. Transcript levels from the CAM assay are referred to EC obtained from 6 MM and 6 MMGUS patients and the error band represents the standard deviation of six experiments.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4401142&req=5

fig02: Expression levels of mRNA coding for VEGF, FGF-2, Ang-1, HIF1α and endostatin evaluated by semi-quantitative RT-PCR. Transcript levels from the CAM assay are referred to EC obtained from 6 MM and 6 MMGUS patients and the error band represents the standard deviation of six experiments.
Mentions: As shown in Fig. 2, treatment of CAM with MMEC and MGUSEC did not produce significant difference in the expression of pro-angiogenic factors mRNA in comparison with control CAM treated with medium alone. Moreover, no statistical difference was found in VEGF, FGF-2, Ang-1, HIF-1α and endostatin mRNA content between MM and MGUS treated CAM (P<0.05). In contrast, the expression of endostatin mRNA detected in both MGUS and MM treated CAM was significantly lower respect to control CAM (MGUS OD:120.1±13 and MM OD:48.3±19.6 versus CTRL OD:205.84 ± 19.6; P<0.05).

Bottom Line: We have attempted a fine characterization of the angiogenic response induced by multiple myeloma endothelial cells (MMEC) by using the chick embryo chorioallantoic membrane (CAM) assay and by reverse transcriptase-polymerase chain reaction (RT-PCR).Results showed that in the CAM assay MMEC induced an angiogenic response comparable to that of a well-known angiogenic cytokine, namely fibroblast growth factor-2 (FGF-2), while RT-PCR demonstrated that the expression of endostatin mRNA detected in MM treated CAM was significantly lower respect to control CAM.These data suggest that angiogenic switch in MM may involve loss of an endogenous angiogenesis inhibitor, such as endostatin.

View Article: PubMed Central - PubMed

Affiliation: Department of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy.

ABSTRACT
We have attempted a fine characterization of the angiogenic response induced by multiple myeloma endothelial cells (MMEC) by using the chick embryo chorioallantoic membrane (CAM) assay and by reverse transcriptase-polymerase chain reaction (RT-PCR). Results showed that in the CAM assay MMEC induced an angiogenic response comparable to that of a well-known angiogenic cytokine, namely fibroblast growth factor-2 (FGF-2), while RT-PCR demonstrated that the expression of endostatin mRNA detected in MM treated CAM was significantly lower respect to control CAM. These data suggest that angiogenic switch in MM may involve loss of an endogenous angiogenesis inhibitor, such as endostatin.

Show MeSH
Related in: MedlinePlus