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Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is associated to a down-regulation in the expression of endogenous endostatin.

Mangieri D, Nico B, Benagiano V, De Giorgis M, Vacca A, Ribatti D - J. Cell. Mol. Med. (2008)

Bottom Line: We have attempted a fine characterization of the angiogenic response induced by multiple myeloma endothelial cells (MMEC) by using the chick embryo chorioallantoic membrane (CAM) assay and by reverse transcriptase-polymerase chain reaction (RT-PCR).Results showed that in the CAM assay MMEC induced an angiogenic response comparable to that of a well-known angiogenic cytokine, namely fibroblast growth factor-2 (FGF-2), while RT-PCR demonstrated that the expression of endostatin mRNA detected in MM treated CAM was significantly lower respect to control CAM.These data suggest that angiogenic switch in MM may involve loss of an endogenous angiogenesis inhibitor, such as endostatin.

View Article: PubMed Central - PubMed

Affiliation: Department of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy.

ABSTRACT
We have attempted a fine characterization of the angiogenic response induced by multiple myeloma endothelial cells (MMEC) by using the chick embryo chorioallantoic membrane (CAM) assay and by reverse transcriptase-polymerase chain reaction (RT-PCR). Results showed that in the CAM assay MMEC induced an angiogenic response comparable to that of a well-known angiogenic cytokine, namely fibroblast growth factor-2 (FGF-2), while RT-PCR demonstrated that the expression of endostatin mRNA detected in MM treated CAM was significantly lower respect to control CAM. These data suggest that angiogenic switch in MM may involve loss of an endogenous angiogenesis inhibitor, such as endostatin.

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Related in: MedlinePlus

Angiogenic activity in the chorioallantoic membrane (CAM) assay. Gelatin sponges loaded with FGF-2 (A), MMEC (B), RPMI-1640 (C) and MGUSEC (D) were implanted on top of the CAM on day 8. Macroscopic view of the CAM on day 12 shows numerous allantoic vessels converging like spokes toward the sponge in A and B, while few allantoic vessels are recognizable in C and D. Original magnification: A–D, x 50.
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fig01: Angiogenic activity in the chorioallantoic membrane (CAM) assay. Gelatin sponges loaded with FGF-2 (A), MMEC (B), RPMI-1640 (C) and MGUSEC (D) were implanted on top of the CAM on day 8. Macroscopic view of the CAM on day 12 shows numerous allantoic vessels converging like spokes toward the sponge in A and B, while few allantoic vessels are recognizable in C and D. Original magnification: A–D, x 50.

Mentions: The ability of MMEC and MGUSEC to induce an angiogenic response in vivo was assessed with the CAM-gelatin sponge assay. Vessels entering the sponge were recognized macroscopically and counted (Table 1 and Fig. 1). CAM implanted with the medium containing FGF-2 or MMEC gave significantly higher vessel counts and numerous allantoic vessels converging like spokes toward the sponges were recognizable (Table 1 and Fig. 1). By contrast, when the sponges were loaded with the medium alone and with MGUSEC, physiologic angiogenesis was observed in the form of few allantoic vessels partly around and partly converging toward the sponge (Table 1 and Fig. 1).


Angiogenic activity of multiple myeloma endothelial cells in vivo in the chick embryo chorioallantoic membrane assay is associated to a down-regulation in the expression of endogenous endostatin.

Mangieri D, Nico B, Benagiano V, De Giorgis M, Vacca A, Ribatti D - J. Cell. Mol. Med. (2008)

Angiogenic activity in the chorioallantoic membrane (CAM) assay. Gelatin sponges loaded with FGF-2 (A), MMEC (B), RPMI-1640 (C) and MGUSEC (D) were implanted on top of the CAM on day 8. Macroscopic view of the CAM on day 12 shows numerous allantoic vessels converging like spokes toward the sponge in A and B, while few allantoic vessels are recognizable in C and D. Original magnification: A–D, x 50.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4401142&req=5

fig01: Angiogenic activity in the chorioallantoic membrane (CAM) assay. Gelatin sponges loaded with FGF-2 (A), MMEC (B), RPMI-1640 (C) and MGUSEC (D) were implanted on top of the CAM on day 8. Macroscopic view of the CAM on day 12 shows numerous allantoic vessels converging like spokes toward the sponge in A and B, while few allantoic vessels are recognizable in C and D. Original magnification: A–D, x 50.
Mentions: The ability of MMEC and MGUSEC to induce an angiogenic response in vivo was assessed with the CAM-gelatin sponge assay. Vessels entering the sponge were recognized macroscopically and counted (Table 1 and Fig. 1). CAM implanted with the medium containing FGF-2 or MMEC gave significantly higher vessel counts and numerous allantoic vessels converging like spokes toward the sponges were recognizable (Table 1 and Fig. 1). By contrast, when the sponges were loaded with the medium alone and with MGUSEC, physiologic angiogenesis was observed in the form of few allantoic vessels partly around and partly converging toward the sponge (Table 1 and Fig. 1).

Bottom Line: We have attempted a fine characterization of the angiogenic response induced by multiple myeloma endothelial cells (MMEC) by using the chick embryo chorioallantoic membrane (CAM) assay and by reverse transcriptase-polymerase chain reaction (RT-PCR).Results showed that in the CAM assay MMEC induced an angiogenic response comparable to that of a well-known angiogenic cytokine, namely fibroblast growth factor-2 (FGF-2), while RT-PCR demonstrated that the expression of endostatin mRNA detected in MM treated CAM was significantly lower respect to control CAM.These data suggest that angiogenic switch in MM may involve loss of an endogenous angiogenesis inhibitor, such as endostatin.

View Article: PubMed Central - PubMed

Affiliation: Department of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy.

ABSTRACT
We have attempted a fine characterization of the angiogenic response induced by multiple myeloma endothelial cells (MMEC) by using the chick embryo chorioallantoic membrane (CAM) assay and by reverse transcriptase-polymerase chain reaction (RT-PCR). Results showed that in the CAM assay MMEC induced an angiogenic response comparable to that of a well-known angiogenic cytokine, namely fibroblast growth factor-2 (FGF-2), while RT-PCR demonstrated that the expression of endostatin mRNA detected in MM treated CAM was significantly lower respect to control CAM. These data suggest that angiogenic switch in MM may involve loss of an endogenous angiogenesis inhibitor, such as endostatin.

Show MeSH
Related in: MedlinePlus