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Fetal stem cells from extra-embryonic tissues: do not discard.

Marcus AJ, Woodbury D - J. Cell. Mol. Med. (2008)

Bottom Line: Extra-embryonic tissues are large, potentially increasing the number of stem cells that can be extracted.Lastly, the generation and sequestration of cells that form extra-embryonic tissues occurs early in development and may endow resident stem cell populations with enhanced potency.In this review we summarize recent work examining the plasticity and clinical potential of fetal stem cells isolated from extra-embryonic tissues.

View Article: PubMed Central - PubMed

Affiliation: The Ira B. Black Center for Stem Cell Research and the Department of Neuroscience and Cell Biology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, NJ 08854-5635, USA. marcusak@umdnj.edu

ABSTRACT
Stem cells hold promise to treat diseases currently unapproachable, including Parkinson's disease, liver disease and diabetes. Seminal research has demonstrated the ability of embryonic and adult stem cells to differentiate into clinically useful cell types in vitro and in vivo. More recently, the potential of fetal stem cells derived from extra-embryonic tissues has been investigated. Fetal stem cells are particularly appealing for clinical applications. The cells are readily isolated from tissues normally discarded at birth, avoiding ethical concerns that plague the isolation embryonic stem cells. Extra-embryonic tissues are large, potentially increasing the number of stem cells that can be extracted. Lastly, the generation and sequestration of cells that form extra-embryonic tissues occurs early in development and may endow resident stem cell populations with enhanced potency. In this review we summarize recent work examining the plasticity and clinical potential of fetal stem cells isolated from extra-embryonic tissues.

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Related in: MedlinePlus

Low-density lipoprotein (LDL) uptake by placenta-derived multipotent cell (PDMC)-derived hepatocyte-like cells. Undifferentiated cells (A) and PDMCs cultured on poly-L-lysine-coated dishes with hepatocyte growth factor (HGF) and fibroblast growth factor-4 (FGF-4) for 14 days (B) were incubated with Dil-acil-LDL. The labeled cells were counter-stained with 4’,6-diamino-2-phenylindole and photographed by fluorescence microscopy (magnification x200). Periodic acid-Schiff (PAS) staining of undifferentiated and differentiated PDMCs. PDMCs were cultured on poly-L-lysine-coated dishes with HGF and FGF-4 for 14 days. PAS staining was described in Materials and methods. (C) Undifferentiated PDMCs. (D) PDMC-derived hepatocyte-like cells (magnification x100). Reprinted with permission Stem Cells Vol. 24 No. 7 July 2006, pp.1759–68.
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fig04: Low-density lipoprotein (LDL) uptake by placenta-derived multipotent cell (PDMC)-derived hepatocyte-like cells. Undifferentiated cells (A) and PDMCs cultured on poly-L-lysine-coated dishes with hepatocyte growth factor (HGF) and fibroblast growth factor-4 (FGF-4) for 14 days (B) were incubated with Dil-acil-LDL. The labeled cells were counter-stained with 4’,6-diamino-2-phenylindole and photographed by fluorescence microscopy (magnification x200). Periodic acid-Schiff (PAS) staining of undifferentiated and differentiated PDMCs. PDMCs were cultured on poly-L-lysine-coated dishes with HGF and FGF-4 for 14 days. PAS staining was described in Materials and methods. (C) Undifferentiated PDMCs. (D) PDMC-derived hepatocyte-like cells (magnification x100). Reprinted with permission Stem Cells Vol. 24 No. 7 July 2006, pp.1759–68.

Mentions: Functional properties of mature hepatocytes were also present in the differentiated cells, including internalization of low-density lipoprotein (LDL) (Fig. 4). Control cultures of PDSCs not exposed to differentiation cues were incapable of performing this task. Similarly, glycogen storage was only detected in the differentiated cells.


Fetal stem cells from extra-embryonic tissues: do not discard.

Marcus AJ, Woodbury D - J. Cell. Mol. Med. (2008)

Low-density lipoprotein (LDL) uptake by placenta-derived multipotent cell (PDMC)-derived hepatocyte-like cells. Undifferentiated cells (A) and PDMCs cultured on poly-L-lysine-coated dishes with hepatocyte growth factor (HGF) and fibroblast growth factor-4 (FGF-4) for 14 days (B) were incubated with Dil-acil-LDL. The labeled cells were counter-stained with 4’,6-diamino-2-phenylindole and photographed by fluorescence microscopy (magnification x200). Periodic acid-Schiff (PAS) staining of undifferentiated and differentiated PDMCs. PDMCs were cultured on poly-L-lysine-coated dishes with HGF and FGF-4 for 14 days. PAS staining was described in Materials and methods. (C) Undifferentiated PDMCs. (D) PDMC-derived hepatocyte-like cells (magnification x100). Reprinted with permission Stem Cells Vol. 24 No. 7 July 2006, pp.1759–68.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4401124&req=5

fig04: Low-density lipoprotein (LDL) uptake by placenta-derived multipotent cell (PDMC)-derived hepatocyte-like cells. Undifferentiated cells (A) and PDMCs cultured on poly-L-lysine-coated dishes with hepatocyte growth factor (HGF) and fibroblast growth factor-4 (FGF-4) for 14 days (B) were incubated with Dil-acil-LDL. The labeled cells were counter-stained with 4’,6-diamino-2-phenylindole and photographed by fluorescence microscopy (magnification x200). Periodic acid-Schiff (PAS) staining of undifferentiated and differentiated PDMCs. PDMCs were cultured on poly-L-lysine-coated dishes with HGF and FGF-4 for 14 days. PAS staining was described in Materials and methods. (C) Undifferentiated PDMCs. (D) PDMC-derived hepatocyte-like cells (magnification x100). Reprinted with permission Stem Cells Vol. 24 No. 7 July 2006, pp.1759–68.
Mentions: Functional properties of mature hepatocytes were also present in the differentiated cells, including internalization of low-density lipoprotein (LDL) (Fig. 4). Control cultures of PDSCs not exposed to differentiation cues were incapable of performing this task. Similarly, glycogen storage was only detected in the differentiated cells.

Bottom Line: Extra-embryonic tissues are large, potentially increasing the number of stem cells that can be extracted.Lastly, the generation and sequestration of cells that form extra-embryonic tissues occurs early in development and may endow resident stem cell populations with enhanced potency.In this review we summarize recent work examining the plasticity and clinical potential of fetal stem cells isolated from extra-embryonic tissues.

View Article: PubMed Central - PubMed

Affiliation: The Ira B. Black Center for Stem Cell Research and the Department of Neuroscience and Cell Biology, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School, Piscataway, NJ 08854-5635, USA. marcusak@umdnj.edu

ABSTRACT
Stem cells hold promise to treat diseases currently unapproachable, including Parkinson's disease, liver disease and diabetes. Seminal research has demonstrated the ability of embryonic and adult stem cells to differentiate into clinically useful cell types in vitro and in vivo. More recently, the potential of fetal stem cells derived from extra-embryonic tissues has been investigated. Fetal stem cells are particularly appealing for clinical applications. The cells are readily isolated from tissues normally discarded at birth, avoiding ethical concerns that plague the isolation embryonic stem cells. Extra-embryonic tissues are large, potentially increasing the number of stem cells that can be extracted. Lastly, the generation and sequestration of cells that form extra-embryonic tissues occurs early in development and may endow resident stem cell populations with enhanced potency. In this review we summarize recent work examining the plasticity and clinical potential of fetal stem cells isolated from extra-embryonic tissues.

Show MeSH
Related in: MedlinePlus