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Activity Assay of Glutathione S-Transferase (GSTs) Enzyme as a Diagnostic Biomarker for Liver Hydatid Cyst in Vitro.

Moatamedi Pour L, Farahnak A, Molaei Rad M, Golmohamadi T, Eshraghian M - Iran. J. Public Health (2014)

Bottom Line: To determine significant difference between two groups, two-sample t-test was performed.T-test analysis showed GST activity of parasite was lower than healthy liver tissue.SDS-PAGE showed GST protein bands with 24 kDa in parasite samples and25 kDa in liver tissues.

View Article: PubMed Central - PubMed

Affiliation: 1. Dept. of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Iran.

ABSTRACT

Background: The aim of this study was to detect the Glutathione S-Transferase(GST) enzyme activity of healthy / cystic liver as a diagnostic biomarker for hydatidosis. In order to compare with liver tissue, the level of the GSTs enzyme activity of parasite was also determined.

Methods: Parasites were collected from sheep liver tissue with hydatid cysts at a local abattoir and washed with PBS buffer. Collected parasites and liver tissues were sonicated or homogenized respectively. Extract solution samples were centrifuged and stored at - 20°C. GST enzyme activities were measured in the extract of parasite and liver tissue samples (healthy and infected livers). Protein amounts and protein bands were detected using Bradford and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) methods respectively. To determine significant difference between two groups, two-sample t-test was performed.

Results: GST specific activities of healthy / infected livers and parasites were estimated 304, 1297 and 146 U/ml/mg respectively. Significant higher GST specific activities in cystic liver than healthy liver was observed (P <0.05). T-test analysis showed GST activity of parasite was lower than healthy liver tissue. SDS-PAGE showed GST protein bands with 24 kDa in parasite samples and25 kDa in liver tissues.

Conclusion: GST activity incystic liver tissue could be concerned as a biomarker for hydatid cyst diagnosis with other hydatid disease parameters.

No MeSH data available.


Related in: MedlinePlus

Molecular weight of proteins (kDa) from the extracts of parasite and liver tissues in SDS-PAGE. Lanes 1-3 are healthy liver samples. Lanes 4-6 are cystic liver samples. Lanes 7-9 are parasite samples (Protein marker, peqGOLD I, Lot-No. 64072)
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Figure 1: Molecular weight of proteins (kDa) from the extracts of parasite and liver tissues in SDS-PAGE. Lanes 1-3 are healthy liver samples. Lanes 4-6 are cystic liver samples. Lanes 7-9 are parasite samples (Protein marker, peqGOLD I, Lot-No. 64072)

Mentions: Extract samples of protoscolices, healthy and cystic liver tissues were analyzed by SDS-PAGE electrophoresis and the results are shown in Fig. 1. SDS-PAGE gel shows GST protein bands with 24kDa in parasite and 25kDa in healthy liver samples. Similar cross protein bands is observing in parasite and liver sample but has been not recorded by databse. Identified proteins are presented in table 2, 3.


Activity Assay of Glutathione S-Transferase (GSTs) Enzyme as a Diagnostic Biomarker for Liver Hydatid Cyst in Vitro.

Moatamedi Pour L, Farahnak A, Molaei Rad M, Golmohamadi T, Eshraghian M - Iran. J. Public Health (2014)

Molecular weight of proteins (kDa) from the extracts of parasite and liver tissues in SDS-PAGE. Lanes 1-3 are healthy liver samples. Lanes 4-6 are cystic liver samples. Lanes 7-9 are parasite samples (Protein marker, peqGOLD I, Lot-No. 64072)
© Copyright Policy - open-access
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4401064&req=5

Figure 1: Molecular weight of proteins (kDa) from the extracts of parasite and liver tissues in SDS-PAGE. Lanes 1-3 are healthy liver samples. Lanes 4-6 are cystic liver samples. Lanes 7-9 are parasite samples (Protein marker, peqGOLD I, Lot-No. 64072)
Mentions: Extract samples of protoscolices, healthy and cystic liver tissues were analyzed by SDS-PAGE electrophoresis and the results are shown in Fig. 1. SDS-PAGE gel shows GST protein bands with 24kDa in parasite and 25kDa in healthy liver samples. Similar cross protein bands is observing in parasite and liver sample but has been not recorded by databse. Identified proteins are presented in table 2, 3.

Bottom Line: To determine significant difference between two groups, two-sample t-test was performed.T-test analysis showed GST activity of parasite was lower than healthy liver tissue.SDS-PAGE showed GST protein bands with 24 kDa in parasite samples and25 kDa in liver tissues.

View Article: PubMed Central - PubMed

Affiliation: 1. Dept. of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Iran.

ABSTRACT

Background: The aim of this study was to detect the Glutathione S-Transferase(GST) enzyme activity of healthy / cystic liver as a diagnostic biomarker for hydatidosis. In order to compare with liver tissue, the level of the GSTs enzyme activity of parasite was also determined.

Methods: Parasites were collected from sheep liver tissue with hydatid cysts at a local abattoir and washed with PBS buffer. Collected parasites and liver tissues were sonicated or homogenized respectively. Extract solution samples were centrifuged and stored at - 20°C. GST enzyme activities were measured in the extract of parasite and liver tissue samples (healthy and infected livers). Protein amounts and protein bands were detected using Bradford and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) methods respectively. To determine significant difference between two groups, two-sample t-test was performed.

Results: GST specific activities of healthy / infected livers and parasites were estimated 304, 1297 and 146 U/ml/mg respectively. Significant higher GST specific activities in cystic liver than healthy liver was observed (P <0.05). T-test analysis showed GST activity of parasite was lower than healthy liver tissue. SDS-PAGE showed GST protein bands with 24 kDa in parasite samples and25 kDa in liver tissues.

Conclusion: GST activity incystic liver tissue could be concerned as a biomarker for hydatid cyst diagnosis with other hydatid disease parameters.

No MeSH data available.


Related in: MedlinePlus