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Molecular codes defining rostrocaudal domains in the embryonic mouse hypothalamus.

Ferran JL, Puelles L, Rubenstein JL - Front Neuroanat (2015)

Bottom Line: A number of markers were expressed in Thy (Fgf15, Gsc, Nkx6.2, Otx1, Zic1/5), but were absent in PHy, while other genes showed the converse pattern (Erbb4, Irx1/3/5, Lmo4, Mfap4, Plagl1, Pmch).We also identified markers that selectively label the ATD (Fgf8/10/18, Otx2, Pomc, Rax, Six6).On the whole, these data help to explain why, irrespective of the observed continuity of all dorsoventral molecular hypothalamic subdivisions across PHy and THy, different nuclear structures originate within each of these two domains, and also why singular structures arise at the ATD, e.g., the suprachiasmatic nuclei, the arcuate nucleus, the median eminence and the neurohypophysis.

View Article: PubMed Central - PubMed

Affiliation: Department of Human Anatomy and Psychobiology, School Medicine, University of Murcia and IMIB (Instituto Murciano de Investigación Biosanitaria) Murcia, Spain.

ABSTRACT
The prosomeric model proposes that the hypothalamus is a rostral forebrain entity, placed ventral to the telencephalon and rostral to the diencephalon. Gene expression markers differentially label molecularly distinct dorsoventral progenitor domains, which represent continuous longitudinal bands across the hypothalamic alar and basal regions. There is also circumstantial support for a rostrocaudal subdivision of the hypothalamus into transverse peduncular (caudal) and terminal (rostral) territories (PHy, THy). In addition, there is evidence for a specialized acroterminal domain at the rostral midline of the terminal hypothalamus (ATD). The PHy and THy transverse structural units are presently held to form part of two hypothalamo-telencephalic prosomeres (hp1 and hp2, respectively), which end dorsally at the telencephalic septocommissural roof. PHy and THy have distinct adult nuclei, at all dorsoventral levels. Here we report the results of data mining from the Allen Developing Mouse Brain Atlas database, looking for genes expressed differentially in the PHy, Thy, and ATD regions of the hypothalamus at several developmental stages. This search allowed us to identify additional molecular evidence supporting the postulated fundamental rostrocaudal bipartition of the mouse hypothalamus into the PHy and THy, and also corroborated molecularly the singularity of the ATD. A number of markers were expressed in Thy (Fgf15, Gsc, Nkx6.2, Otx1, Zic1/5), but were absent in PHy, while other genes showed the converse pattern (Erbb4, Irx1/3/5, Lmo4, Mfap4, Plagl1, Pmch). We also identified markers that selectively label the ATD (Fgf8/10/18, Otx2, Pomc, Rax, Six6). On the whole, these data help to explain why, irrespective of the observed continuity of all dorsoventral molecular hypothalamic subdivisions across PHy and THy, different nuclear structures originate within each of these two domains, and also why singular structures arise at the ATD, e.g., the suprachiasmatic nuclei, the arcuate nucleus, the median eminence and the neurohypophysis.

No MeSH data available.


Related in: MedlinePlus

Schematic maps of characteristic genoarchitectonic patterns in the hypothalamus, illustrating studied patterns selective for the terminal (THy) or peduncular (PHy) hypothalamic domains. Compare subdivisions with Figure 1. (A)Nkx6.2 was detected in the TuD domain of THy. (B)Otx1 expression was observed in the PM, M, and FP areas across ATHy and THy. (C)Rgs4 was detected at the basal TuI, TuV, and M areas from THy, as well as throughout the Pa area from PHy. (D)Plagl1 was detected at the basal RTuI, RTuV, PRM, and RM from PHy. (E)Lmo4 and Mfap4 were expressed selectively at the CPa and VPa paraventricular subdivisions within alar PHy. (F)Meis2 signal (red tag) was detected at the small PSPa domain; FoxB2 (red tag) was observed in a previously unknown dorsal subdivision of M domain (THy). (G)Pmch signal was detected only at the RTuD domain from basal PHy. (H)Erbb4, Irx1, Irx3, and Irx5 were detected at the RM and PHy FP.
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Figure 7: Schematic maps of characteristic genoarchitectonic patterns in the hypothalamus, illustrating studied patterns selective for the terminal (THy) or peduncular (PHy) hypothalamic domains. Compare subdivisions with Figure 1. (A)Nkx6.2 was detected in the TuD domain of THy. (B)Otx1 expression was observed in the PM, M, and FP areas across ATHy and THy. (C)Rgs4 was detected at the basal TuI, TuV, and M areas from THy, as well as throughout the Pa area from PHy. (D)Plagl1 was detected at the basal RTuI, RTuV, PRM, and RM from PHy. (E)Lmo4 and Mfap4 were expressed selectively at the CPa and VPa paraventricular subdivisions within alar PHy. (F)Meis2 signal (red tag) was detected at the small PSPa domain; FoxB2 (red tag) was observed in a previously unknown dorsal subdivision of M domain (THy). (G)Pmch signal was detected only at the RTuD domain from basal PHy. (H)Erbb4, Irx1, Irx3, and Irx5 were detected at the RM and PHy FP.

Mentions: Gsc appears expressed selectively at E15.5 within the peduncular portion of the subparaventricular area, apart separate expression in the terminal paraventricular area is observed (Figures 3Q, 6G). Another marker distinguishing this PHy subparaventricular domain is Meis2. At E13.5 its expression seems to extend partly into the THy (Figure 4A), but at E18.5 the signal is limited strictly to PHy (Figures 4B, 7F). Moreover, Vax1 shows at E15.5 differential subparaventricular labeling, with weaker and more disperse signal within the THy (suprachiasmatic and anterior hypothalamic nuclei), including some ventrally migrated cells in the underlying tuberal area, and more compact signal within the PHy component (Figure 4C).


Molecular codes defining rostrocaudal domains in the embryonic mouse hypothalamus.

Ferran JL, Puelles L, Rubenstein JL - Front Neuroanat (2015)

Schematic maps of characteristic genoarchitectonic patterns in the hypothalamus, illustrating studied patterns selective for the terminal (THy) or peduncular (PHy) hypothalamic domains. Compare subdivisions with Figure 1. (A)Nkx6.2 was detected in the TuD domain of THy. (B)Otx1 expression was observed in the PM, M, and FP areas across ATHy and THy. (C)Rgs4 was detected at the basal TuI, TuV, and M areas from THy, as well as throughout the Pa area from PHy. (D)Plagl1 was detected at the basal RTuI, RTuV, PRM, and RM from PHy. (E)Lmo4 and Mfap4 were expressed selectively at the CPa and VPa paraventricular subdivisions within alar PHy. (F)Meis2 signal (red tag) was detected at the small PSPa domain; FoxB2 (red tag) was observed in a previously unknown dorsal subdivision of M domain (THy). (G)Pmch signal was detected only at the RTuD domain from basal PHy. (H)Erbb4, Irx1, Irx3, and Irx5 were detected at the RM and PHy FP.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400913&req=5

Figure 7: Schematic maps of characteristic genoarchitectonic patterns in the hypothalamus, illustrating studied patterns selective for the terminal (THy) or peduncular (PHy) hypothalamic domains. Compare subdivisions with Figure 1. (A)Nkx6.2 was detected in the TuD domain of THy. (B)Otx1 expression was observed in the PM, M, and FP areas across ATHy and THy. (C)Rgs4 was detected at the basal TuI, TuV, and M areas from THy, as well as throughout the Pa area from PHy. (D)Plagl1 was detected at the basal RTuI, RTuV, PRM, and RM from PHy. (E)Lmo4 and Mfap4 were expressed selectively at the CPa and VPa paraventricular subdivisions within alar PHy. (F)Meis2 signal (red tag) was detected at the small PSPa domain; FoxB2 (red tag) was observed in a previously unknown dorsal subdivision of M domain (THy). (G)Pmch signal was detected only at the RTuD domain from basal PHy. (H)Erbb4, Irx1, Irx3, and Irx5 were detected at the RM and PHy FP.
Mentions: Gsc appears expressed selectively at E15.5 within the peduncular portion of the subparaventricular area, apart separate expression in the terminal paraventricular area is observed (Figures 3Q, 6G). Another marker distinguishing this PHy subparaventricular domain is Meis2. At E13.5 its expression seems to extend partly into the THy (Figure 4A), but at E18.5 the signal is limited strictly to PHy (Figures 4B, 7F). Moreover, Vax1 shows at E15.5 differential subparaventricular labeling, with weaker and more disperse signal within the THy (suprachiasmatic and anterior hypothalamic nuclei), including some ventrally migrated cells in the underlying tuberal area, and more compact signal within the PHy component (Figure 4C).

Bottom Line: A number of markers were expressed in Thy (Fgf15, Gsc, Nkx6.2, Otx1, Zic1/5), but were absent in PHy, while other genes showed the converse pattern (Erbb4, Irx1/3/5, Lmo4, Mfap4, Plagl1, Pmch).We also identified markers that selectively label the ATD (Fgf8/10/18, Otx2, Pomc, Rax, Six6).On the whole, these data help to explain why, irrespective of the observed continuity of all dorsoventral molecular hypothalamic subdivisions across PHy and THy, different nuclear structures originate within each of these two domains, and also why singular structures arise at the ATD, e.g., the suprachiasmatic nuclei, the arcuate nucleus, the median eminence and the neurohypophysis.

View Article: PubMed Central - PubMed

Affiliation: Department of Human Anatomy and Psychobiology, School Medicine, University of Murcia and IMIB (Instituto Murciano de Investigación Biosanitaria) Murcia, Spain.

ABSTRACT
The prosomeric model proposes that the hypothalamus is a rostral forebrain entity, placed ventral to the telencephalon and rostral to the diencephalon. Gene expression markers differentially label molecularly distinct dorsoventral progenitor domains, which represent continuous longitudinal bands across the hypothalamic alar and basal regions. There is also circumstantial support for a rostrocaudal subdivision of the hypothalamus into transverse peduncular (caudal) and terminal (rostral) territories (PHy, THy). In addition, there is evidence for a specialized acroterminal domain at the rostral midline of the terminal hypothalamus (ATD). The PHy and THy transverse structural units are presently held to form part of two hypothalamo-telencephalic prosomeres (hp1 and hp2, respectively), which end dorsally at the telencephalic septocommissural roof. PHy and THy have distinct adult nuclei, at all dorsoventral levels. Here we report the results of data mining from the Allen Developing Mouse Brain Atlas database, looking for genes expressed differentially in the PHy, Thy, and ATD regions of the hypothalamus at several developmental stages. This search allowed us to identify additional molecular evidence supporting the postulated fundamental rostrocaudal bipartition of the mouse hypothalamus into the PHy and THy, and also corroborated molecularly the singularity of the ATD. A number of markers were expressed in Thy (Fgf15, Gsc, Nkx6.2, Otx1, Zic1/5), but were absent in PHy, while other genes showed the converse pattern (Erbb4, Irx1/3/5, Lmo4, Mfap4, Plagl1, Pmch). We also identified markers that selectively label the ATD (Fgf8/10/18, Otx2, Pomc, Rax, Six6). On the whole, these data help to explain why, irrespective of the observed continuity of all dorsoventral molecular hypothalamic subdivisions across PHy and THy, different nuclear structures originate within each of these two domains, and also why singular structures arise at the ATD, e.g., the suprachiasmatic nuclei, the arcuate nucleus, the median eminence and the neurohypophysis.

No MeSH data available.


Related in: MedlinePlus