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Molecular codes defining rostrocaudal domains in the embryonic mouse hypothalamus.

Ferran JL, Puelles L, Rubenstein JL - Front Neuroanat (2015)

Bottom Line: A number of markers were expressed in Thy (Fgf15, Gsc, Nkx6.2, Otx1, Zic1/5), but were absent in PHy, while other genes showed the converse pattern (Erbb4, Irx1/3/5, Lmo4, Mfap4, Plagl1, Pmch).We also identified markers that selectively label the ATD (Fgf8/10/18, Otx2, Pomc, Rax, Six6).On the whole, these data help to explain why, irrespective of the observed continuity of all dorsoventral molecular hypothalamic subdivisions across PHy and THy, different nuclear structures originate within each of these two domains, and also why singular structures arise at the ATD, e.g., the suprachiasmatic nuclei, the arcuate nucleus, the median eminence and the neurohypophysis.

View Article: PubMed Central - PubMed

Affiliation: Department of Human Anatomy and Psychobiology, School Medicine, University of Murcia and IMIB (Instituto Murciano de Investigación Biosanitaria) Murcia, Spain.

ABSTRACT
The prosomeric model proposes that the hypothalamus is a rostral forebrain entity, placed ventral to the telencephalon and rostral to the diencephalon. Gene expression markers differentially label molecularly distinct dorsoventral progenitor domains, which represent continuous longitudinal bands across the hypothalamic alar and basal regions. There is also circumstantial support for a rostrocaudal subdivision of the hypothalamus into transverse peduncular (caudal) and terminal (rostral) territories (PHy, THy). In addition, there is evidence for a specialized acroterminal domain at the rostral midline of the terminal hypothalamus (ATD). The PHy and THy transverse structural units are presently held to form part of two hypothalamo-telencephalic prosomeres (hp1 and hp2, respectively), which end dorsally at the telencephalic septocommissural roof. PHy and THy have distinct adult nuclei, at all dorsoventral levels. Here we report the results of data mining from the Allen Developing Mouse Brain Atlas database, looking for genes expressed differentially in the PHy, Thy, and ATD regions of the hypothalamus at several developmental stages. This search allowed us to identify additional molecular evidence supporting the postulated fundamental rostrocaudal bipartition of the mouse hypothalamus into the PHy and THy, and also corroborated molecularly the singularity of the ATD. A number of markers were expressed in Thy (Fgf15, Gsc, Nkx6.2, Otx1, Zic1/5), but were absent in PHy, while other genes showed the converse pattern (Erbb4, Irx1/3/5, Lmo4, Mfap4, Plagl1, Pmch). We also identified markers that selectively label the ATD (Fgf8/10/18, Otx2, Pomc, Rax, Six6). On the whole, these data help to explain why, irrespective of the observed continuity of all dorsoventral molecular hypothalamic subdivisions across PHy and THy, different nuclear structures originate within each of these two domains, and also why singular structures arise at the ATD, e.g., the suprachiasmatic nuclei, the arcuate nucleus, the median eminence and the neurohypophysis.

No MeSH data available.


Related in: MedlinePlus

Sagittal, parasagittal (A–H,J–L,O,P,T) and transversal (I,M,N,Q–S) sections through the mouse secondary prosencephalon and diencephalon at E11.5, E13.5, E15.5, and E18.5, showing relevant examples of the hypothalamic genes expressed selectively at the terminal (THy), peduncular (PHy) or acroterminal (ATD) territories: Meis2 (A,B), Vax1 (C), Pmch (D,E), Nkx6.2 (F), Lef1 (G), Nr5a1 (H–J), Satb2 (K–O), Tcf7l2 (P–S), and Rprm (T). All images were downloaded from the Allen Developing Mouse Brain Atlas (http://developingmouse.brain-map.org/). For abbreviations, see the list. Red dotted line: THy/PHy boundary. Blue dotted line: PHy/p3 boundary.
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Figure 4: Sagittal, parasagittal (A–H,J–L,O,P,T) and transversal (I,M,N,Q–S) sections through the mouse secondary prosencephalon and diencephalon at E11.5, E13.5, E15.5, and E18.5, showing relevant examples of the hypothalamic genes expressed selectively at the terminal (THy), peduncular (PHy) or acroterminal (ATD) territories: Meis2 (A,B), Vax1 (C), Pmch (D,E), Nkx6.2 (F), Lef1 (G), Nr5a1 (H–J), Satb2 (K–O), Tcf7l2 (P–S), and Rprm (T). All images were downloaded from the Allen Developing Mouse Brain Atlas (http://developingmouse.brain-map.org/). For abbreviations, see the list. Red dotted line: THy/PHy boundary. Blue dotted line: PHy/p3 boundary.

Mentions: We found expression of Fgf15 restricted at E11.5 to the terminal alar hypothalamus, though leaving the acroterminal optic stalk negative (Figure 3M). Gsc expression was observed selectively in the terminal paraventricular zone at E13.5 and E15.5 (Figure 3Q). The terminal pattern of Nkx6.2 was described above (Figure 4F).


Molecular codes defining rostrocaudal domains in the embryonic mouse hypothalamus.

Ferran JL, Puelles L, Rubenstein JL - Front Neuroanat (2015)

Sagittal, parasagittal (A–H,J–L,O,P,T) and transversal (I,M,N,Q–S) sections through the mouse secondary prosencephalon and diencephalon at E11.5, E13.5, E15.5, and E18.5, showing relevant examples of the hypothalamic genes expressed selectively at the terminal (THy), peduncular (PHy) or acroterminal (ATD) territories: Meis2 (A,B), Vax1 (C), Pmch (D,E), Nkx6.2 (F), Lef1 (G), Nr5a1 (H–J), Satb2 (K–O), Tcf7l2 (P–S), and Rprm (T). All images were downloaded from the Allen Developing Mouse Brain Atlas (http://developingmouse.brain-map.org/). For abbreviations, see the list. Red dotted line: THy/PHy boundary. Blue dotted line: PHy/p3 boundary.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400913&req=5

Figure 4: Sagittal, parasagittal (A–H,J–L,O,P,T) and transversal (I,M,N,Q–S) sections through the mouse secondary prosencephalon and diencephalon at E11.5, E13.5, E15.5, and E18.5, showing relevant examples of the hypothalamic genes expressed selectively at the terminal (THy), peduncular (PHy) or acroterminal (ATD) territories: Meis2 (A,B), Vax1 (C), Pmch (D,E), Nkx6.2 (F), Lef1 (G), Nr5a1 (H–J), Satb2 (K–O), Tcf7l2 (P–S), and Rprm (T). All images were downloaded from the Allen Developing Mouse Brain Atlas (http://developingmouse.brain-map.org/). For abbreviations, see the list. Red dotted line: THy/PHy boundary. Blue dotted line: PHy/p3 boundary.
Mentions: We found expression of Fgf15 restricted at E11.5 to the terminal alar hypothalamus, though leaving the acroterminal optic stalk negative (Figure 3M). Gsc expression was observed selectively in the terminal paraventricular zone at E13.5 and E15.5 (Figure 3Q). The terminal pattern of Nkx6.2 was described above (Figure 4F).

Bottom Line: A number of markers were expressed in Thy (Fgf15, Gsc, Nkx6.2, Otx1, Zic1/5), but were absent in PHy, while other genes showed the converse pattern (Erbb4, Irx1/3/5, Lmo4, Mfap4, Plagl1, Pmch).We also identified markers that selectively label the ATD (Fgf8/10/18, Otx2, Pomc, Rax, Six6).On the whole, these data help to explain why, irrespective of the observed continuity of all dorsoventral molecular hypothalamic subdivisions across PHy and THy, different nuclear structures originate within each of these two domains, and also why singular structures arise at the ATD, e.g., the suprachiasmatic nuclei, the arcuate nucleus, the median eminence and the neurohypophysis.

View Article: PubMed Central - PubMed

Affiliation: Department of Human Anatomy and Psychobiology, School Medicine, University of Murcia and IMIB (Instituto Murciano de Investigación Biosanitaria) Murcia, Spain.

ABSTRACT
The prosomeric model proposes that the hypothalamus is a rostral forebrain entity, placed ventral to the telencephalon and rostral to the diencephalon. Gene expression markers differentially label molecularly distinct dorsoventral progenitor domains, which represent continuous longitudinal bands across the hypothalamic alar and basal regions. There is also circumstantial support for a rostrocaudal subdivision of the hypothalamus into transverse peduncular (caudal) and terminal (rostral) territories (PHy, THy). In addition, there is evidence for a specialized acroterminal domain at the rostral midline of the terminal hypothalamus (ATD). The PHy and THy transverse structural units are presently held to form part of two hypothalamo-telencephalic prosomeres (hp1 and hp2, respectively), which end dorsally at the telencephalic septocommissural roof. PHy and THy have distinct adult nuclei, at all dorsoventral levels. Here we report the results of data mining from the Allen Developing Mouse Brain Atlas database, looking for genes expressed differentially in the PHy, Thy, and ATD regions of the hypothalamus at several developmental stages. This search allowed us to identify additional molecular evidence supporting the postulated fundamental rostrocaudal bipartition of the mouse hypothalamus into the PHy and THy, and also corroborated molecularly the singularity of the ATD. A number of markers were expressed in Thy (Fgf15, Gsc, Nkx6.2, Otx1, Zic1/5), but were absent in PHy, while other genes showed the converse pattern (Erbb4, Irx1/3/5, Lmo4, Mfap4, Plagl1, Pmch). We also identified markers that selectively label the ATD (Fgf8/10/18, Otx2, Pomc, Rax, Six6). On the whole, these data help to explain why, irrespective of the observed continuity of all dorsoventral molecular hypothalamic subdivisions across PHy and THy, different nuclear structures originate within each of these two domains, and also why singular structures arise at the ATD, e.g., the suprachiasmatic nuclei, the arcuate nucleus, the median eminence and the neurohypophysis.

No MeSH data available.


Related in: MedlinePlus