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Molecular codes defining rostrocaudal domains in the embryonic mouse hypothalamus.

Ferran JL, Puelles L, Rubenstein JL - Front Neuroanat (2015)

Bottom Line: A number of markers were expressed in Thy (Fgf15, Gsc, Nkx6.2, Otx1, Zic1/5), but were absent in PHy, while other genes showed the converse pattern (Erbb4, Irx1/3/5, Lmo4, Mfap4, Plagl1, Pmch).We also identified markers that selectively label the ATD (Fgf8/10/18, Otx2, Pomc, Rax, Six6).On the whole, these data help to explain why, irrespective of the observed continuity of all dorsoventral molecular hypothalamic subdivisions across PHy and THy, different nuclear structures originate within each of these two domains, and also why singular structures arise at the ATD, e.g., the suprachiasmatic nuclei, the arcuate nucleus, the median eminence and the neurohypophysis.

View Article: PubMed Central - PubMed

Affiliation: Department of Human Anatomy and Psychobiology, School Medicine, University of Murcia and IMIB (Instituto Murciano de Investigación Biosanitaria) Murcia, Spain.

ABSTRACT
The prosomeric model proposes that the hypothalamus is a rostral forebrain entity, placed ventral to the telencephalon and rostral to the diencephalon. Gene expression markers differentially label molecularly distinct dorsoventral progenitor domains, which represent continuous longitudinal bands across the hypothalamic alar and basal regions. There is also circumstantial support for a rostrocaudal subdivision of the hypothalamus into transverse peduncular (caudal) and terminal (rostral) territories (PHy, THy). In addition, there is evidence for a specialized acroterminal domain at the rostral midline of the terminal hypothalamus (ATD). The PHy and THy transverse structural units are presently held to form part of two hypothalamo-telencephalic prosomeres (hp1 and hp2, respectively), which end dorsally at the telencephalic septocommissural roof. PHy and THy have distinct adult nuclei, at all dorsoventral levels. Here we report the results of data mining from the Allen Developing Mouse Brain Atlas database, looking for genes expressed differentially in the PHy, Thy, and ATD regions of the hypothalamus at several developmental stages. This search allowed us to identify additional molecular evidence supporting the postulated fundamental rostrocaudal bipartition of the mouse hypothalamus into the PHy and THy, and also corroborated molecularly the singularity of the ATD. A number of markers were expressed in Thy (Fgf15, Gsc, Nkx6.2, Otx1, Zic1/5), but were absent in PHy, while other genes showed the converse pattern (Erbb4, Irx1/3/5, Lmo4, Mfap4, Plagl1, Pmch). We also identified markers that selectively label the ATD (Fgf8/10/18, Otx2, Pomc, Rax, Six6). On the whole, these data help to explain why, irrespective of the observed continuity of all dorsoventral molecular hypothalamic subdivisions across PHy and THy, different nuclear structures originate within each of these two domains, and also why singular structures arise at the ATD, e.g., the suprachiasmatic nuclei, the arcuate nucleus, the median eminence and the neurohypophysis.

No MeSH data available.


Related in: MedlinePlus

Sagittal and parasagittal (A–R) sections through the mouse secondary prosencephalon and diencephalon at E11.5, E13.5, E15.5, and one P4 image, showing relevant examples of hypothalamic genes expressed selectively at the terminal (THy) and peduncular (PHy) territories: Zic5 (A–D), Otp (E), Zic1 (F), Ascl1 (G), Tbr1 (H), Rgs4 (I–L), Fgf15 (M), Lmo4 (N,O), Mfap4 (P), Gsc (Q) and Plagl1 (R). All images were downloaded from the Allen Developing Mouse Brain Atlas (http://developingmouse.brain-map.org/). For abbreviations, see the list. Red dotted line: THy/PHy boundary. Blue dotted line: PHy/p3 boundary.
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Figure 3: Sagittal and parasagittal (A–R) sections through the mouse secondary prosencephalon and diencephalon at E11.5, E13.5, E15.5, and one P4 image, showing relevant examples of hypothalamic genes expressed selectively at the terminal (THy) and peduncular (PHy) territories: Zic5 (A–D), Otp (E), Zic1 (F), Ascl1 (G), Tbr1 (H), Rgs4 (I–L), Fgf15 (M), Lmo4 (N,O), Mfap4 (P), Gsc (Q) and Plagl1 (R). All images were downloaded from the Allen Developing Mouse Brain Atlas (http://developingmouse.brain-map.org/). For abbreviations, see the list. Red dotted line: THy/PHy boundary. Blue dotted line: PHy/p3 boundary.

Mentions: Zic5, belonging to the Zic gene family related to the specification of alar neural regions, shows an interesting dynamic expression pattern. At E13.5 its signal appears restricted to the ventricular zone of the alar paraventricular area across both THy and PHy (extending into the eye stalk, which is an ATD component). However, this expression is distinctly weaker at PHy than at THy (Figures 3A,B). At E15,5, the paraventricular mantle zone shows strong expression (some cells also disperse into the subjacent subparaventricular area), but only at the THy moiety (Figures 3C, 6F). This situation clearly remains unchanged at P4 (Figure 3D; note the blank main paraventricular nucleus and the negative suprachiasmatic nucleus. For comparison we inserted the pattern of Otp, which shows an equally dense labeled mantle in THy and PHy (Figure 3E), and the pattern of Tbr1 at P4, which emphasizes the PHy derivative of the paraventricular area (Figure 3H, to compare with Figure 3D).


Molecular codes defining rostrocaudal domains in the embryonic mouse hypothalamus.

Ferran JL, Puelles L, Rubenstein JL - Front Neuroanat (2015)

Sagittal and parasagittal (A–R) sections through the mouse secondary prosencephalon and diencephalon at E11.5, E13.5, E15.5, and one P4 image, showing relevant examples of hypothalamic genes expressed selectively at the terminal (THy) and peduncular (PHy) territories: Zic5 (A–D), Otp (E), Zic1 (F), Ascl1 (G), Tbr1 (H), Rgs4 (I–L), Fgf15 (M), Lmo4 (N,O), Mfap4 (P), Gsc (Q) and Plagl1 (R). All images were downloaded from the Allen Developing Mouse Brain Atlas (http://developingmouse.brain-map.org/). For abbreviations, see the list. Red dotted line: THy/PHy boundary. Blue dotted line: PHy/p3 boundary.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400913&req=5

Figure 3: Sagittal and parasagittal (A–R) sections through the mouse secondary prosencephalon and diencephalon at E11.5, E13.5, E15.5, and one P4 image, showing relevant examples of hypothalamic genes expressed selectively at the terminal (THy) and peduncular (PHy) territories: Zic5 (A–D), Otp (E), Zic1 (F), Ascl1 (G), Tbr1 (H), Rgs4 (I–L), Fgf15 (M), Lmo4 (N,O), Mfap4 (P), Gsc (Q) and Plagl1 (R). All images were downloaded from the Allen Developing Mouse Brain Atlas (http://developingmouse.brain-map.org/). For abbreviations, see the list. Red dotted line: THy/PHy boundary. Blue dotted line: PHy/p3 boundary.
Mentions: Zic5, belonging to the Zic gene family related to the specification of alar neural regions, shows an interesting dynamic expression pattern. At E13.5 its signal appears restricted to the ventricular zone of the alar paraventricular area across both THy and PHy (extending into the eye stalk, which is an ATD component). However, this expression is distinctly weaker at PHy than at THy (Figures 3A,B). At E15,5, the paraventricular mantle zone shows strong expression (some cells also disperse into the subjacent subparaventricular area), but only at the THy moiety (Figures 3C, 6F). This situation clearly remains unchanged at P4 (Figure 3D; note the blank main paraventricular nucleus and the negative suprachiasmatic nucleus. For comparison we inserted the pattern of Otp, which shows an equally dense labeled mantle in THy and PHy (Figure 3E), and the pattern of Tbr1 at P4, which emphasizes the PHy derivative of the paraventricular area (Figure 3H, to compare with Figure 3D).

Bottom Line: A number of markers were expressed in Thy (Fgf15, Gsc, Nkx6.2, Otx1, Zic1/5), but were absent in PHy, while other genes showed the converse pattern (Erbb4, Irx1/3/5, Lmo4, Mfap4, Plagl1, Pmch).We also identified markers that selectively label the ATD (Fgf8/10/18, Otx2, Pomc, Rax, Six6).On the whole, these data help to explain why, irrespective of the observed continuity of all dorsoventral molecular hypothalamic subdivisions across PHy and THy, different nuclear structures originate within each of these two domains, and also why singular structures arise at the ATD, e.g., the suprachiasmatic nuclei, the arcuate nucleus, the median eminence and the neurohypophysis.

View Article: PubMed Central - PubMed

Affiliation: Department of Human Anatomy and Psychobiology, School Medicine, University of Murcia and IMIB (Instituto Murciano de Investigación Biosanitaria) Murcia, Spain.

ABSTRACT
The prosomeric model proposes that the hypothalamus is a rostral forebrain entity, placed ventral to the telencephalon and rostral to the diencephalon. Gene expression markers differentially label molecularly distinct dorsoventral progenitor domains, which represent continuous longitudinal bands across the hypothalamic alar and basal regions. There is also circumstantial support for a rostrocaudal subdivision of the hypothalamus into transverse peduncular (caudal) and terminal (rostral) territories (PHy, THy). In addition, there is evidence for a specialized acroterminal domain at the rostral midline of the terminal hypothalamus (ATD). The PHy and THy transverse structural units are presently held to form part of two hypothalamo-telencephalic prosomeres (hp1 and hp2, respectively), which end dorsally at the telencephalic septocommissural roof. PHy and THy have distinct adult nuclei, at all dorsoventral levels. Here we report the results of data mining from the Allen Developing Mouse Brain Atlas database, looking for genes expressed differentially in the PHy, Thy, and ATD regions of the hypothalamus at several developmental stages. This search allowed us to identify additional molecular evidence supporting the postulated fundamental rostrocaudal bipartition of the mouse hypothalamus into the PHy and THy, and also corroborated molecularly the singularity of the ATD. A number of markers were expressed in Thy (Fgf15, Gsc, Nkx6.2, Otx1, Zic1/5), but were absent in PHy, while other genes showed the converse pattern (Erbb4, Irx1/3/5, Lmo4, Mfap4, Plagl1, Pmch). We also identified markers that selectively label the ATD (Fgf8/10/18, Otx2, Pomc, Rax, Six6). On the whole, these data help to explain why, irrespective of the observed continuity of all dorsoventral molecular hypothalamic subdivisions across PHy and THy, different nuclear structures originate within each of these two domains, and also why singular structures arise at the ATD, e.g., the suprachiasmatic nuclei, the arcuate nucleus, the median eminence and the neurohypophysis.

No MeSH data available.


Related in: MedlinePlus