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Antimicrobial activities of chicken β-defensin (4 and 10) peptides against pathogenic bacteria and fungi.

Yacoub HA, Elazzazy AM, Abuzinadah OA, Al-Hejin AM, Mahmoud MM, Harakeh SM - Front Cell Infect Microbiol (2015)

Bottom Line: Host Defense Peptides (HDPs) are small cationic peptides found in several organisms.Based on blood hemolysis studies, a little hemolytic effect was showed in the case of both peptides even when applied at high concentrations.In conclusion, future work and research should be tailored to a better understanding of the mechanisms of action of those peptides and their potential use in the pharmaceutical industry to help reduce the incidence and impact of infectious agent and be marketed as a naturally occurring antibiotic.

View Article: PubMed Central - PubMed

Affiliation: Biological Sciences Department, Faculty of Sciences, King Abdulaziz University Jeddah, Saudi Arabia ; Genetic Engineering and Biotechnology Division, Cell Biology Department, National Research Centre Gizza, Egypt.

ABSTRACT
Host Defense Peptides (HDPs) are small cationic peptides found in several organisms. They play a vital role in innate immunity response and immunomodulatory stimulation. This investigation was designed to study the antimicrobial activities of β-defensin peptide-4 (sAvBD-4) and 10 (sAvBD-4) derived from chickens against pathogenic organisms including bacteria and fungi. Ten bacterial strains and three fungal species were used in investigation. The results showed that the sAvBD-10 displayed a higher bactericidal potency against all the tested bacterial strains than that of sAvBD-4. The exhibited bactericidal activity was significant against almost the different bacterial strains at different peptide concentrations except for that of Pseudomonas aeruginosa (P. aeruginosa) and Streptococcus bovis (Str. bovis) strains where a moderate effect was noted. Both peptides were effective in the inactivation of fungal species tested yielding a killing rate of up to 95%. The results revealed that the synthetic peptides were resistant to salt at a concentration of 50 mM NaCl. However, they lost antimicrobial potency when applied in the presence of high salt concentrations. Based on blood hemolysis studies, a little hemolytic effect was showed in the case of both peptides even when applied at high concentrations. The data obtained from this study indicated that synthetic avian peptides exhibit strong antibacterial and antifungal activity. In conclusion, future work and research should be tailored to a better understanding of the mechanisms of action of those peptides and their potential use in the pharmaceutical industry to help reduce the incidence and impact of infectious agent and be marketed as a naturally occurring antibiotic.

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Related in: MedlinePlus

Hemolytic activities of synthetic chicken β-defensin-4 and 10-derived peptides (sAvBD). All assays were performed in three independent experiments and each point is the mean ± SE.
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Figure 4: Hemolytic activities of synthetic chicken β-defensin-4 and 10-derived peptides (sAvBD). All assays were performed in three independent experiments and each point is the mean ± SE.

Mentions: The hemolytic activities of the two peptides were evaluated using freshly isolated chicken erythrocytes. The hemolysis was done using spectrophotometric measurements at a wavelength of 405 nm (Figure 4). The results showed that the peptides did not produce significant hemolytic effects.


Antimicrobial activities of chicken β-defensin (4 and 10) peptides against pathogenic bacteria and fungi.

Yacoub HA, Elazzazy AM, Abuzinadah OA, Al-Hejin AM, Mahmoud MM, Harakeh SM - Front Cell Infect Microbiol (2015)

Hemolytic activities of synthetic chicken β-defensin-4 and 10-derived peptides (sAvBD). All assays were performed in three independent experiments and each point is the mean ± SE.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400880&req=5

Figure 4: Hemolytic activities of synthetic chicken β-defensin-4 and 10-derived peptides (sAvBD). All assays were performed in three independent experiments and each point is the mean ± SE.
Mentions: The hemolytic activities of the two peptides were evaluated using freshly isolated chicken erythrocytes. The hemolysis was done using spectrophotometric measurements at a wavelength of 405 nm (Figure 4). The results showed that the peptides did not produce significant hemolytic effects.

Bottom Line: Host Defense Peptides (HDPs) are small cationic peptides found in several organisms.Based on blood hemolysis studies, a little hemolytic effect was showed in the case of both peptides even when applied at high concentrations.In conclusion, future work and research should be tailored to a better understanding of the mechanisms of action of those peptides and their potential use in the pharmaceutical industry to help reduce the incidence and impact of infectious agent and be marketed as a naturally occurring antibiotic.

View Article: PubMed Central - PubMed

Affiliation: Biological Sciences Department, Faculty of Sciences, King Abdulaziz University Jeddah, Saudi Arabia ; Genetic Engineering and Biotechnology Division, Cell Biology Department, National Research Centre Gizza, Egypt.

ABSTRACT
Host Defense Peptides (HDPs) are small cationic peptides found in several organisms. They play a vital role in innate immunity response and immunomodulatory stimulation. This investigation was designed to study the antimicrobial activities of β-defensin peptide-4 (sAvBD-4) and 10 (sAvBD-4) derived from chickens against pathogenic organisms including bacteria and fungi. Ten bacterial strains and three fungal species were used in investigation. The results showed that the sAvBD-10 displayed a higher bactericidal potency against all the tested bacterial strains than that of sAvBD-4. The exhibited bactericidal activity was significant against almost the different bacterial strains at different peptide concentrations except for that of Pseudomonas aeruginosa (P. aeruginosa) and Streptococcus bovis (Str. bovis) strains where a moderate effect was noted. Both peptides were effective in the inactivation of fungal species tested yielding a killing rate of up to 95%. The results revealed that the synthetic peptides were resistant to salt at a concentration of 50 mM NaCl. However, they lost antimicrobial potency when applied in the presence of high salt concentrations. Based on blood hemolysis studies, a little hemolytic effect was showed in the case of both peptides even when applied at high concentrations. The data obtained from this study indicated that synthetic avian peptides exhibit strong antibacterial and antifungal activity. In conclusion, future work and research should be tailored to a better understanding of the mechanisms of action of those peptides and their potential use in the pharmaceutical industry to help reduce the incidence and impact of infectious agent and be marketed as a naturally occurring antibiotic.

Show MeSH
Related in: MedlinePlus