Long-term expandable SOX9+ chondrogenic ectomesenchymal cells from human pluripotent stem cells.
Bottom Line: When "primed" with TGFβ, such cells efficiently formed translucent cartilage particles, which were completely mineralized in 12 weeks in immunocompromized mice.Ectomesenchyme is a source of many craniofacial bone and cartilage structures.The method we describe for obtaining a large quantity of human ectomesenchymal cells will help to model craniofacial disorders in vitro and potentially provide cells for the repair of craniofacial damage.
Affiliation: Institute of Molecular Medicine, The University of Texas Health Science Center at Houston Medical School, Houston, TX 77030, USA.Show MeSH
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Mentions: We performed genome-wide, comparative transcriptome analyses to elucidate the molecular basis of the effect of Nodal/Activin/TGFβ signaling-suppression on the maintenance of the proliferative and chondrogenic properties of the CD271+CD73+ ectomesenchymal cells. The CD271hiCD73− neural crest-like progeny of H9 hESCs were isolated and expanded under FGF2+SB431542 conditions for six to seven passages (FSB, Figure 6). In some cases, the culture condition was shifted to the FGF2 condition from p4 (FSB→F). As a control, the CD271hiCD73− cells were cultured from the outset under FGF2-conditions (F) for six passages.
Affiliation: Institute of Molecular Medicine, The University of Texas Health Science Center at Houston Medical School, Houston, TX 77030, USA.