Long-term expandable SOX9+ chondrogenic ectomesenchymal cells from human pluripotent stem cells.
Bottom Line: When "primed" with TGFβ, such cells efficiently formed translucent cartilage particles, which were completely mineralized in 12 weeks in immunocompromized mice.Ectomesenchyme is a source of many craniofacial bone and cartilage structures.The method we describe for obtaining a large quantity of human ectomesenchymal cells will help to model craniofacial disorders in vitro and potentially provide cells for the repair of craniofacial damage.
Affiliation: Institute of Molecular Medicine, The University of Texas Health Science Center at Houston Medical School, Houston, TX 77030, USA.Show MeSH
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Mentions: Since CD271+CD73+ ectomesenchymal cells maintained under FGF2+SB431542 for a long time might select a rare “permanent CD271+ chondrogenic cell” from the original neural crest cell population, late-passage (p10) cells were subjected to medium change to CDM with FGF2 alone. All cells started losing the CD271 expression in FGF2 and nearly 95% became CD271−CD73+ by passage 3 (Figure 5A). Those maintained in FGF2+SB431542 for four more passages were still CD271+CD73+, suggesting that maintenance of CD271 surface expression remained dependent on the presence of SB431542.
Affiliation: Institute of Molecular Medicine, The University of Texas Health Science Center at Houston Medical School, Houston, TX 77030, USA.