Direct reprogramming of human bone marrow stromal cells into functional renal cells using cell-free extracts.
Bottom Line: Transmission electron microscopy revealed the presence of brush border microvilli and tight intercellular contacts.RNA sequencing showed tubular epithelial transcript abundance and revealed the upregulation of components of the EGFR pathway.Thus, reprogrammed BMSCs are a promising cell resource for future cell therapy.
Affiliation: IRCCS-Istituto di Ricerche Farmacologiche "Mario Negri," Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, 24126 Bergamo, Italy. Electronic address: firstname.lastname@example.org.Show MeSH
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Mentions: Having observed the formation of nascent intercellular contacts, we next inquired whether CL17 cells in addition to morphological and cell-lineage markers had acquired physiological properties similar to the epithelial phenotype. Testing the electrical resistance of BMSC monolayers showed trivial values of 34 ± 10 Ohm/cm2. In contrast, monolayers of CL17 cells developed electrical resistance similar to that observed in HK2 cells (80 ± 10 versus 81 ± 12 Ohm/cm2) (Figure 4A), both statistically significantly different from the transepithelial resistance (TER) assessed in BMSCs.
Affiliation: IRCCS-Istituto di Ricerche Farmacologiche "Mario Negri," Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, 24126 Bergamo, Italy. Electronic address: email@example.com.