Direct reprogramming of human bone marrow stromal cells into functional renal cells using cell-free extracts.
Bottom Line: Transmission electron microscopy revealed the presence of brush border microvilli and tight intercellular contacts.RNA sequencing showed tubular epithelial transcript abundance and revealed the upregulation of components of the EGFR pathway.Thus, reprogrammed BMSCs are a promising cell resource for future cell therapy.
Affiliation: IRCCS-Istituto di Ricerche Farmacologiche "Mario Negri," Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, 24126 Bergamo, Italy. Electronic address: email@example.com.Show MeSH
Related in: MedlinePlus
Mentions: Human BMSCs were permeabilized with 400 ng/ml streptolysin O (SLO), a concentration that did not affect cell viability. Permeabilized BMSCs exposed to the extract of human proximal tubular epithelial (HK2) cells changed from their usual spindle-shape appearance (Figure 1A) to cobblestone islands within 13–15 days (Figures 1B–1D). During the subsequent 2 weeks, these islands expanded and the formation of “domes” and tubular-like structures (Figures 1E and 1F) similar to HK2 cells (Figure 1H) occurred. This morphological transition did not occur in BMSCs grown in epithelial-specific culture medium, even after 35 days (Figures 1I and 1J). The observed change in morphological appearance of BMSCs treated with epithelial cell extract suggested a phenotypic switch in the cells.
Affiliation: IRCCS-Istituto di Ricerche Farmacologiche "Mario Negri," Centro Anna Maria Astori, Science and Technology Park Kilometro Rosso, 24126 Bergamo, Italy. Electronic address: firstname.lastname@example.org.