The p53 isoform Δ133p53β promotes cancer stem cell potential.
Bottom Line: Here we report that Δ133p53β, a TP53 splice variant, enhanced cancer cell stemness in MCF-7 breast cancer cells, while its depletion reduced it.Like in MCF-7 cells, SOX2, OCT3/4, and NANOG expression were positively regulated by Δ133p53β in these cells.Our findings show that Δ133p53β supports CSC potential.
Affiliation: Centre National de la Recherche Scientifique, UMR 5237, Centre de Recherche en Biochimie Macromoléculaire, Université Montpellier, 1919 route de Mende, 34293 Montpellier Cedex 5, France.Show MeSH
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Mentions: Evaluation of mammosphere formation in D3H2LN and C3LND cells showed that C3LND cells formed two times more mammospheres (Figure 3A). Similarly, Δ133p53 isoform expression was 3-fold higher and OCT3/4, NANOG, and SOX2 levels were 2- to 3-fold higher in C3LND (Figures 3B and 3C). C-MYC expression was comparable in the two cells lines. We then asked whether pluripotency factor expression could be affected by changes in Δ133p53 expression. Overexpression of Δ133p53β in D3H2LN cells resulted in a significant increase of OCT3/4, NANOG, and SOX2 expression, whereas C-MYC level was not affected, consistent with data in MCF-7 cells (Figures 3D and 3E). Similar results were obtained in C3LND cells (Figure S3F). In complete agreement with observations in MCF-7, Δ133p53β overexpression in D3H2LN cells resulted in a significant increase of mammosphere formation (Figure 3F).
Affiliation: Centre National de la Recherche Scientifique, UMR 5237, Centre de Recherche en Biochimie Macromoléculaire, Université Montpellier, 1919 route de Mende, 34293 Montpellier Cedex 5, France.