Limits...
The p53 isoform Δ133p53β promotes cancer stem cell potential.

Arsic N, Gadea G, Lagerqvist EL, Busson M, Cahuzac N, Brock C, Hollande F, Gire V, Pannequin J, Roux P - Stem Cell Reports (2015)

Bottom Line: Here we report that Δ133p53β, a TP53 splice variant, enhanced cancer cell stemness in MCF-7 breast cancer cells, while its depletion reduced it.Like in MCF-7 cells, SOX2, OCT3/4, and NANOG expression were positively regulated by Δ133p53β in these cells.Our findings show that Δ133p53β supports CSC potential.

View Article: PubMed Central - PubMed

Affiliation: Centre National de la Recherche Scientifique, UMR 5237, Centre de Recherche en Biochimie Macromoléculaire, Université Montpellier, 1919 route de Mende, 34293 Montpellier Cedex 5, France.

Show MeSH

Related in: MedlinePlus

Selective Depletion of p53 Isoforms Affects the Sphere-Forming Ability of MCF-7 Cells(A) Schematic representation of p53 isoforms with the targets of the different shRNAs (Sh) used in this study. The calculated molecular weights of the different isoforms are indicated.(B) Mammosphere quantification in MCF-7 cells after transduction of Sh1, Sh2, Sh3, Sh4, and Sh5 (n = 3 independent experiments).(C–E) Western blot analysis of p53 isoform depletion in the corresponding cells.(F and G) The qRT-PCR quantification of the expression levels of C-MYC, SOX2, OCT3/4, and NANOG (F) as well as of Δ133p53 and p53 β isoforms (G) after transduction with Sh1 and Sh2 (n = 4 independent experiments).
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4400643&req=5

fig1: Selective Depletion of p53 Isoforms Affects the Sphere-Forming Ability of MCF-7 Cells(A) Schematic representation of p53 isoforms with the targets of the different shRNAs (Sh) used in this study. The calculated molecular weights of the different isoforms are indicated.(B) Mammosphere quantification in MCF-7 cells after transduction of Sh1, Sh2, Sh3, Sh4, and Sh5 (n = 3 independent experiments).(C–E) Western blot analysis of p53 isoform depletion in the corresponding cells.(F and G) The qRT-PCR quantification of the expression levels of C-MYC, SOX2, OCT3/4, and NANOG (F) as well as of Δ133p53 and p53 β isoforms (G) after transduction with Sh1 and Sh2 (n = 4 independent experiments).

Mentions: To study the role of the different p53 isoforms in CSC potential, we designed small hairpin RNAs (shRNAs) (Sh) that selectively silence specific groups of isoforms (Figure 1A; Table S1). Briefly, Sh1 knocks down all p53 isoforms, while Sh2 targets the long TAp53 (trans-activating) and Δ40p53 isoforms. Sh3 and Sh4 target the 5′ UTR of the Δ133 isoforms (α, β, and γ), and Sh5 and Sh6 respectively target the 3′ end of the β and α isoforms.


The p53 isoform Δ133p53β promotes cancer stem cell potential.

Arsic N, Gadea G, Lagerqvist EL, Busson M, Cahuzac N, Brock C, Hollande F, Gire V, Pannequin J, Roux P - Stem Cell Reports (2015)

Selective Depletion of p53 Isoforms Affects the Sphere-Forming Ability of MCF-7 Cells(A) Schematic representation of p53 isoforms with the targets of the different shRNAs (Sh) used in this study. The calculated molecular weights of the different isoforms are indicated.(B) Mammosphere quantification in MCF-7 cells after transduction of Sh1, Sh2, Sh3, Sh4, and Sh5 (n = 3 independent experiments).(C–E) Western blot analysis of p53 isoform depletion in the corresponding cells.(F and G) The qRT-PCR quantification of the expression levels of C-MYC, SOX2, OCT3/4, and NANOG (F) as well as of Δ133p53 and p53 β isoforms (G) after transduction with Sh1 and Sh2 (n = 4 independent experiments).
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400643&req=5

fig1: Selective Depletion of p53 Isoforms Affects the Sphere-Forming Ability of MCF-7 Cells(A) Schematic representation of p53 isoforms with the targets of the different shRNAs (Sh) used in this study. The calculated molecular weights of the different isoforms are indicated.(B) Mammosphere quantification in MCF-7 cells after transduction of Sh1, Sh2, Sh3, Sh4, and Sh5 (n = 3 independent experiments).(C–E) Western blot analysis of p53 isoform depletion in the corresponding cells.(F and G) The qRT-PCR quantification of the expression levels of C-MYC, SOX2, OCT3/4, and NANOG (F) as well as of Δ133p53 and p53 β isoforms (G) after transduction with Sh1 and Sh2 (n = 4 independent experiments).
Mentions: To study the role of the different p53 isoforms in CSC potential, we designed small hairpin RNAs (shRNAs) (Sh) that selectively silence specific groups of isoforms (Figure 1A; Table S1). Briefly, Sh1 knocks down all p53 isoforms, while Sh2 targets the long TAp53 (trans-activating) and Δ40p53 isoforms. Sh3 and Sh4 target the 5′ UTR of the Δ133 isoforms (α, β, and γ), and Sh5 and Sh6 respectively target the 3′ end of the β and α isoforms.

Bottom Line: Here we report that Δ133p53β, a TP53 splice variant, enhanced cancer cell stemness in MCF-7 breast cancer cells, while its depletion reduced it.Like in MCF-7 cells, SOX2, OCT3/4, and NANOG expression were positively regulated by Δ133p53β in these cells.Our findings show that Δ133p53β supports CSC potential.

View Article: PubMed Central - PubMed

Affiliation: Centre National de la Recherche Scientifique, UMR 5237, Centre de Recherche en Biochimie Macromoléculaire, Université Montpellier, 1919 route de Mende, 34293 Montpellier Cedex 5, France.

Show MeSH
Related in: MedlinePlus