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Models of breast morphogenesis based on localization of stem cells in the developing mammary lobule.

Honeth G, Schiavinotto T, Vaggi F, Marlow R, Kanno T, Shinomiya I, Lombardi S, Buchupalli B, Graham R, Gazinska P, Ramalingam V, Burchell J, Purushotham AD, Pinder SE, Csikasz-Nagy A, Dontu G - Stem Cell Reports (2015)

Bottom Line: However, the identity of these cells is a subject of controversy and their localization in the breast epithelium is not known.In this study, we utilized a novel approach to analyze the morphogenesis of mammary lobules, by combining one-dimensional theoretical models and computer-generated 3D fractals.An increased representation of stem cells was found in smaller, less developed lobules compared to larger, more mature lobules, with marked differences in the gland of iparous versus parous women and that of BRCA1/2 mutation carriers versus non-carriers.

View Article: PubMed Central - PubMed

Affiliation: Research Oncology, King's College London School of Medicine, London SE1 9RT, UK. Electronic address: gabriella.honeth@med.lu.se.

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Fractal Models of Mammary Lobules with Virtual Sections in Comparison to Real Tissue Sections(A) 3D trees generated by fractal modeling, representing mammary lobules with 6, 8, and 12 branching generations.(B) Virtual sections through the fractal trees shown in (A). The sectioning plane is indicated by the blue line in (A).(C) Real sections through normal breast lobules, corresponding to the fractal sections shown in (B) (H&E staining). Scale bar, 100 μm.See also Figure S5.
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fig4: Fractal Models of Mammary Lobules with Virtual Sections in Comparison to Real Tissue Sections(A) 3D trees generated by fractal modeling, representing mammary lobules with 6, 8, and 12 branching generations.(B) Virtual sections through the fractal trees shown in (A). The sectioning plane is indicated by the blue line in (A).(C) Real sections through normal breast lobules, corresponding to the fractal sections shown in (B) (H&E staining). Scale bar, 100 μm.See also Figure S5.

Mentions: To better elucidate the localization of stem cell markers in the 3D architecture of the mammary lobule, we utilized computer-generated 3D fractal models and compared virtual sections with equivalent immunostained tissue sections (Figure 4). The fractal model was established as described in the Supplemental Experimental Procedures and was based on parameters measured in tissue sections in situ, including diameter and circumference of ductules, length of ductules (in micrometer and in number of cells), the ratio between lengths of consecutive branches, the ratio between radii of consecutive branches, and angles of branching. The model is dynamic and interactive; parameters can be changed to include different measurements.


Models of breast morphogenesis based on localization of stem cells in the developing mammary lobule.

Honeth G, Schiavinotto T, Vaggi F, Marlow R, Kanno T, Shinomiya I, Lombardi S, Buchupalli B, Graham R, Gazinska P, Ramalingam V, Burchell J, Purushotham AD, Pinder SE, Csikasz-Nagy A, Dontu G - Stem Cell Reports (2015)

Fractal Models of Mammary Lobules with Virtual Sections in Comparison to Real Tissue Sections(A) 3D trees generated by fractal modeling, representing mammary lobules with 6, 8, and 12 branching generations.(B) Virtual sections through the fractal trees shown in (A). The sectioning plane is indicated by the blue line in (A).(C) Real sections through normal breast lobules, corresponding to the fractal sections shown in (B) (H&E staining). Scale bar, 100 μm.See also Figure S5.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400614&req=5

fig4: Fractal Models of Mammary Lobules with Virtual Sections in Comparison to Real Tissue Sections(A) 3D trees generated by fractal modeling, representing mammary lobules with 6, 8, and 12 branching generations.(B) Virtual sections through the fractal trees shown in (A). The sectioning plane is indicated by the blue line in (A).(C) Real sections through normal breast lobules, corresponding to the fractal sections shown in (B) (H&E staining). Scale bar, 100 μm.See also Figure S5.
Mentions: To better elucidate the localization of stem cell markers in the 3D architecture of the mammary lobule, we utilized computer-generated 3D fractal models and compared virtual sections with equivalent immunostained tissue sections (Figure 4). The fractal model was established as described in the Supplemental Experimental Procedures and was based on parameters measured in tissue sections in situ, including diameter and circumference of ductules, length of ductules (in micrometer and in number of cells), the ratio between lengths of consecutive branches, the ratio between radii of consecutive branches, and angles of branching. The model is dynamic and interactive; parameters can be changed to include different measurements.

Bottom Line: However, the identity of these cells is a subject of controversy and their localization in the breast epithelium is not known.In this study, we utilized a novel approach to analyze the morphogenesis of mammary lobules, by combining one-dimensional theoretical models and computer-generated 3D fractals.An increased representation of stem cells was found in smaller, less developed lobules compared to larger, more mature lobules, with marked differences in the gland of iparous versus parous women and that of BRCA1/2 mutation carriers versus non-carriers.

View Article: PubMed Central - PubMed

Affiliation: Research Oncology, King's College London School of Medicine, London SE1 9RT, UK. Electronic address: gabriella.honeth@med.lu.se.

Show MeSH
Related in: MedlinePlus