Models of breast morphogenesis based on localization of stem cells in the developing mammary lobule.
Bottom Line: However, the identity of these cells is a subject of controversy and their localization in the breast epithelium is not known.In this study, we utilized a novel approach to analyze the morphogenesis of mammary lobules, by combining one-dimensional theoretical models and computer-generated 3D fractals.An increased representation of stem cells was found in smaller, less developed lobules compared to larger, more mature lobules, with marked differences in the gland of iparous versus parous women and that of BRCA1/2 mutation carriers versus non-carriers.
Affiliation: Research Oncology, King's College London School of Medicine, London SE1 9RT, UK. Electronic address: email@example.com.Show MeSH
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Mentions: To better elucidate the localization of stem cell markers in the 3D architecture of the mammary lobule, we utilized computer-generated 3D fractal models and compared virtual sections with equivalent immunostained tissue sections (Figure 4). The fractal model was established as described in the Supplemental Experimental Procedures and was based on parameters measured in tissue sections in situ, including diameter and circumference of ductules, length of ductules (in micrometer and in number of cells), the ratio between lengths of consecutive branches, the ratio between radii of consecutive branches, and angles of branching. The model is dynamic and interactive; parameters can be changed to include different measurements.
Affiliation: Research Oncology, King's College London School of Medicine, London SE1 9RT, UK. Electronic address: firstname.lastname@example.org.