Models of breast morphogenesis based on localization of stem cells in the developing mammary lobule.
Bottom Line: However, the identity of these cells is a subject of controversy and their localization in the breast epithelium is not known.In this study, we utilized a novel approach to analyze the morphogenesis of mammary lobules, by combining one-dimensional theoretical models and computer-generated 3D fractals.An increased representation of stem cells was found in smaller, less developed lobules compared to larger, more mature lobules, with marked differences in the gland of iparous versus parous women and that of BRCA1/2 mutation carriers versus non-carriers.
Affiliation: Research Oncology, King's College London School of Medicine, London SE1 9RT, UK. Electronic address: firstname.lastname@example.org.Show MeSH
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Mentions: The areas where ALDH1A1 was expressed were characterized by a less organized bilayer structure, often with no clear lumen, co-localization of basal (CK14, CK5/6) and luminal (CK19) cytokeratins in the same cells (Figures 2A and S4), and often lower expression of EpCAM and CD10 (Figures S4 and 2I) compared to the surrounding epithelium. Further characterization using markers for proliferation (Ki67, MCM2) and cell-cycle arrest (p27) indicated that these areas were mainly resting or quiescent (Figure 3).
Affiliation: Research Oncology, King's College London School of Medicine, London SE1 9RT, UK. Electronic address: email@example.com.