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Highly selective in vivo labeling of subcutaneous white adipocyte precursors with Prx1-Cre.

Sanchez-Gurmaches J, Hsiao WY, Guertin DA - Stem Cell Reports (2015)

Bottom Line: Here, we show that the majority of the precursor and mature subcutaneous white adipocytes in adult C57Bl/6 mice are labeled by Prx1-Cre.In sharp contrast, few to no brown adipocytes or visceral white adipocytes are marked by Prx1-Cre.This suggests that Prx1-Cre-mediated recombination may be useful for making depot-restricted genetic manipulations in subcutaneous white adipocyte precursor cells, particularly when targeting genes with fat-specific functions.

View Article: PubMed Central - PubMed

Affiliation: Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA.

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Most Brown Adipocytes Are Not Labeled by Prx1-Cre(A–E) Representative images of the indicated BATs of 6-week-old Prx1-Cre;R26R-mTmG male and female mice (top insert, tdTomato; bottom insert, mGFP). The number of tdTomato+ and mGFP+ mature brown adipocytes in each depot is indicated in a graph at the left side of each row (n = 3 per each sex; mean + SEM). For all panels, scale bar represents 50 μm.See also Figure S2.
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fig3: Most Brown Adipocytes Are Not Labeled by Prx1-Cre(A–E) Representative images of the indicated BATs of 6-week-old Prx1-Cre;R26R-mTmG male and female mice (top insert, tdTomato; bottom insert, mGFP). The number of tdTomato+ and mGFP+ mature brown adipocytes in each depot is indicated in a graph at the left side of each row (n = 3 per each sex; mean + SEM). For all panels, scale bar represents 50 μm.See also Figure S2.

Mentions: Given that almost no brown APCs in iBAT and sBAT express mGFP in Prx1-Cre;R26R-mTmG mice, we expected few if any mature brown adipocytes would be mGFP+. Indeed, no mGFP+ brown adipocytes are detectable in iBAT, sBAT, cBAT, or prBAT in either male or female Prx1-Cre;R26R-mTmG (Figures 3A–3D). However, in paBAT, mGFP is detectable in 64.3% and 62.2% of the cells in males and females, respectively (Figure 3E). Thus, in most major BAT depots, the brown adipocytes are not labeled with Prx1-Cre; however, in one depot (paBAT), Prx1-Cre marks the majority of the brown adipocytes.


Highly selective in vivo labeling of subcutaneous white adipocyte precursors with Prx1-Cre.

Sanchez-Gurmaches J, Hsiao WY, Guertin DA - Stem Cell Reports (2015)

Most Brown Adipocytes Are Not Labeled by Prx1-Cre(A–E) Representative images of the indicated BATs of 6-week-old Prx1-Cre;R26R-mTmG male and female mice (top insert, tdTomato; bottom insert, mGFP). The number of tdTomato+ and mGFP+ mature brown adipocytes in each depot is indicated in a graph at the left side of each row (n = 3 per each sex; mean + SEM). For all panels, scale bar represents 50 μm.See also Figure S2.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400610&req=5

fig3: Most Brown Adipocytes Are Not Labeled by Prx1-Cre(A–E) Representative images of the indicated BATs of 6-week-old Prx1-Cre;R26R-mTmG male and female mice (top insert, tdTomato; bottom insert, mGFP). The number of tdTomato+ and mGFP+ mature brown adipocytes in each depot is indicated in a graph at the left side of each row (n = 3 per each sex; mean + SEM). For all panels, scale bar represents 50 μm.See also Figure S2.
Mentions: Given that almost no brown APCs in iBAT and sBAT express mGFP in Prx1-Cre;R26R-mTmG mice, we expected few if any mature brown adipocytes would be mGFP+. Indeed, no mGFP+ brown adipocytes are detectable in iBAT, sBAT, cBAT, or prBAT in either male or female Prx1-Cre;R26R-mTmG (Figures 3A–3D). However, in paBAT, mGFP is detectable in 64.3% and 62.2% of the cells in males and females, respectively (Figure 3E). Thus, in most major BAT depots, the brown adipocytes are not labeled with Prx1-Cre; however, in one depot (paBAT), Prx1-Cre marks the majority of the brown adipocytes.

Bottom Line: Here, we show that the majority of the precursor and mature subcutaneous white adipocytes in adult C57Bl/6 mice are labeled by Prx1-Cre.In sharp contrast, few to no brown adipocytes or visceral white adipocytes are marked by Prx1-Cre.This suggests that Prx1-Cre-mediated recombination may be useful for making depot-restricted genetic manipulations in subcutaneous white adipocyte precursor cells, particularly when targeting genes with fat-specific functions.

View Article: PubMed Central - PubMed

Affiliation: Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA.

Show MeSH