Highly selective in vivo labeling of subcutaneous white adipocyte precursors with Prx1-Cre.
Bottom Line: Here, we show that the majority of the precursor and mature subcutaneous white adipocytes in adult C57Bl/6 mice are labeled by Prx1-Cre.In sharp contrast, few to no brown adipocytes or visceral white adipocytes are marked by Prx1-Cre.This suggests that Prx1-Cre-mediated recombination may be useful for making depot-restricted genetic manipulations in subcutaneous white adipocyte precursor cells, particularly when targeting genes with fat-specific functions.
Affiliation: Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA.Show MeSH
Mentions: Given that almost no brown APCs in iBAT and sBAT express mGFP in Prx1-Cre;R26R-mTmG mice, we expected few if any mature brown adipocytes would be mGFP+. Indeed, no mGFP+ brown adipocytes are detectable in iBAT, sBAT, cBAT, or prBAT in either male or female Prx1-Cre;R26R-mTmG (Figures 3A–3D). However, in paBAT, mGFP is detectable in 64.3% and 62.2% of the cells in males and females, respectively (Figure 3E). Thus, in most major BAT depots, the brown adipocytes are not labeled with Prx1-Cre; however, in one depot (paBAT), Prx1-Cre marks the majority of the brown adipocytes.
Affiliation: Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA.