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Highly selective in vivo labeling of subcutaneous white adipocyte precursors with Prx1-Cre.

Sanchez-Gurmaches J, Hsiao WY, Guertin DA - Stem Cell Reports (2015)

Bottom Line: Here, we show that the majority of the precursor and mature subcutaneous white adipocytes in adult C57Bl/6 mice are labeled by Prx1-Cre.In sharp contrast, few to no brown adipocytes or visceral white adipocytes are marked by Prx1-Cre.This suggests that Prx1-Cre-mediated recombination may be useful for making depot-restricted genetic manipulations in subcutaneous white adipocyte precursor cells, particularly when targeting genes with fat-specific functions.

View Article: PubMed Central - PubMed

Affiliation: Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA.

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Prx1-Cre Labels Mature Subcutaneous White Adipocytes(Aā€“F) Representative images of the indicated WATs of 6-week-old Prx1-Cre;R26R-mTmG male and female mice (top insert, tdTomato; bottom insert, mGFP). The number of tdTomato+ and mGFP+ mature white adipocytes in each depot is indicated in a graph at the left side of each row (nĀ = 3 per each sex; meanĀ + SEM). For all panels, scale bar represents 50Ā Ī¼m.See also FigureĀ S2.
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fig2: Prx1-Cre Labels Mature Subcutaneous White Adipocytes(Aā€“F) Representative images of the indicated WATs of 6-week-old Prx1-Cre;R26R-mTmG male and female mice (top insert, tdTomato; bottom insert, mGFP). The number of tdTomato+ and mGFP+ mature white adipocytes in each depot is indicated in a graph at the left side of each row (nĀ = 3 per each sex; meanĀ + SEM). For all panels, scale bar represents 50Ā Ī¼m.See also FigureĀ S2.

Mentions: To quantify the extent to which Prx1-Cre-labeled APCs contribute to the mature adipocyte population, we examined each depot by confocal microscopy. In the asWAT, the labeling pattern is heterogeneous (Figures 2A and 2B), consistent with the mixed mGFP+ and tdTomato+ APC labeling in this depot (FigureĀ 1B). Notably, the labeling pattern in asWAT varies with anatomical location; for example, the dorsal region of the depot is tdTomato+ (FigureĀ 2A), whereas the ventral region of the depot contains a variegated mixture of mGFP+ and tdTomato+ adipocytes (FigureĀ 2B). On the other hand, 96% of the adipocytes in psWAT are mGFP+ (FigureĀ 2C), which is consistent with the high percentage of mGFP+ APCs in this depot (FigureĀ 1C). However, because psWAT is roughly 1.5ā€“2.0 times the mass of asWAT, these results indicate that most of the subcutaneous white adipocytes are mGFP in Prx1-Cre;R26R-mTmG mice.


Highly selective in vivo labeling of subcutaneous white adipocyte precursors with Prx1-Cre.

Sanchez-Gurmaches J, Hsiao WY, Guertin DA - Stem Cell Reports (2015)

Prx1-Cre Labels Mature Subcutaneous White Adipocytes(Aā€“F) Representative images of the indicated WATs of 6-week-old Prx1-Cre;R26R-mTmG male and female mice (top insert, tdTomato; bottom insert, mGFP). The number of tdTomato+ and mGFP+ mature white adipocytes in each depot is indicated in a graph at the left side of each row (nĀ = 3 per each sex; meanĀ + SEM). For all panels, scale bar represents 50Ā Ī¼m.See also FigureĀ S2.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4400610&req=5

fig2: Prx1-Cre Labels Mature Subcutaneous White Adipocytes(Aā€“F) Representative images of the indicated WATs of 6-week-old Prx1-Cre;R26R-mTmG male and female mice (top insert, tdTomato; bottom insert, mGFP). The number of tdTomato+ and mGFP+ mature white adipocytes in each depot is indicated in a graph at the left side of each row (nĀ = 3 per each sex; meanĀ + SEM). For all panels, scale bar represents 50Ā Ī¼m.See also FigureĀ S2.
Mentions: To quantify the extent to which Prx1-Cre-labeled APCs contribute to the mature adipocyte population, we examined each depot by confocal microscopy. In the asWAT, the labeling pattern is heterogeneous (Figures 2A and 2B), consistent with the mixed mGFP+ and tdTomato+ APC labeling in this depot (FigureĀ 1B). Notably, the labeling pattern in asWAT varies with anatomical location; for example, the dorsal region of the depot is tdTomato+ (FigureĀ 2A), whereas the ventral region of the depot contains a variegated mixture of mGFP+ and tdTomato+ adipocytes (FigureĀ 2B). On the other hand, 96% of the adipocytes in psWAT are mGFP+ (FigureĀ 2C), which is consistent with the high percentage of mGFP+ APCs in this depot (FigureĀ 1C). However, because psWAT is roughly 1.5ā€“2.0 times the mass of asWAT, these results indicate that most of the subcutaneous white adipocytes are mGFP in Prx1-Cre;R26R-mTmG mice.

Bottom Line: Here, we show that the majority of the precursor and mature subcutaneous white adipocytes in adult C57Bl/6 mice are labeled by Prx1-Cre.In sharp contrast, few to no brown adipocytes or visceral white adipocytes are marked by Prx1-Cre.This suggests that Prx1-Cre-mediated recombination may be useful for making depot-restricted genetic manipulations in subcutaneous white adipocyte precursor cells, particularly when targeting genes with fat-specific functions.

View Article: PubMed Central - PubMed

Affiliation: Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA.

Show MeSH