Highly selective in vivo labeling of subcutaneous white

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Highly selective in vivo labeling of subcutaneous white adipocyte precursors with Prx1-Cre. Sanchez-Gurmaches J, Hsiao WY, Guertin DA - Stem Cell Reports (2015) Bottom Line: Here, we show that the majority of the precursor and mature subcutaneous white adipocytes in adult C57Bl/6 mice are labeled by Prx1-Cre.In sharp contrast, few to no brown adipocytes or visceral white adipocytes are marked by Prx1-Cre.This suggests that Prx1-Cre-mediated recombination may be useful for making depot-restricted genetic manipulations in subcutaneous white adipocyte precursor cells, particularly when targeting genes with fat-specific functions. View Article: PubMed Central - PubMed Affiliation: Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA. Show MeSH Major Adipocytes, White/cytology/metabolism* Homeodomain Proteins/genetics* Homologous Recombination* Integrases/metabolism* Subcutaneous Fat/cytology* Minor Adipocytes, Brown/metabolism Animals Female Gene Expression Gene Targeting/methods Male Mice Mice, Transgenic	© Copyright Policy - CC BY-NC-ND Related In: Results - Collection License Show All Figures getmorefigures.php?uid=PMC4400610&req=5

Highly selective in vivo labeling of subcutaneous white adipocyte precursors with Prx1-Cre.

Sanchez-Gurmaches J, Hsiao WY, Guertin DA - Stem Cell Reports (2015)

Bottom Line: Here, we show that the majority of the precursor and mature subcutaneous white adipocytes in adult C57Bl/6 mice are labeled by Prx1-Cre.In sharp contrast, few to no brown adipocytes or visceral white adipocytes are marked by Prx1-Cre.This suggests that Prx1-Cre-mediated recombination may be useful for making depot-restricted genetic manipulations in subcutaneous white adipocyte precursor cells, particularly when targeting genes with fat-specific functions.

View Article: PubMed Central - PubMed

Affiliation: Program in Molecular Medicine, University of Massachusetts Medical School, 373 Plantation Street, Worcester, MA 01605, USA.