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RHAMM deficiency disrupts folliculogenesis resulting in female hypofertility.

Li H, Moll J, Winkler A, Frappart L, Brunet S, Hamann J, Kroll T, Verlhac MH, Heuer H, Herrlich P, Ploubidou A - Biol Open (2015)

Bottom Line: This resulted in folliculogenesis defects and female hypofertility, although HA-induced signalling was not affected.Deletion of the RHAMM C-terminus in vivo abolishes its spindle association, resulting in impaired spindle orientation in the dividing granulosa cells, folliculogenesis defects and subsequent female hypofertility.These data reveal the first identified physiological function for RHAMM, during oogenesis, and the importance of this spindle-associated function for female fertility.

View Article: PubMed Central - PubMed

Affiliation: Leibniz Institute for Age Research - Fritz Lipmann Institute, Beutenbergstrasse 11, D-07745 Jena, Germany.

No MeSH data available.


Related in: MedlinePlus

RHAMM deficiency has no adverse effects on primordial follicle formation.Visualization (A–F) and quantification (G) of primordial follicles in mouse ovaries, at post-natal day 7. (A–F) Hematoxylin- and eosin-stained representative sections of ovaries from hmmr+/+ (A) and hmmrm/m (D) females. Boxes indicate the magnified areas. No morphological defects were observed in hmmrm/m follicles (D–F) as compared to controls (A–C). (G) Quantification of primordial follicles, in (one every five) 4-µm-thick serial sections of ovaries of hmmr+/+ (n = 3) and hmmrm/m (n = 3) mice, showed no statistically significant difference in the number of these follicles between the genotypes. Data presented as mean±s.d. Scale bars: 100 µm (A,B,D,E); 10 µm (C,F).
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f03: RHAMM deficiency has no adverse effects on primordial follicle formation.Visualization (A–F) and quantification (G) of primordial follicles in mouse ovaries, at post-natal day 7. (A–F) Hematoxylin- and eosin-stained representative sections of ovaries from hmmr+/+ (A) and hmmrm/m (D) females. Boxes indicate the magnified areas. No morphological defects were observed in hmmrm/m follicles (D–F) as compared to controls (A–C). (G) Quantification of primordial follicles, in (one every five) 4-µm-thick serial sections of ovaries of hmmr+/+ (n = 3) and hmmrm/m (n = 3) mice, showed no statistically significant difference in the number of these follicles between the genotypes. Data presented as mean±s.d. Scale bars: 100 µm (A,B,D,E); 10 µm (C,F).

Mentions: In order to determine whether the hmmrm/m ovaries have a diminished reservoir of oocytes, the ovaries of mice at post-natal day 7 (PND7) were analyzed (Fig. 3). At this time point, primordial follicles' formation has been completed and the total number of oocytes available for reproduction is fixed (Pepling and Spradling, 2001; Pepling, 2006). The primordial follicles in hmmrm/m ovaries appear morphologically normal (Fig. 3D–F). Their quantification (Fig. 3G) indicated a potentially reduced reservoir of oocytes in hmmrm/m mutants but no statistically significant difference in the number of these follicles between mutant and wild-type animals.


RHAMM deficiency disrupts folliculogenesis resulting in female hypofertility.

Li H, Moll J, Winkler A, Frappart L, Brunet S, Hamann J, Kroll T, Verlhac MH, Heuer H, Herrlich P, Ploubidou A - Biol Open (2015)

RHAMM deficiency has no adverse effects on primordial follicle formation.Visualization (A–F) and quantification (G) of primordial follicles in mouse ovaries, at post-natal day 7. (A–F) Hematoxylin- and eosin-stained representative sections of ovaries from hmmr+/+ (A) and hmmrm/m (D) females. Boxes indicate the magnified areas. No morphological defects were observed in hmmrm/m follicles (D–F) as compared to controls (A–C). (G) Quantification of primordial follicles, in (one every five) 4-µm-thick serial sections of ovaries of hmmr+/+ (n = 3) and hmmrm/m (n = 3) mice, showed no statistically significant difference in the number of these follicles between the genotypes. Data presented as mean±s.d. Scale bars: 100 µm (A,B,D,E); 10 µm (C,F).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4400598&req=5

f03: RHAMM deficiency has no adverse effects on primordial follicle formation.Visualization (A–F) and quantification (G) of primordial follicles in mouse ovaries, at post-natal day 7. (A–F) Hematoxylin- and eosin-stained representative sections of ovaries from hmmr+/+ (A) and hmmrm/m (D) females. Boxes indicate the magnified areas. No morphological defects were observed in hmmrm/m follicles (D–F) as compared to controls (A–C). (G) Quantification of primordial follicles, in (one every five) 4-µm-thick serial sections of ovaries of hmmr+/+ (n = 3) and hmmrm/m (n = 3) mice, showed no statistically significant difference in the number of these follicles between the genotypes. Data presented as mean±s.d. Scale bars: 100 µm (A,B,D,E); 10 µm (C,F).
Mentions: In order to determine whether the hmmrm/m ovaries have a diminished reservoir of oocytes, the ovaries of mice at post-natal day 7 (PND7) were analyzed (Fig. 3). At this time point, primordial follicles' formation has been completed and the total number of oocytes available for reproduction is fixed (Pepling and Spradling, 2001; Pepling, 2006). The primordial follicles in hmmrm/m ovaries appear morphologically normal (Fig. 3D–F). Their quantification (Fig. 3G) indicated a potentially reduced reservoir of oocytes in hmmrm/m mutants but no statistically significant difference in the number of these follicles between mutant and wild-type animals.

Bottom Line: This resulted in folliculogenesis defects and female hypofertility, although HA-induced signalling was not affected.Deletion of the RHAMM C-terminus in vivo abolishes its spindle association, resulting in impaired spindle orientation in the dividing granulosa cells, folliculogenesis defects and subsequent female hypofertility.These data reveal the first identified physiological function for RHAMM, during oogenesis, and the importance of this spindle-associated function for female fertility.

View Article: PubMed Central - PubMed

Affiliation: Leibniz Institute for Age Research - Fritz Lipmann Institute, Beutenbergstrasse 11, D-07745 Jena, Germany.

No MeSH data available.


Related in: MedlinePlus